Method for extracting phospholipid from heads of hypophthalmichthys molitrix and product of method

A silver carp fish head and fish head phospholipid technology, applied in the method and its products, the field of extracting phospholipids from silver carp fish head, can solve the problems of high cost, difficulty in realizing recycling, affecting the extraction effect of organic solvents, etc., and achieve high-efficiency extraction , important scientific value and economic benefits

Active Publication Date: 2016-05-25
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aquatic products generally contain high water content (generally above 80%). The existence of water will affect the extraction effect of organic solvents. The raw materials need to be dried during extraction. Ordinary drying will cause lipid oxidation in raw materials, affecting The quality of the extracted phospholipids, freeze-drying can maintain the quality of the raw

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Fresh silver carp heads were chopped into pieces of 1.5 cm square, weighed 200 g, and added 2000 mL of 95% ethanol at a mass-volume ratio of 1:10 (m:V, g / mL). The fish head fragments in the ethanol solution were crushed at high speed for 3 minutes by a flash extraction device to form a slurry, and the slurry was vacuum filtered to obtain an ethanol extract. The ethanol extract was vacuum rotary evaporated to remove ethanol, weighed the residue, added 1:5 (m:V, g / ml) acetone according to the mass volume ratio, stirred and mixed, then centrifuged in a horizontal centrifuge, and removed the acetone supernatant The acetone insoluble precipitate is obtained, which is a light yellow translucent viscous substance. After the residual acetone is removed by vacuum rotary evaporation, the precipitate is a silver carp head phospholipid product, and its phospholipid purity is 93%.

Embodiment 2

[0031] Frozen silver carp head was chopped into 2cm square fragments, weighed 10kg, and added 150L of 95% ethanol according to the mass volume ratio of 1:15 (m:V, g / mL). The fish head fragments in the ethanol liquid were crushed at high speed for 5 minutes by a flash extraction device to become a slurry, and the slurry was filtered by a plate and frame filter press to obtain an ethanol extract. Use a thin film evaporator to evaporate the ethanol extract to remove the ethanol, weigh the residue, add 1:10 (m:V, g / ml) acetone according to the mass volume ratio, stir and mix, use a disc centrifuge to separate, remove the acetone The acetone insoluble precipitate obtained from the supernatant is a pale yellow translucent viscous substance. After the residual acetone is removed by vacuum rotary evaporation, the precipitate is a silver carp fish head phospholipid product, and its phospholipid purity is 90%.

Embodiment 3

[0033] Frozen silver carp heads were chopped into pieces of 1.8 cm square, weighed 10 kg, and added 200 L of 95% ethanol at a mass-volume ratio of 1:20 (m:V, g / mL). The fish head fragments in the ethanol liquid were crushed at high speed for 5 minutes by a flash extraction device to become a slurry, and the slurry was filtered by a plate and frame filter press to obtain an ethanol extract. Use a thin film evaporator to evaporate the ethanol extract to remove the ethanol, weigh the residue, add 1:8 (m:V, g / ml) acetone according to the mass volume ratio, stir and mix, use a disc centrifuge to separate, remove the acetone The acetone-insoluble precipitate was obtained from the supernatant, which was a light yellow translucent viscous substance. After the residual acetone was removed by vacuum rotary evaporation, the silver carp head phospholipid product was obtained, and the phospholipid purity was 92%.

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PUM

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Abstract

The invention belongs to the technical field of phospholipid extraction with a large amount of cultured freshwater fishes and particularly relates to a method for extracting phospholipid from heads of hypophthalmichthys molitrix and a product of the method. The method includes steps: 1) chopping cleaned heads of hypophthalmichthys molitrix into square fragments with the side length being 1-2cm; 2) mixing the fragments with ethanol with the volume fraction being 95% according to a mass volume ratio of 1:10-1:20 to obtain to-be-treated products; 3) subjecting the to-be-treated products to high-speed crushing of the fragments in a flash extraction device to obtain serous fluid; 4) filtering the serous fluid to obtain ethanol extract; 5) removing ethanol from the ethanol extract, mixing with acetone according to a mass volume ratio of 1:5-1:10, centrifuging, removing acetone supernatant, and removing residual acetone to obtain a finished product. High-efficiency extraction of the phospholipid from the heads of the hypophthalmichthys molitrix is realized by an extremely simple technical process, efficient process control and adoption of easily-acquirable extracting solution harmless to extract components.

Description

technical field [0001] The invention belongs to the technical field of extracting phospholipids from bulk cultured freshwater fish, and in particular relates to a method for extracting phospholipids from silver carp heads and a product thereof. Background technique [0002] China is one of the most developed countries of freshwater fishery in the world, with an annual output of more than 15 million tons of freshwater fish; Silvercarp (Hypophthalmichthysmolitrix), as one of the main freshwater fish species in my country, has strong adaptability, fast growth, and an annual output of 300 tons. However, due to the restrictions on the consumption habits of freshwater fish in the international and domestic markets, as well as the processing characteristics of the fish itself, the flavor and taste and other factors, its market value is relatively low, and it needs to be further developed and utilized. [0003] Domestic studies have found that the fatty acid composition of silver car...

Claims

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Application Information

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IPC IPC(8): C07F9/10
CPCC07F9/10C07F9/103
Inventor 王琦王文倩王学东张维农齐玉堂陈季旺王海滨
Owner WUHAN POLYTECHNIC UNIVERSITY
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