Gold-colloid test strip for detecting alpha-glucosan and detection method
A gold-labeled test paper and glucan technology, which is applied in measurement devices, instruments, scientific instruments, etc., can solve the problems that there is no semi-quantitative detection of α-glucan, no semi-quantitative detection, etc.
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Embodiment 1
[0031] Embodiment 1: the preparation method of α-glucan gold standard test strip
[0032] 1. Preparation of colloidal gold:
[0033] Colloidal gold was prepared by trisodium citrate reduction method. Take 50ml of 0.1mg / ml chloroauric acid solution, stir at 100r / min, heat to boiling, add 2ml of 10mg / ml sodium citrate solution, keep the original reaction temperature and stirring speed, continue to boil for 5min until the color of the solution just changes After turning to clear orange red, the reaction stopped. Cool and store in a brown bottle at 4°C in the dark. The particle size of the colloidal gold is 10-15nm as detected by the electron microscope.
[0034] 2. Preparation of α-glucan and BSA, OVA cross-linked product
[0035] Mix α-glucan DextranT-40 and BSA at a ratio of 1:1, dissolve in water to adjust to 6% (W / V), and freeze-dry. After being ground into powder, it was placed in an environment of 60 ° C, and a saturated KBr solution was placed at the bottom of t...
Embodiment 2
[0045] Embodiment 2: the using method of gold standard test paper of the present invention
[0046] Take 100ul of the sample and add it dropwise to the sample pad, start timing, and observe the test result after 15 minutes.
[0047] The sample concentrations represented by the three detection lines designed on this test strip are 5ng / ml, 20ng / ml, and 50ng / ml, and the interpretation standards of the results are as follows:
[0048] 1. When the quality control line is not displayed ( figure 1 ), the test result is invalid.
[0049] 2. When the quality control line and three detection lines appear ( figure 2 ), the measured concentration of α-glucan was less than 5ng / ml.
[0050] 3. When the quality control line and two detection lines appear ( image 3 ), the measured concentration of α-glucan was 5-20ng / ml.
[0051] 4. When a quality control line and a detection line appear ( Figure 4 ), the measured concentration of α-glucan was 20-50ng / ml.
[0052] 5. When there i...
Embodiment 3
[0053] Embodiment 3: sample detection example
[0054] Adopt the method of embodiment 2 to detect various samples, and detection result is as shown in table 1:
[0055] Table 1 Test results of various samples
[0056]
[0057] Wherein the test results of DextranT2000 and DextranT40 standard solution meet expectations. Glucose, starch, β-glucan and other samples will not interfere with the detection, showing that the test strip has good specificity.
[0058] The above examples show that the gold standard test paper for detecting α-glucan of the present invention has strong specificity, high sensitivity, simple, convenient and fast operation, does not require special equipment and professional training, and the results are clear and easy to distinguish, economical and practical.
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Abstract
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