A preparation method of sialylated Enteromorpha oligosaccharides that promotes the proliferation of intestinal probiotics
A technology of enteromorpha oligosaccharides and sialylation, which is applied to chemicals used for biological control, medical preparations containing active ingredients, pharmaceutical formulas, etc., to achieve the effect of increasing commodity value and economic added value, and improving efficiency
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Embodiment 1
[0021] (1) After drying, enteromorpha was ultrafinely crushed and passed through an 80-mesh sieve to obtain a dry powder. Add 60% ethanol to the dry powder of Enteromorpha and reflux for extraction for 1 hour. The ratio of dry powder weight to ethanol volume was 1:20. Recycle and remove ethanol after the reflux Recycle, dry, and pulverize to obtain dry defatted Enteromorpha powder;
[0022] (2) Add distilled water to the degreased Enteromorpha powder prepared in step (1), wherein the ratio of the mass of Enteromorpha to the volume of distilled water is 1:20, extract with 450W microwave at an extraction temperature of 60°C for 2 min, and filter after the extraction , the filter residue was extracted twice by repeating the above steps, the obtained filtrate was mixed and concentrated, filtered with suction, the filtrate was concentrated under reduced pressure to 3% of the original volume, and then pulverized after vacuum freeze-drying;
[0023] (3) Add distilled water to the pow...
Embodiment 2
[0032] (1) After drying, enteromorpha was ultrafinely pulverized and sieved through a 100-mesh sieve to obtain a dry powder. Add 80% ethanol to the dry powder of Enteromorpha and reflux for extraction for 2 hours. Ethanol is recycled, dried, and pulverized to obtain dry and defatted Enteromorpha powder;
[0033] (2) Add distilled water to the degreased Enteromorpha powder prepared in step (1), wherein the mass ratio of Enteromorpha mass to distilled water volume is 1:25, and 600 W microwave-assisted extraction is performed at an extraction temperature of 70°C for 3 min. After the extraction is completed, carry out Filtrate, repeat the above steps to extract the filter residue for 3 times, mix the obtained filtrate and concentrate, filter with suction, concentrate the filtrate under reduced pressure to 8% of the original volume, vacuum freeze-dry and then pulverize;
[0034] (3) Add distilled water to the powder obtained in step (2), wherein the volume ratio of powder mass to d...
Embodiment 3
[0043] (1) After drying, enteromorpha was ultrafinely pulverized and sieved through a 90-mesh sieve to obtain a dry powder. Add 70% ethanol to the dry powder of Enteromorpha reflux and extract for 1.5 hours. The ratio of dry powder weight to ethanol volume was 1:25, and recovered and removed after reflux Ethanol is recycled, dried, and pulverized to obtain dry and defatted Enteromorpha powder;
[0044] (2) Add distilled water to the degreased Enteromorpha powder prepared in step (1), wherein the ratio of the mass of Enteromorpha to the volume of distilled water is 1:23, 500 W microwave-assisted extraction at 65 °C for 3 min, after the extraction is completed, carry out Filtrate, repeat the above steps to extract the filter residue 3 times, mix the obtained filtrate, concentrate, filter with suction, concentrate the filtrate to 5% of the original volume under reduced pressure, vacuum freeze-dry and then pulverize;
[0045] (3) Add distilled water to the powder obtained in step ...
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