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Antrodia camphorata mycelium active substance and composition for protecting nerve cells

A technology of active substances and mycelium, which is applied in the direction of drug combination, nervous system diseases, medical raw materials derived from fungi, etc., can solve the problems affecting the composition of active ingredients, the difficulty of obtaining camphor wood, and the unfixed source of basswood matrix, etc., to achieve Reduces the risk of contamination and suppresses neurotoxic effects

Active Publication Date: 2016-06-29
GRAPE KING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] 1. Antrodia camphorata is a biological species residing in camphor wood, which belongs to the first-class conservation wood species, and hollow camphor wood is not easy to obtain;
[0017] 2. The source of the basswood matrix used to cultivate Antrodia camphorata is not fixed, and the change of the matrix also affects the composition of its active ingredients

Method used

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  • Antrodia camphorata mycelium active substance and composition for protecting nerve cells
  • Antrodia camphorata mycelium active substance and composition for protecting nerve cells
  • Antrodia camphorata mycelium active substance and composition for protecting nerve cells

Examples

Experimental program
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Effect test

Embodiment 1

[0038] (1) The source of the organism

[0039] The bacterial strain A. camphorataCCRC-35396 used in the present invention was purchased from the Food Industry Development Institute.

[0040] (2) Fermentation of organisms

[0041] Inoculate the CCRC-35396 strain into 1L medium (1.0% glucose, 0.5% soybean powder, 0.5% digested protein, 0.01% MgSO 4 , 0.01% defoamer (KM-72antifoam), pH 4.0) in a 2-L Hinton flask, rotate and shake at 28°C for ten days. The cultured Antrodia camphorata mycelium was transferred to a 200-L fermenter containing 120L of the above medium in a sterile room, and fermented at 28°C for twelve days.

[0042] (3) Extraction of organisms

[0043] Freeze-dry the mycelium fermentation liquid obtained after fermentation, extract the freeze-dried mycelium freeze-dried powder with 100% ethanol of 10-30 times its volume, and carry out shaking extraction with a shaker flask, at 10-250rpm Extract by shaking at 15-25°C for 24 hours, repeat the extraction several ti...

Embodiment 2

[0047] Analysis of nerve cell toxicity damage and protection of nerve cells of active substances of Antrodia camphorata mycelium described in Example 1.

[0048] 1. Antagonize Amyloidβ (25-35) Induced primary cortical neuron toxicity

[0049] Take out the cerebral cortex (primarybraincortex) of the Sprague-Dawley rat that gives birth to one day, utilize 0.5mg / ml papaya enzyme (papain) to break up the cortical tissue and filter and lay before using polylysine solution (poly-l -lysine) on the 24-well flat-bottomed tissue culture dish with a density of 5000cells / ml-well; after six days, each cell was properly pasted on it; finally these cells were tested with samples (Antrodia camphorata described in Example 1 Mycelia active substances) to test. The test samples were dissolved in DMSO with concentrations of 10, 20, 40 and 50 mM, respectively, using dimethyl sulfoxide (DMSO) as a solvent. The concentration of DMSO in the medium (medium) was maintained at no more than 1 μl / ml to...

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Abstract

The invention discloses an antrodia camphorata mycelium active substance and a composition for protecting nerve cells. Under the noncytotoxic concentrations, i.e., 50 [mu]M, the antrodia camphorata mycelium active substance can inhibit the cytotoxicity caused by 10 [mu]M of amyloid protein (beta-amyloid (25-35)), and also can inhibit the cell apoptosis due to the mitochondrion breakage caused by 1-methyl-4-phenylpyridine (MPP<+>), and therefore, the antrodia camphorata mycelium active substance has the potential of preventing neurodegenerative diseases.

Description

technical field [0001] The invention relates to mycelia active substances, in particular to antrodia camphorata mycelium active substances and a composition for protecting nerve cells. Background technique [0002] Neurodegenerative Diseases: [0003] Neurodegenerative diseases are symptoms in which neurons in the brain and central nervous system gradually lose function. The brain and spinal cord are composed of various neurons, each responsible for different functions, such as controlling movement, processing and receiving information, and making judgments. The hippocampus in the brain is mainly the hub responsible for learning and memory. Damage to nerve cells in the hippocampus can lead to dementia and learning defects. Neurodegenerative diseases can result from the deterioration of neurons or the loss of function of the myelin sheath that transmits messages over time. Because the nerve cells in the brain and spinal cord are not easy to regenerate, as long as they are ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/07A61P39/02A61P25/00
Inventor 陈劲初许胜杰陈彦博林定威
Owner GRAPE KING
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