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Method for increasing yield of chlorella through two-round bacterium adding co-culture and method for preparing biological feed

A technology of biological feed and co-cultivation, applied in the field of preparation of biological feed, achieves the effect of low cost, significant benefit and simple operation

Inactive Publication Date: 2016-06-22
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Because chlorella has chloroplasts like plant cells and can perform photosynthesis, its biochemical metabolic characteristics are similar to those of plants. In theory, plant endophytes that have metabolic regulation effects on plants can also affect the growth and metabolism of chlorella activities, but so far there is no technical research and application of plant endophytes including rice endophytes to promote the growth of chlorella and the synthesis and accumulation of biochemical components

Method used

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  • Method for increasing yield of chlorella through two-round bacterium adding co-culture and method for preparing biological feed

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Effect test

Embodiment 1

[0031] 1) The rice endophytic Pantoea strains were purchased from the China Agricultural Microbiology Collection Center (ACCC), and the code is 10454. After the strains were activated by conventional methods, they were inoculated into a Erlenmeyer flask containing 10mL of LB liquid medium for expansion. For expansion culture, the bacteria liquid in the logarithmic growth phase after the expansion culture for 16 hours is inoculated according to the volume ratio of bacteria liquid: liquid LB medium = 1:10, and then the second expansion culture is carried out. The culture conditions are 37°C, 200rpm constant temperature shaker dark culture. The second expansion culture 10h to OD 600 =1 is used as the seed cell of the co-cultivation of bacteria and algae. According to the growth curve, the growth of the bacteria is in the logarithmic phase. The effective number of viable bacteria (CFU, Colony-FormingUnits) in the culture solution is measured by the conventional plate counting meth...

Embodiment 2

[0037] 1) The rice endophytic Pantoea strains were purchased from the China Agricultural Microbiology Collection Center (ACCC), and the code is 10454. After the strains were activated by conventional methods, they were inoculated into a Erlenmeyer flask containing 10mL of LB liquid medium for expansion. For expansion culture, the bacteria liquid in the logarithmic growth phase after the expansion culture for 16 hours is inoculated according to the volume ratio of bacteria liquid: liquid LB medium = 1:10, and then the second expansion culture is carried out. The culture conditions are 37°C, 200rpm constant temperature shaker dark culture. The second expansion culture 10h to OD 600 =1 is used as the seed cell of the co-cultivation of bacteria and algae. According to the growth curve, the growth of the bacteria is in the logarithmic phase. The effective number of viable bacteria (CFU, Colony-FormingUnits) in the culture solution is measured by the conventional plate counting meth...

Embodiment 3

[0043] 1) The rice endophytic Pantoea strains were purchased from the China Agricultural Microbiology Collection Center (ACCC), and the code is 10454. After the strains were activated by conventional methods, they were inoculated into a Erlenmeyer flask containing 10mL of LB liquid medium for expansion. For expansion culture, the bacteria liquid in the logarithmic growth phase after the expansion culture for 16 hours is inoculated according to the volume ratio of bacteria liquid: liquid LB medium = 1:10, and then the second expansion culture is carried out. The culture conditions are 37°C, 200rpm constant temperature shaker dark culture. Continuous expansion was carried out three times, and the culture conditions were 37°C, 200rpm constant temperature shaker and dark culture. The third expansion culture 10h to OD 600 =1 is used as the seed cell of the co-cultivation of bacteria and algae. According to the growth curve, the growth of the bacteria is in the logarithmic phase. T...

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Abstract

The invention discloses a method for increasing the yield of chlorella through two-round bacterium adding co-culture and a method for preparing biological feed. The method for increasing the yield of the chlorella through two-round bacillus-alga adding co-culture comprises the steps that 1, rice endophytic bacteria added at the first time and the chlorella are subjected to first-round bacterium-chlorella co-culture under illumination, and a first-round bacterium-chlorella co-culture solution is obtained after culture is finished; 2, rice endophytic bacteria added at the second time are replenished into the first-round bacterium-chlorella co-culture solution, second-round bacterium-chlorella co-culture is carried out under illumination, and a second-round bacterium-chlorella co-culture solution is obtained after culture is finished; 3, the second-round bacterium-chlorella co-culture solution is subjected to cell separation, and chlorella cell sediment and a supernatant bacterium solution containing the rice endophytic bacteria are obtained. According to the method, the chlorella pyrenoidosa living cell sediment can serve as a biological matrix for degradation of azo dyes in water and other sewage purifying treatment after being washed and resuspended, dried chlorella cells can be obtained after the chlorella pyrenoidosa living cell sediment is dried, and the dried chlorella cells serve as raw materials for preparing the feed and extracting grease, protein, pigments, growth factors and other related biologics.

Description

technical field [0001] The invention relates to the field of microorganisms and biotechnology, in particular to a method for increasing the yield of chlorella by two rounds of co-cultivation with bacteria and a method for preparing biological feed. Background technique [0002] Chlorella is a single-celled green algae, which has strong environmental adaptability and is easy to cultivate on a large scale. The cells contain rich nutrients and are widely used in health food, feed, food additives, finely processed products and as raw materials for pharmaceutical preparations , and its development and utilization have attracted widespread attention at home and abroad. The common types of chlorella in my country include Chlorella pyrenoidosa, Chlorella ellipsoides, Chlorella vulgaris, etc. Among them, Chlorella pyrenoidosa algae powder crude protein kernel has a high content of essential amino acids, and is an excellent feed protein source ( Li Guoping. Analysis of amino acid cont...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/12A23K10/18C12R1/89C12R1/01
CPCC12N1/12C12N1/20
Inventor 赵艳
Owner ZHEJIANG GONGSHANG UNIVERSITY
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