Detection method of Sudan Red I
A detection method, Sudan red technology, applied in 1 field, can solve the problems of long detection time, high detection cost, inconvenient actual analysis, etc., and achieve the effect of simple and convenient operation, low detection limit and simple operation
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Embodiment 1
[0035] 1,8-naphthalenediamine / Cu 2+ Fluorescent test of compound solution
[0036] to a final concentration of 1×10 -6 moL -1 The 1,8-naphthalene diamine solution was added to the final concentration of 0, 1 × 10 -6 moL -1 , 1.5×10 -6 moL -1 , 2.25×10 -6 moL-1 , 3.0×10 -6 moL -1 , 3.75×10 -6 moL -1 , 4×10 -6 moL -1 , 5×10 -6 moL -1 , 7.0×10 -6 moL -1 , 7.5×10 -6 moL -1 Cu 2+ solution, respectively test the fluorescence spectrum of each composite solution system.
[0037] Test results such as figure 1 As shown, the 1,8-naphthalene diamine solution has a strong fluorescence intensity, when adding Cu 2+ After that, the fluorescence intensity of 1,8-naphthalenediamine increased with Cu 2+ gradually decreased with the increase of the concentration, indicating that the Cu 2+ It has a quenching effect on the fluorescence of 1,8-naphthalenediamine.
[0038] 1,8-naphthalenediamine and Cu 2+ The concentration can not only affect the fluorescence intensity of the...
Embodiment 2
[0040] A detection method for Sudan Red I, said method comprising the following steps:
[0041] A. Configure multiple groups of 1,8-naphthalenediamine / Cu 2+ Composite solution system: Take 1mL concentration to be 1×10 -5 moL -1 The concentration of 1,8-naphthalene diamine aqueous solution with 1mL is 3.75×10 -5 moL -1 Cu 2+ Add the aqueous solution into a stoppered colorimetric tube and react for 20 minutes;
[0042] B. Add 1mL to the above solution successively with a concentration of 0~7.5×10 -6 moL -1 Sudan red I aqueous solution, and add 1mL of PEPES buffer solution with pH 6.7, and finally dilute to 10mL with deionized water, stir the mixture for 70min and then measure its fluorescence intensity;
[0043] The final concentrations of the Sudan I aqueous solution are respectively 0, 5 × 10 -7 moL -1 , 12×10 -7 moL -1 , 17×10 -7 moL -1 , 23×10 -7 moL -1 , 28×10 -7 moL -1 , 34×10 -7 moL -1 , 40×10 -7 moL -1 , 46×10 -7 moL -1 .
[0044] Experimental res...
Embodiment 3
[0047] 1,8-naphthalenediamine / Cu 2+ Optimization of the system for the detection method of Sudan Red I
[0048] The pH value is a very important parameter that can affect the detection of the sensing system. In the experiment, a HEPES buffer solution with a pH value of 1.75-9.5 was selected to adjust the pH of the system. When the fluorescence recovery efficiency of the system (F-F 0 ) / F 0 (where F 0 and F are 1,8-naphthalenediamine / Cu 2+ Fluorescence intensity and 1,8-naphthalenediamine / Cu of the composite system at 440nm wavelength 2+ The pH when the complex is added to the maximum pH at 440nm wavelength (fluorescence intensity at 440nm wavelength place when Sudan Red I is added) is the optimal pH value, and the results are as follows Figure 5 shown.
[0049] from Figure 5 It can be seen from the figure that as the pH value increases, the fluorescence recovery efficiency of the system first increases and then decreases, and the fluorescence recovery efficiency of th...
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