Method for extracting phospholipid from heads of hypophthalmichthys molitrix and product of method
A silver carp fish head and fish head phospholipid technology, applied in the method and its products, the field of extracting phospholipids from silver carp fish head, can solve the problems of high cost, difficulty in realizing recycling, affecting the extraction effect of organic solvents, etc., and achieve high-efficiency extraction , important scientific value and economic benefits
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Embodiment 1
[0029] Fresh silver carp heads were chopped into pieces of 1.5 cm square, weighed 200 g, and added 2000 mL of 95% ethanol at a mass-volume ratio of 1:10 (m:V, g / mL). The fish head fragments in the ethanol solution were crushed at high speed for 3 minutes by a flash extraction device to form a slurry, and the slurry was vacuum filtered to obtain an ethanol extract. The ethanol extract was vacuum rotary evaporated to remove ethanol, weighed the residue, added 1:5 (m:V, g / ml) acetone according to the mass volume ratio, stirred and mixed, then centrifuged in a horizontal centrifuge, and removed the acetone supernatant The acetone insoluble precipitate is obtained, which is a light yellow translucent viscous substance. After the residual acetone is removed by vacuum rotary evaporation, the precipitate is a silver carp head phospholipid product, and its phospholipid purity is 93%.
Embodiment 2
[0031] Frozen silver carp head was chopped into 2cm square fragments, weighed 10kg, and added 150L of 95% ethanol according to the mass volume ratio of 1:15 (m:V, g / mL). The fish head fragments in the ethanol liquid were crushed at high speed for 5 minutes by a flash extraction device to become a slurry, and the slurry was filtered by a plate and frame filter press to obtain an ethanol extract. Use a thin film evaporator to evaporate the ethanol extract to remove the ethanol, weigh the residue, add 1:10 (m:V, g / ml) acetone according to the mass volume ratio, stir and mix, use a disc centrifuge to separate, remove the acetone The acetone insoluble precipitate obtained from the supernatant is a pale yellow translucent viscous substance. After the residual acetone is removed by vacuum rotary evaporation, the precipitate is a silver carp fish head phospholipid product, and its phospholipid purity is 90%.
Embodiment 3
[0033] Frozen silver carp heads were chopped into pieces of 1.8 cm square, weighed 10 kg, and added 200 L of 95% ethanol at a mass-volume ratio of 1:20 (m:V, g / mL). The fish head fragments in the ethanol liquid were crushed at high speed for 5 minutes by a flash extraction device to become a slurry, and the slurry was filtered by a plate and frame filter press to obtain an ethanol extract. Use a thin film evaporator to evaporate the ethanol extract to remove the ethanol, weigh the residue, add 1:8 (m:V, g / ml) acetone according to the mass volume ratio, stir and mix, use a disc centrifuge to separate, remove the acetone The acetone-insoluble precipitate was obtained from the supernatant, which was a light yellow translucent viscous substance. After the residual acetone was removed by vacuum rotary evaporation, the silver carp head phospholipid product was obtained, and the phospholipid purity was 92%.
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