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Detection primer set of cyp3a5 gene, reaction system and application thereof

A technology of reaction system and detection primers, which is applied in the determination/testing of microorganisms, recombinant DNA technology, biochemical equipment and methods, etc., can solve the problems of unfavorable detection efficiency, clinical popularization, high cost of purchasing equipment, and huge detection demand. Achieve the effects of high yield, easy identification and broad application prospects

Active Publication Date: 2019-01-11
BEIJING JINQI BIOLOGICAL TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of these methods for CYP3A5 genotyping detection has problems such as high equipment cost, complicated operation procedures, and long detection cycle to varying degrees, which is not conducive to improving detection efficiency and achieving clinical popularization.
However, from the current point of view, the demand for this kind of detection is very huge. Therefore, finding an efficient and affordable detection method that is easily accepted by patients has important practical significance for CYP3A5 genotyping detection

Method used

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  • Detection primer set of cyp3a5 gene, reaction system and application thereof
  • Detection primer set of cyp3a5 gene, reaction system and application thereof
  • Detection primer set of cyp3a5 gene, reaction system and application thereof

Examples

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Effect test

Embodiment 1

[0060] Extract 1ml of venous blood from 4 people and store it in EDTA anticoagulant tubes. Take 200ul of blood from each tube, use DNA extraction kits from Quanshijin or other companies, refer to the kit instructions to extract whole genome DNA as a template for detection, and mark them as M1, M2, M3 and M4. React with the corresponding primer set in the present invention to verify the implementation effect of the present invention.

[0061] Using Allele-Specific LAMP technology for template M, the nucleic acid to be detected is subjected to LAMP reaction, which is divided into two groups of reaction systems, one group is system A for A allele, and the other is system G for G allele. Template Mn (n is group number 1-4) was added to system A and system G respectively to obtain reaction systems A and G.

[0062] First, select the primer set as described in the first aspect of the application to react the sequence of the sample, and the primer set is as follows:

[0063] Forwar...

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Abstract

The invention belongs to the technical field of biological detection, and in particular, relates to a CYP3A5 gene detection primer group and a reaction system composed thereof; the detection primer group comprises a forward outer primer F3A shown as SEQ ID NO:1, a reverse outer primer B3A shown as SEQ ID NO:2, a forward inner primer FIPA shown as SEQ ID NO:3, a reverse inner primer BIPA shown as SEQ ID NO:4, a forward outer primer F3G shown as SEQ ID NO:5, a reverse outer primer B3G shown as SEQ ID NO:6, a forward inner primer FIPG shown as SEQ ID NO:7, and a reverse inner primer BIPG shown as SEQ ID NO:8. The reaction system has the advantages of simple steps, rapid amplification reaction, high efficiency, high specificity and simple identification, so the reaction system has a wider application prospect.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a CYP3A5 gene detection primer set, a reaction system formed by it and an application thereof. Background technique [0002] Cytochrome P450 (cytochrome P450 or CYP450) referred to as CYP450, mainly exists in the liver and intestinal monooxygenase, is a group of superfamily enzymes involved in the metabolism of endogenous substances and exogenous compounds in the human body, especially in the drug play an important role in the metabolic process in the body. Among them, CYP3A5 is involved in the metabolism of various drugs such as tacrolimus, midazolam, dapsone, cortisone, and nifedipine. A SNP of this gene, rs776746 (CYP3A5*3, 6986A>G), will cause the mRNA of CYP3A5 gene to be cut too short to form an "incomplete" CYP3A5 protein, which will lead to the reduction or even disappearance of CYP3A5 enzyme protein activity. [0003] According to statistics...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/6883
Inventor 张井存汪大为
Owner BEIJING JINQI BIOLOGICAL TECH CO LTD
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