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Setaria viridis-containing fusarium culture medium and preparation method thereof

A technology of foxtail grass and culture medium, which is applied in the field of fusarium culture medium containing foxtail grass and its preparation, and achieves the effects of simple formula, simple preparation method and easy operation

Active Publication Date: 2016-06-15
INST OF PLANT PROTECTION SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A high-quality, low-cost and easy-to-prepare medium is undoubtedly crucial for the research of biocontrol fungi, and it is also an important limiting condition that limits the low-cost utilization of biocontrol strains.

Method used

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  • Setaria viridis-containing fusarium culture medium and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation and culture effect comparison of different weed culture medium of embodiment 1

[0030] Take 50g green foxtail, manually chop the green foxtail until the stem and leaf fragments are less than 5 cm, boil the green foxtail stem and leaf for 30 minutes, filter the weed stem and leaf with gauze. Add 3 g of cornstarch, 1.0 g of magnesium sulfate heptahydrate, 1.0 g of potassium dihydrogen phosphate, 18 g of agar, and distilled water to 1000 ml to adjust the pH to 7.0 in the filtered foxtail juice. And set the volume to 1000ml. The solution after constant volume was kept at 121°C, 1.05×10 5 Sterilize in a sterilizing pot under Pa for 20 minutes, and set aside after sterilization. According to the composition, the medium was named Setaria cornstarch medium (SCA).

[0031] Replace the foxtail in the above culture with other weeds, and make the medium without changing the other steps. In this example, barnyardgrass, crabgrass, goosegrass, Amania miltiorrhiza,...

Embodiment 3

[0052] Embodiment 3 The cultivation effect comparison of Foxtail grass consumption in the substratum provided by the present invention

[0053]Based on the medium SCA provided by the present invention, gradient screening is carried out on the content of the material Foxtail in the medium, and the amount of materials used is reduced as much as possible on the premise of ensuring efficient cultivation of strains, reducing costs. Referring to the preparation method in Example 1, the Setaria gradient is set to six gradients of 0g, 25g, 50g, 100g, 200g, and 400g. With reference to the method mentioned in Example 1, the growth of Fusarium under different proportions was determined. The cultured strain was Fusarium graminearum.

[0054] Table 3 The spore production of Fusarium strain LD-1 Fusarium graminearum on different proportioning media

[0055] Foxtail (g)

0

25

50

100

200

Sporulation (10 9 )

19.6±2.3 c

203.7±9.6 b

396.3±22.4 ...

Embodiment 4

[0058] The growth difference of different Fusarium bacterial strains in the SCA medium of embodiment 4

[0059] Configure the culture medium provided by the present invention with reference to the method of Example 1, and verify various Fusariums (LD-1: Fusarium graminearum; LD-2: Fusarium moniliforme; LD-3: Oxysporium oxysporum) with reference to the method of Example 3 Fusarium; LD-4: Fusarium chlamydosporum) growth rate in the medium provided by the present invention.

[0060] The growth rate of different fusarium strains of table 4 on the medium of the present invention

[0061] strain

LD-1

LD-2

LD-3

LD-4

Colony diameter (cm)

6.3±0.7 a

6.6±0.4 a

6.3±0.5 a

6.5±0.2 a

Sporulation (10 9 )

409.7±16.8 a

357.2±31.2 ab

416.8±28.7 a

376.5±19.7 ab

[0062] Note: The values ​​in the table are mean ± standard error (n=3), different letters after the data in the same row indicate significant differences at ...

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Abstract

The invention relates to a setaria viridis-containing fusarium culture medium and a preparation method thereof. A formula of preparing 1000ml of the culture medium comprises the following components: 50-200g of setaria viridis, 3g of corn starch, 1.0g of magnesium sulfate heptahydrate, 1.0g of monopotassium phosphate and 15-20g of agar, wherein distilled water is added till being 1000ml, and the pH value is adjusted to 6.0-8.0. The culture medium takes weed setaria viridis which is normal in fields as a main material, and a small amount of corn starch, magnesium sulfate heptahydrate and monopotassium phosphate are added as an assistant carbon source and inorganic salt components. The culture medium provided by the invention is simple in formula and very easy to prepare without special equipment; by adopting the culture medium, rapid spore production and culture for a plurality of fusaria can be achieved, and the fusaria can grow rapidly with very large spore production amounts.

Description

technical field [0001] The invention belongs to the technical field of microorganism cultivation, and in particular relates to a Fusarium culture medium containing Setaria japonicus and a preparation method thereof. Background technique [0002] At present, there are few studies on the recycling of field weeds, which has caused a lot of waste of biomass resources. For example, Setaria is a common weed in dryland crops, widely distributed in corn planting areas in many provinces and cities in my country. The growth period of foxtail grass is relatively consistent with that of corresponding crops, and its root system is well developed, with a strong ability to absorb water and nutrients, and it is easy to compete with field crops for conditions such as water, nutrients, light and space, and its growth ability is very strong. At present, chemical control methods are used for the troublesome weeds in farmland, and there is a lack of recycling methods. [0003] Fusarium is a co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/77
CPCC12N1/14
Inventor 李健李美高兴祥房锋
Owner INST OF PLANT PROTECTION SHANDONG ACAD OF AGRI SCI