A culture method capable of improving the differentiation phenotype and function of hepatocytes in vitro
A culture method and in vitro culture technology, applied in cell culture supports/coatings, hepatocytes, artificial cell constructs, etc., can solve problems such as the reduction of enzyme functional activity, and achieve the effect of improving material transfer
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[0018] The specific implementation manner of the present invention will be described below with reference to the accompanying drawings. Such as Figure 1 to Figure 7 Shown:
[0019] Preparation of porous silk protein scaffold:
[0020] First prepare a silk fibroin solution with a concentration of 6-7%, and prepare a porous silk fibroin scaffold by salting out-drying method. The pore size of the scaffold is controlled by the size of the salt particles to be 500-600 μm, and fully soaked in deionized water after drying. Then a three-dimensional scaffold with a diameter of 5 mm and a thickness of 3 mm (that is, a porous silk protein scaffold) was prepared using a standard puncher. The porous silk protein scaffold needs to be sterilized by high temperature and high pressure before inoculating cells, and soaked in the medium for pre-equilibration for 12 h.
[0021] Isolation and culture of primary rat hepatocytes:
[0022] After rats were anesthetized, two-step perfusion with co...
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