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Photoinduced dielectrophoresis assembly chip and method for constructing gradient-structure gel complex

A technology of dielectrophoresis and combination, which is applied in the field of biomedicine, can solve the problem that the construction of exogenous factor concentration gradient and cell concentration gradient is difficult to realize at the same time, so as to reduce complexity and cost, reduce lateral resistance, and maintain active effect

Inactive Publication Date: 2016-05-25
HOHAI UNIV CHANGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for assembling a chip by photo-induced dielectrophoresis and constructing a structurally gradient gel assembly that wraps cells, so as to solve the problem of exogenous There are technical problems that are difficult to realize simultaneously in the construction of the concentration gradient of sexually acting factors and the concentration gradient of cells

Method used

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  • Photoinduced dielectrophoresis assembly chip and method for constructing gradient-structure gel complex
  • Photoinduced dielectrophoresis assembly chip and method for constructing gradient-structure gel complex
  • Photoinduced dielectrophoresis assembly chip and method for constructing gradient-structure gel complex

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Embodiment 1

[0038] Such as figure 1 As shown, this embodiment 1 provides a photo-dielectrophoresis assembly chip, including: a number of injection ports (first injection port 1101, second injection port 1102, third injection port 1103, fourth injection port 1104), and several gel micro-blocks (first gel micro-block 191, second gel micro-block 192, and third gel micro-block 193) are adapted to be respectively injected into the microfluidic cavity 140 through corresponding injection ports; The upper end of the microfluidic cavity 140 is provided with an electrode layer 130, and the lower end is provided with a dummy photoelectrode forming layer 170.

[0039] Specifically, the electrode layer 130 includes a double-layer structure, from top to bottom: an upper transparent conductive layer 131, a hydrophobic coating 132; the virtual photoelectrode forming layer 170 is a multilayer structure, from top to bottom In order: the hydrophilic coating 171, the passivation layer 172, the photoconductive l...

Embodiment 2

[0044] Before assembling the structurally graded gel assembly, it is also necessary to prepare corresponding gel micro-blocks.

[0045] Such as figure 2 with image 3 As shown, the mold for preparing gel micro-blocks used in this embodiment includes a solid area 1, a hollow area 2, an upper cover sheet 3, and a lower cover sheet 4. First, the liquid gel precursor solution is injected into the hollow area 2 of the mold, and then through the gelation process, solid gel micro-blocks are formed in the hollow area of ​​the mold, and then the entire mold device (including upper and lower Coverslips) placed in phosphate buffer solution, after a few minutes of soaking, remove the upper and lower coverslips. Then the middle layer of the mold with the solid gel micro-blocks was taken out separately and put into a petri dish filled with a new phosphate buffer solution. After several minutes of shaking, the solid gel micro-blocks in the middle layer of the mold gradually separated from the ...

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Abstract

The invention relates to a photoinduced dielectrophoresis assembly chip and a method for constructing a gradient-structure gel complex. The method comprises the following steps: (S1) distributing multiple gel micro blocks with different structure attributes and wrapping cells in a photoinduced dielectrophoresis force field to form a gel micro-block complex; and (S2) guiding the large-scale self-assembly behavior of cells to realize a gradient structure of the gel micro-block complex. In the invention, only one way of sine excitation signals is needed while the required voltage is very low, thus the maintenance of cell activity is facilitated, and the cost is reduced. The method provides an extremely essential means for constructing a micro multi-cell structure and developing the control technology in the bio-manufacturing technology, and has a broad application prospect in the fields such as construction of an in-vitro tissue model and tissue engineering.

Description

Technical field [0001] The invention is a method for constructing a structure-graded gel body through microscopic force, and relates to the field of biomedicine, especially the field of constructing a structure that can imitate biological tissue structure in vitro through microscopic electric force. Background technique [0002] There are a large number of structural gradient features in natural biological tissues, that is, the compactness of the microstructure of the tissue gradually increases or decreases along a certain direction. These graded tissue structures play an important role in the realization of various physiological functions. Therefore, the construction of gradient microstructure or multicellular structure in vitro has become a hot spot in the research field of tissue regeneration. [0003] At present, an important method of constructing a gradient microstructure in vitro is to use hydrogel materials (natural or synthetic) to produce in vitro microscopic three-dimen...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12N5/00
CPCC12M21/08C12M33/00C12N5/0012C12N2533/76
Inventor 朱晓璐
Owner HOHAI UNIV CHANGZHOU
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