Composition for promoting synthesis of hyaluronic acid comprising Taraxacum herbs extracts and the use thereof

A technology of dandelion extract and hyaluronic acid, applied in pharmaceutical excipient compositions for preventing or improving arthritis, protecting joints, improving skin elasticity, food composition, food composition or pharmaceutical excipient composition, preventing or Cosmetic material composition for improving skin wrinkles, cosmetic material composition for skin moisturizing, can solve problems such as undiscovered research results, achieve the effect of improving skin elasticity, preventing or treating arthritis

Active Publication Date: 2016-05-18
LG HOUSEHOLD & HEALTH CARE LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, research on increasing the production of hyaluronic acid in the human body is in full swing, however, no remarkable research results have been found

Method used

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  • Composition for promoting synthesis of hyaluronic acid comprising Taraxacum herbs extracts and the use thereof
  • Composition for promoting synthesis of hyaluronic acid comprising Taraxacum herbs extracts and the use thereof
  • Composition for promoting synthesis of hyaluronic acid comprising Taraxacum herbs extracts and the use thereof

Examples

Experimental program
Comparison scheme
Effect test

manufacture example 1

[0116] Production example 1. Production of dandelion extract

[0117] The dandelion extract used in the following examples is an ethanol extract purchased from OBMRAP CO., LTD.

Embodiment 1

[0118] Example 1. Cytotoxicity Evaluation of Dandelion Extract

[0119] In order to evaluate the cytotoxicity of the dandelion extract, the cell division of human fibroblast-like synoviocytes (Human Fibroblast-like Synoviocyte, HFLS-OA, Cell Applications) collected from degenerative arthritis patients was measured using CellCountingKit-8 (DojindoLab, USA). Variety.

[0120] First, a uniform number of cells were plated on a 96-well plate (96wellplate) and stabilized, treated with dandelion extract to reach 5ppm and 10ppm, and cultured for another 24 hours. Then, CCK-8 treatment was performed, and the absorbance at 450 nm was measured 2 hours later. The relative cell viability of the dandelion extract-treated cells to the non-treated group (control group) was analyzed as described below, and the results are shown in Table 1 below.

[0121] 【Table 1】

[0122]

[0123] *repeats=3

[0124] *Relative cell viability=(extract treatment group) / (no treatment group)×100

[0125] ...

Embodiment 2

[0126] Example 2. Increased concentration of hyaluronic acid based on dandelion extract in synoviocytes of patients with degenerative arthritis

[0127] Human fibroblast-like synoviocytes from patients with degenerative arthritis were cultured by synoviocyte growth medium (Synoviocyte Growth Medium, Cell Applications), and 2.0×10 3 cells. After culturing for 3 days, 200 μL of synoviocyte growth medium was used for exchange, and different concentrations (5 ppm and 10 ppm) of dandelion extract were added. As a negative control group, it was treated with an equal amount of ethanol, and as a positive control group, it was treated with 1000 ppm of glucosamine hydrochloride (Sigma-aldrich), which is known to promote the production of hyaluronic acid. After culturing for 24 hours, the cell culture solution was recovered, and the concentration of hyaluronic acid was measured with a Hyaluronan ELISA kit (R&D Systems). The results are shown in Table 2.

[0128] 【Table 2】

[0129] ...

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PUM

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Abstract

Taraxacum herbs extracts or a fraction thereof effectively improve hyaluronic acid and expression of hyaluronic acid synthetase. Therefore a composition for promoting synthesis of the hyaluronic acid comprising Taraxacum herbs extracts can be effectively applied for protecting joints, preventing or treating arthritis, improving skin elasticity, preventing aging, preventing or treating skin wrinkle and skin moisturizing.

Description

technical field [0001] The present invention relates to a hyaluronic acid production-promoting composition containing a dandelion (Taraxacum spp.) extract or a fraction thereof as an active ingredient. [0002] In addition, the present invention relates to a pharmaceutical composition for protecting joints, preventing or treating arthritis, a pharmaceutical excipient composition for protecting joints, preventing or improving arthritis, or a food composition containing the above composition as an active ingredient . [0003] In addition, the present invention also relates to a cosmetic composition for improving skin elasticity, preventing aging, preventing or improving skin wrinkles, or a food composition containing a composition for promoting hyaluronic acid production as an active ingredient; A pharmaceutical composition for preventing or treating skin wrinkles. [0004] In addition, the present invention relates to a skin moisturizing cosmetic composition, a food compositi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/288A61P19/02A61P29/00A61P17/00A61K8/97A61Q19/00A61Q19/08A23L33/105
CPCA61P17/00A61P19/02A61Q19/007A61Q19/08A23L33/105A61K8/9789A61K36/288
Inventor 李宝永金允善赵浩成李相和
Owner LG HOUSEHOLD & HEALTH CARE LTD
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