A method for the simultaneous determination of tobacco-specific n-nitrosamines and polycyclic aromatic hydrocarbons in mainstream cigarette smoke
A technology for cigarette mainstream smoke and polycyclic aromatic hydrocarbons, which is applied in the field of chemical testing of cigarette smoke, can solve the problems of instrument analysis conditions and parameter differences, and achieve the effects of simple operation, high degree of automation, and reduced testing costs
Active Publication Date: 2017-10-31
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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However, there are great differences in the instrument analysis conditions and parameters (mobile phase, gel chromatography collection time, mass spectrometry parameters, etc.) of the two methods.
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Embodiment 1
[0019] A method for determining tobacco-specific N-nitrosamines and polycyclic aromatic hydrocarbons in mainstream cigarette smoke. The specific steps are as follows:
[0020] The first step is to prepare the standard series of solutions: use ethyl acetate and acetone with a volume ratio of 1:1 as solvents, four kinds of tobacco-specific N-nitrosamines (NAB, NAT, NNN and NNK) and three kinds of polycyclic aromatic hydrocarbons (BaP, BaA and Chry) standard products are solutes, NAB-d4, NAT-d4, NNN-d4, NNK-d4 and BaP-d12 are internal standards, prepare different concentrations of standard series solutions containing internal standards;
[0021] The second step is the preparation of sample solution: the balanced and screened cigarettes are smoked with a linear smoking machine in accordance with the standard conditions specified in GB / T19609-2004 and ISO 4387:2000, and 5 cigarettes are smoked in each experiment. Place the filter with the total particulate matter of mainstream smoke in ...
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The invention belongs to the technical field of chemical tests on cigarette smoke and particularly discloses a method for determining tobacco-specific N-nitrosamine and polycyclic aromatic hydrocarbons in mainstream smoke of cigarettes simultaneously. According to the method, a smoking machine smokes cigarettes, a Cambridge filter traps the mainstream smoke of the cigarettes, the filter is filtered by solutions containing interior labels, and an extraction solution is directly detected by an on-line gel chromatography-gas chromatography-tandem mass spectrometer after being filtered. Compared with a standard method, the method has the advantages that two compounds are determined together and the test efficiency is improved; all that is need for detection is to filter the smoke extraction solution, so that the method is convenient to operate, quick and efficient; the on-line gel chromatography-gas chromatography-tandem mass spectrometer is used for detection, and thus the automation degree is high and organic solvent consumption is low.
Description
Technical field [0001] The invention belongs to the technical field of cigarette smoke chemical inspection, and specifically relates to a method for simultaneously determining tobacco-specific N-nitrosamines and polycyclic aromatic hydrocarbons in mainstream cigarette smoke. It uses online gel chromatography-gas chromatography-tandem mass spectrometry. Instrument for testing. Background technique [0002] Polycyclic aromatic hydrocarbons are the products of incomplete combustion of tobacco under high temperature and hypoxic conditions. At present, about 30 carcinogenic polycyclic aromatic hydrocarbons have been identified in mainstream cigarette smoke. In 1967, Hoffmann et al. proved polycyclic aromatic hydrocarbons through toxicological experiments. The carcinogenicity of aromatic hydrocarbons, subsequent medical reports published confirm that benzo[a]pyrene is the most important carcinogen among polycyclic aromatic hydrocarbons. Tobacco-specific N-nitrosamines mainly refer to ...
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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/027
Inventor 罗彦波陈小静姜兴益李翔宇张洪非李雪刘洋朱风鹏庞永强
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT