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Application of methyl ferulic acid in preparation of medicine for preventing and treating alcoholic liver disease

A technology of methyl ferulic acid and alcoholic liver disease, applied in the field of medicine, can solve the problems of prevention and treatment that have not been reported

Inactive Publication Date: 2016-05-18
GUILIN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The preventive and therapeutic effects of methyl ferulic acid on ethanol-induced alcoholic liver disease have not been reported

Method used

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  • Application of methyl ferulic acid in preparation of medicine for preventing and treating alcoholic liver disease
  • Application of methyl ferulic acid in preparation of medicine for preventing and treating alcoholic liver disease
  • Application of methyl ferulic acid in preparation of medicine for preventing and treating alcoholic liver disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1 Effect of methyl ferulic acid on ethanol-induced human normal liver cell line (L-02)

[0014] 1.1 Observation of different concentrations of methyl ferulic acid on the survival rate of L-02 cells.

[0015] Inoculate logarithmic phase cells in 96-well plate, 5000 cells per well, add a certain concentration of methyl ferulic acid after 24 hours, set 5 duplicate holes for each drug concentration, add MTT (5mg / ml) after 44 hours of drug addition Continue to incubate with 20ul for 4h, pour out the medium, blot dry, add 200ul of dimethyl sulfoxide to each well, mix well and then set an automatic microplate reader (detection wavelength 490nm) to read the optical density value (OD value) of each well and record the results , and calculate the survival rate. Survival rate (%)=(experimental group-blank control group) / (normal control group-blank control)×100%. The results are shown in Table 1.

[0016] Table 1. The effect of methyl ferulic acid on the survival rate of...

Embodiment 2

[0031] Example 2 The protective effect of methyl ferulic acid on the liver of acute alcoholic liver disease rat model:

[0032] 1. Drugs and animals

[0033] 1.1 Animals 60 SD rats, half male and half male, weighing 200±20g

[0034] 1.2 Grouping of animals: Randomly divide the animals into 6 groups, 10 in each group.

[0035] 1.3 Test drugs

[0036] (1) Bifendate, which is a traditional liver-protecting drug, is a drug approved by SFDA for marketing, and is used as a positive control drug;

[0037] (2) Methyl ferulic acid is a test drug to observe its protective effect on the liver of rat acute alcoholic liver disease model;

[0038] (3) Drug vehicle: 1% sodium carboxymethylcellulose solution.

Embodiment approach

[0040] The 1st group is the normal control group: intragastric administration of drug vehicle, the dose is 10ml / kg;

[0041] The 2nd group is the model control group: intragastric administration of drug solvent, the dose is 10ml / kg;

[0042]The third group is the bifendate treatment group: intragastric administration of bifendate at a dose of 200 mg / kg;

[0043] The 4th, 5th, and 6th groups were respectively low, medium, and high doses of methyl ferulic acid treatment groups: intragastric administration of methyl ferulic acid, and the doses were 10 mg / kg, 50 mg / kg, and 100 mg / kg respectively.

[0044] From the 4th day onwards, except the blank group, the rest of the groups were administered normally in the morning, 10ml / kg of 60% alcohol was administered in the afternoon, and the food and water were not limited. The alcohol was administered continuously for 10 days, and 1 hour after the end of the last oral administration of 60% alcohol. , anesthetized, and then blood and liv...

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PUM

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Abstract

The invention discloses an application of methyl ferulic acid in preparation of a medicine for preventing and treating alcoholic liver disease. An ethanol induced L-02 cell damage model proves that the methyl ferulic acid can reduce the MDA level and improve the activity of SOD, GSH-Px and CAT. According to animal experiments, the methyl ferulic acid can remarkably reduce the contents of ALT, AST and MDA of the rat suffering acute alcoholic liver injury and obviously improve the activity of SOD, GSH-Px and CAT; and the methyl ferulic acid can obviously reduce the content of triglyceride (TG), ALT, AST and MDA of the rat suffering chronic alcoholic liver injury and noticeably improve the activity of SOD, GSH-Px and CAT. The results of the cell and animal experiments indicate that the methyl ferulic acid has an exact effect in preventing and treating alcoholic liver disease and can be used for preparing a medicine for preventing and treating alcoholic liver disease independently or in combination.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of methyl ferulic acid in the preparation of drugs for the prevention and treatment of alcoholic liver disease. Background technique [0002] Alcoholic liver disease (alcoholic liver disease ALD) is a liver disease caused by long-term heavy drinking. It usually manifests as fatty liver at the initial stage. If the damage persists, it can develop into alcoholic hepatitis, liver fibrosis and cirrhosis. Severe alcoholism can induce widespread disease. Liver cell necrosis and even liver failure. Alcohol abuse and alcohol dependence have become increasingly serious public health problems in the world today. Alcoholic liver disease is the second most serious liver disease after viral hepatitis in my country. With the improvement of the lifestyle and dietary structure of Chinese residents and the increase of alcohol production and varieties, the proportion of alcoholic...

Claims

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Application Information

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IPC IPC(8): A61K31/192A61P1/16A61K36/69
CPCA61K31/192A61K36/69
Inventor 李勇文李丽杨成芳吴丹钟毓娟陈莉
Owner GUILIN MEDICAL UNIVERSITY
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