Novel microbial enzyme rapid detection device based on PCR (polymerase chain reaction) technique

Abstract

The invention relates to a novel microbial enzyme rapid detection device based on a PCR (polymerase chain reaction) technique. The device comprises a PCR instrument, a display screen, an initial parameter setting panel, a reactant inlet, an upper equipment end cap, a cooling window, a microbial enzyme detection instrument and a data analysis computer, wherein the display screen is arranged on the upper surface of the PCR instrument, the initial parameter setting panel is arranged on the side surface of the display screen, the upper equipment end cap is arranged at the top of the PCR instrument, the reactant inlet is formed in the upper surface of the upper equipment end cap, the cooling window is formed in the side surface of the PCR instrument, a cooling fan is mounted in the cooling window, and the rear side of the PCR instrument is connected with the microbial enzyme detection instrument. The device has the benefits as follows: pollution caused by oscillation among microorganisms in the transfer process is greatly reduced; an intelligent temperature control system is adopted, a heating part and a refrigeration part operate separately, the reaction temperature can be quickly changed, and the reaction effect of a PCR is guaranteed.

Description

technical field [0001] The invention relates to the field of rapid detection equipment for microorganisms, in particular to a novel rapid detection device for microbial enzymes based on PCR technology. Background technique [0002] PCR (polymerase chain reaction) is a molecular biology technique used to amplify specific DNA fragments. It can be regarded as a special DNA replication outside the body. The biggest feature of PCR is that it can amplify a small amount of DNA Increase. Polymerase chain reaction is to use DNA denaturation at a high temperature of 95°C in vitro to become a single strand. At low temperature, primers and single strands are combined according to the principle of complementary base pairing, and then the temperature is adjusted to the optimum reaction temperature of DNA polymerase. DNA The polymerase synthesizes the complementary strand along the direction of the phosphate to the five-carbon sugar. Microbiological Rapid Detector is a portable rapid qua...

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Problems solved by technology

It is convenient and fast, and the result can be detected in three steps. The detection does not require professional training and has high specificity. The detection bottle can be used anytime and anywhere, and the specificity is as high as 99.999%. ;The detection is rapid, which is 2-5 times that of the traditional inspection method; it has high sensitivity and can accurately detect 1 target microorganism, but the existing PCR technology has the following disadvantages: cross-contamination of target sequences or amplified products; templates always contain impurities Lead to no amplification bands; the concentration of primers and Mg2+ concentration are too high to cause PCR amplification failure without amplified bands, and unstable temperature control, etc.

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