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Ultraviolet spectrophotometry for polysaccharide content in medicine material fokien angiopteris rhizomes

A technology for polysaccharides and polysaccharide content of horseshoe fern, which is applied in the direction of color/spectral characteristic measurement, etc.

Active Publication Date: 2016-05-25
GUANGXI INST OF CHINESE MEDICINE & PHARMA SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] As a commonly used national medicinal material, calla fern has been successively included in "Chinese Modern Yao Medicine" and "Quality Standards for Zhuang Medicine" (Volume 3), but there are only simple physical and chemical identifications in the standards

Method used

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  • Ultraviolet spectrophotometry for polysaccharide content in medicine material fokien angiopteris rhizomes
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  • Ultraviolet spectrophotometry for polysaccharide content in medicine material fokien angiopteris rhizomes

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preparation example Construction

[0023] 2.1 Preparation of calla fern polysaccharide extract

[0024] A. Grind 1 kg of calla fern into coarse powder, add 8 L of 60% ethanol, heat and reflux twice for 2 hours each time, filter, discard the filtrate, collect medicinal residues, and remove fat-soluble components.

[0025] Add 0.3 mol / L dilute hydrochloric acid to 8 L of B medicinal residues, heat and reflux to extract twice, each time for 2 hours, filter, discard medicinal residues, collect the filtrate and concentrate to obtain 150 g of ointment.

[0026] Add ethanol to the C concentrated solution to make the ethanol concentration reach 80%, let stand overnight, filter, collect the precipitate, add water to dissolve the precipitate, filter, concentrate the filtrate into a paste, and vacuum freeze-dry to obtain the calla fern polysaccharide extract.

[0027] 2.2 Determination of polysaccharide content in calla fern polysaccharide extract

[0028] The calla fern polysaccharide extract is analyzed as the sample t...

Embodiment 1

[0042] Refer to the above assay method, but use 0.1% anthrone-sulfuric acid reagent to react and measure to obtain the polysaccharide content in the calla fern polysaccharide extract, and the results are shown in Table 3.

[0043] table 3

[0044] Sampling volume (ml)

Embodiment 2

[0046] Referring to the above assay method, but using 0.3% anthrone-sulfuric acid reagent for reaction and determination, the polysaccharide content in the calla fern polysaccharide extract was obtained, and the results are shown in Table 4.

[0047] Table 4

[0048] Sampling volume (ml)

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Abstract

The invention discloses ultraviolet spectrophotometry for the polysaccharide content in medicine material fokien angiopteris rhizomes. Glucosum anhydricum is used for control, and an ultraviolet spectrophotometer is used for measuring the polysaccharide content in fokien angiopteris rhizome polysaccharide extract. By means of the ultraviolet spectrophotometry, the quality detection standard of medicine material fokien angiopteris rhizomes is improved, and a foundation is laid for establishing the quality standard of medicine material fokien angiopteris rhizomes; in addition, the spectrophotometry is high in sensitivity, easy and convenient to implement and accurate in result; by application and popularization of the ultraviolet spectrophotometry, the quality of the medicine material fokien angiopteris rhizomes can be ensured, and it can be guaranteed that the medicine material fokien angiopteris rhizomes are safe, effective and stable in clinical medication.

Description

technical field [0001] The invention relates to the technical field of quality control of calla fern medicinal material, in particular to an ultraviolet spectrophotometric method for determining polysaccharide content in calla fern medicinal material. Background technique [0002] Horseshoe fern, Avalokitesvara plant Angiopteris fokiensis Hieron. As a national characteristic medicinal material in Guangxi, it belongs to both Zhuang medicine and Yao medicine. The Zhuang medicine is called Maenzdaezma, and the Yao medicine is called Ma Tiya. It is usually used as a rhizome medicine. It is mainly distributed in Jinxiu, Gongcheng, Mashan and other places in Guangxi. Intestinal ulcer, pulmonary tuberculosis hemoptysis, nephritis edema, functional uterine bleeding, rheumatoid arthritis, lymph node tuberculosis, lymphadenitis, bruises, carbuncle sores, gunshot wounds, poisonous snakes and insect bites. For a long time, horseshoe fern has been one of the commonly used medicinal mate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33
CPCG01N21/33
Inventor 张赟赟杨海船李燕婧李嘉姜平川
Owner GUANGXI INST OF CHINESE MEDICINE & PHARMA SCI
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