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High-performance human urine microalbumin detection kit

A microalbumin and detection kit technology, applied in the biological field, can solve the problems of low linear range and insufficient antigen excess, and achieve the effects of saving manpower and reagents, saving patient expenses, and high reliability

Inactive Publication Date: 2016-06-15
BYRON DIAGNOSTICS SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Microalbuminuria is also a sign of changes in the entire vascular system, and can be considered a "window" of arterial disease, because it is an early indicator of changes in the kidney and cardiovascular system, so it is urgent to develop a method that can detect human urine microalbumin However, the current kits for microalbumin detection on the market generally have a low linear range and insufficient antigen excess.

Method used

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  • High-performance human urine microalbumin detection kit
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  • High-performance human urine microalbumin detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037]The particle size of the latex particles in this example is 230nm, and 17ml of the rabbit anti-human albumin polyclonal antibody produced by Dako Company (article number: Q0328) is labeled per liter of reagent 2, and the most conventional phosphate buffer in latex immunoturbidimetry is used as reagent 1 , using the most common parameters of the automatic analyzer Reagent 1: Reagent 2 ratio 250 μl: 50 μl, the sample was pre-diluted 60 times and then added 3 μl of the diluted sample to the reaction system (that is, the ratio of sample to antibody was 1:17).

[0038] Using Hitachi7170 automatic biochemical analyzer, the wavelength is 660nm single wavelength, and the two-point endpoint method is calculated at 18-34 reading points. The data are as follows:

[0039] Table 1 Calibration data

[0040] Concentration of calibrator ㎎ / L

[0041] Table 2 Linear Dilution

[0042] Dilution ratio

[0043] Specific as figure 1 And as shown in Table 2, a) linear corr...

Embodiment 2

[0050] The particle size of the latex particles in this example is 150nm, and 12ml of the rabbit anti-human albumin polyclonal antibody (article number: Q0328) produced by Dako Company is labeled per liter of reagent 2, and the most conventional phosphate buffer in latex immunoturbidimetry is used as reagent 1 , using the most common parameters of the automatic analyzer Reagent 1: Reagent 2 ratio 250μl: 50μl, the sample was pre-diluted 60 times and then added 3μl of the diluted sample to the reaction system (that is, the ratio of sample to antibody was 1:12).

[0051] Hitachi7170 automatic biochemical analyzer was used, the wavelength was 660nm single wavelength, and the two-point endpoint method was used to calculate 18-34 reading points. The data are as follows.

[0052] Table 5 Calibration data

[0053] Concentration of calibrator ㎎ / L

[0054] Table 6 Linear Dilution

[0055]

[0056]

[0057] Specific as figure 2 And as shown in Table 6, a) linear correl...

Embodiment 3

[0064] The particle size of the latex particles in this example is 230nm, and 12ml of the rabbit anti-human albumin polyclonal antibody produced by Dako Company (article number: Q0328) is labeled per liter of reagent 2, and the most conventional phosphate buffer in latex immunoturbidimetry is used as reagent 1 , using the most common parameters of the automatic analyzer Reagent 1: Reagent 2 ratio 250μl: 50μl, the sample was pre-diluted 60 times and then added 3μl of the diluted sample to the reaction system (that is, the ratio of sample to antibody was 1:12).

[0065] Hitachi7170 automatic biochemical analyzer was used, the wavelength was 660nm single wavelength, and the two-point endpoint method was used to calculate 18-34 reading points. The data are as follows.

[0066] Table 9 Calibration data

[0067] Concentration of calibrator ㎎ / L

[0068] Table 10 Linear Dilution

[0069]

[0070]

[0071] Specific as image 3 And as shown in Table 10, a) linear corre...

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Abstract

The invention discloses a high-performance human urine microalbumin detection kit. The kit of the present invention includes reagent 2, which is a solution of latex particles labeled with rabbit anti-human albumin polyclonal antibody; wherein, the particle diameter of the latex particles ranges from 150nm to 350nm, and the antibody in each liter of reagent 2 The dosage is 12ml~22ml. The invention can replace imported reagents, save patients' expense, and improve detection efficiency at the same time.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a high-performance human urine microalbumin detection kit. Background technique [0002] Under normal human metabolism, there is very little albumin in urine, specifically no more than 20mg per liter of urine albumin (<20mg / L), so it is called microalbumin. If the microalbuminuria in the urine is found to be in the range of 20mg / L-200mg / L after the physical examination, it belongs to microalbuminuria. If the patient can repair the nephron and reverse the fibrosis treatment, the renal microalbuminia can be completely repaired. Ball, eliminate proteinuria, urine routine urine protein showed negative (-) or (+-). And when the trace albumin in urine exceeds 200mg / L, it should be noticed. At this time, it proves that a large amount of albumin has leaked out in patients with kidney disease, and hypoalbuminemia may occur. The development of kidney disease is only one step away from the ...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6827
Inventor 骆春梅王钊
Owner BYRON DIAGNOSTICS SHANGHAI
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