Microfluidic chip-based tumor brain metastasis model building method and use
A technology of microfluidic chip and brain metastasis, which is applied in the field of cell biology and tissue engineering, and can solve the problem that the method of tumor brain metastasis model has not yet appeared.
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Embodiment 1
[0053] Apply the primary extracted SD rat brain microvascular endothelial cells (ECs) and brain astrocytes (As), and use the self-designed and produced microfluidic chip to establish a high-throughput in vitro blood-brain barrier model, such as Figure 1-2 shown.
Embodiment 3
[0057] Using the microfluidic chip described in Example 1, a single blood-brain barrier model was constructed with endothelial cells and astrocytes, and then exogenous tumor cells A549, MDA- For single-cell suspensions of MB-231 and M624 cells, stand the whole chip sideways for 10 minutes with the two-dimensional channel on the top and the three-dimensional channel on the bottom. After all the cells are attached to the surface of the blood-brain barrier model, place the chip flat and continue to culture . Take pictures and collect data every 24 hours, and observe the number of cells and the moving distance of cells that tumor cells break through the blood-brain barrier and transfer into the side channel, such as Figure 4 , 5 shown. Depend on Figure 4 , 5 It can be seen that neither the single astrocyte model group nor the single endothelial cell model group can well play a barrier role against the invasion of exogenous tumor cells into the brain.
Embodiment 4
[0059] Endothelial cells and astrocytes were co-cultured with exogenous tumor cells, and after continuous culture for 48 hours, the data were collected by taking pictures to observe the growth, proliferation and sphere formation among cells, as shown in Figure 6 shown. Depend on Figure 6 It can be seen that neither endothelial cells nor astrocytes can co-grow and proliferate with exogenous tumor cells, that is, they cannot coexist, indicating that endothelial cells and astrocytes cannot co-exist with exogenous tumor cells. The interaction explains why the blood-brain barrier has no barrier effect on exogenous tumor cells.
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