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An inoculation identification method capable of observing the symptoms of wheat black germ disease in time

An identification method and black germ disease technology are applied in the field of inoculation identification for observing the symptoms of wheat black germ disease in time, which can solve the problems of inability to observe symptoms in time and reduce the accuracy of identification results, so as to improve accuracy and stability, enhance Accuracy, the effect of ensuring the effect of inoculation

Active Publication Date: 2019-04-16
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is: in view of the inability to observe the symptoms in time by the direct spraying inoculation method, and the deficiency that the accuracy of the identification results is reduced due to the barrier between the bacteria and the grain due to the chaff, the present invention provides a method that can observe wheat black germ disease in time. Symptomatic inoculation identification method

Method used

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  • An inoculation identification method capable of observing the symptoms of wheat black germ disease in time
  • An inoculation identification method capable of observing the symptoms of wheat black germ disease in time
  • An inoculation identification method capable of observing the symptoms of wheat black germ disease in time

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Effect test

Embodiment 1

[0043] The present invention can timely observe the inoculation identification method of the symptoms of wheat black germ disease, the detailed steps of the inoculation identification method are as follows (the pathogenic bacteria adopted in this embodiment is Helminthromyces rotundum umbilicalis):

[0044] a. After the earing of the wheat potted in the greenhouse of the National Wheat Engineering Technology Research Center of Henan Agricultural University, use tweezers to remove the 3 spikelets on the upper and lower parts of the spikelets, and then use scissors to cut off the glumes of the remaining spikelets from the top by 1 / 2, and cut off the awns; then use a 25×17mm sulfuric acid paper bag to bag the ears of the sheared wheat to isolate miscellaneous bacteria (each bag covers 5 ears with the same development status);

[0045] b. Cultivation of pathogenic bacteria of wheat black germ disease: first, sterilize the PDA medium (sterilization temperature is 121°C, sterilizati...

Embodiment 2

[0051] Embodiment 2: basically the same as Embodiment 1, the difference is:

[0052] The method of the present invention can be used for the inoculation identification of multiple wheat black germ disease bacteria, and can observe black germ disease symptom in time, and concrete steps are basically the same as embodiment 1, and difference is:

[0053] In step b: the pathogenic bacteria of wheat black embryo disease used are Helminthosporium tritici root rot, Umbilium bermudagrass, Umbilium cricket and Phomomyces phlox.

[0054] The test results showed that the pathogenicity of 4 different pathogenic strains of wheat black embryo disease was different after inoculation, but the rate of black embryos identified by the shearing treatment was higher than that of the non-hulling treatment, and the symptoms were more obvious (details See shown in Fig. 3), show that the method of the present invention can be applicable to the inoculation identification of different wheat black embryo...

Embodiment 3

[0056] The present invention can observe the inoculation identification method of wheat black germ disease symptom in time, the present embodiment is under field planting conditions, inoculate four kinds of wheat black germ disease pathogenic bacteria to identify and observe wheat black germ disease symptom in time, its detailed steps are as follows :

[0057] a, panicle shearing treatment: after the earing of the wheat planted in Xingyang Breeding Field (Yulong Town, Xingyang City), use tweezers to remove the 3 spikelets on the upper and lower parts of the spikelets, and then use scissors to remove the glumes of the remaining spikelets. 1 / 2 of the shell was cut off from the top, and the awns were cut off; then, the ears of the sheared wheat were bagged with a 25×17 mm sulfuric acid paper bag to isolate miscellaneous bacteria (each bag covered 5 ears with the same development status), 3 bags per strain per variety;

[0058] b. Cultivation of pathogenic bacteria of wheat black...

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Abstract

The invention discloses an inoculation identification method capable of observing the symptoms of wheat black germ disease in time. First, after wheat earing, the wheat ears are subjected to hulling treatment and the husked wheat ears are subjected to miscellaneous bacteria isolation; the pathogenic bacteria of wheat black embryo disease are inoculated in a petri dish containing PDA medium for dark culture, Obtain the pathogenic bacteria colonies after cultivation; wash the pathogenic bacteria colonies obtained, filter, centrifuge, and adjust the spore concentration to obtain the spore suspension for later use; when the wheat grains are full of kernels, spray the spore suspension on the shell-cut ears Cover the ears with a plastic bag to keep the ears moist; observe the infection of the germs on the inoculated grains and the formation of black germ disease symptoms after inoculation; count the black germ rate when the wheat is mature, and use it as the identification result of wheat black germ disease. The method of the invention can not only observe the occurrence of symptoms in time, but also make the identification result more stable and reliable; and can timely and reasonably prevent and control the wheat according to the dissemination situation.

Description

technical field [0001] The invention belongs to the technical field of crop disease detection, and in particular relates to an inoculation identification method capable of observing symptoms of wheat black germ disease in time. Background technique [0002] Wheat black germ disease refers to symptoms such as black and brown in different shapes and degrees on the surface of wheat grains. This disease not only reduces the appearance quality of wheat grains, but also reduces the sales level of wheat in the market and affects farmers' income. It also reduces the germination rate of seeds. , affect the quality of flour, and bring serious harm to wheat production. [0003] The etiology of wheat black germ disease is complex, affected by pathogenic bacteria, environmental factors and variety characteristics, and its severity varies with the variety, location, year and control measures. The incidence rate reported by various countries is generally 0.3-66.7% (See: 1. Li Q Y, Qin Z, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/04A01G7/06
CPCA01G7/06
Inventor 李巧云姜玉梅牛吉山梁晓龙孙玉龙何瑞士朱欣欣于东艳
Owner HENAN AGRICULTURAL UNIVERSITY
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