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Use of diarylheptanoid compound in preparation of drug or food for preventing and treating psoriasis

A technology of diphenylheptane and compounds, applied in the field of application of diphenylheptane compounds in the preparation of medicines or food for the prevention and treatment of psoriasis

Inactive Publication Date: 2016-07-06
GUANGDONG HOSPITAL OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no relevant report on the inhibition of the proliferation of human T-cell lymphoma cell line HH cells and human immortalized epidermal keratinogenic cell line HaCaT cells involved in the present invention

Method used

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  • Use of diarylheptanoid compound in preparation of drug or food for preventing and treating psoriasis
  • Use of diarylheptanoid compound in preparation of drug or food for preventing and treating psoriasis
  • Use of diarylheptanoid compound in preparation of drug or food for preventing and treating psoriasis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Extraction and preparation of diphenylheptane compounds of the present invention

[0030] Take dried Guangxi zedoary turmeric rhizomes and extract them with 95% ethanol (ethanol: water 95:5, v / v). The extracts are separated by HP-20 macroporous adsorption resin column chromatography, followed by water, 30% ethanol, 90-100% Ethanol gradient elution (12L for each gradient eluate), collect each eluate, and concentrate under reduced pressure to obtain water elution part, 30v / v% ethanol elution part, and 90-100v / v% ethanol elution Part: Take the 90~100v / v% ethanol elution part, separate it by silica gel column chromatography, use chloroform-methanol gradient elution (each gradient eluent volume is 12L), collect the fraction E with the chloroform-methanol ratio of 9:1 , Fraction E was separated by ODS column chromatography, eluted with a methanol-water gradient, and fraction E-5 with a methanol-water ratio of 6:4 was collected. Fraction E-5 was separated by preparativ...

Embodiment 2

[0031] Example 2: Detection of the diphenylheptane compound of the present invention inhibiting the proliferation of human T cell lymphoma cell line HH cells in vitro

[0032] Collect HH ​​cells in logarithmic growth phase by centrifugation at 1600 rpm for 3 min, and resuspend them in a high-sugar medium containing 10% FBSRPMI1640; add 100 μL of HH cell suspension to each well of a 96-well plate to a final concentration of 2×10 4 Cells per well. The compound 1 and compound 2 isolated in Example 1 were dissolved in DMSO and prepared into a storage solution with a final concentration of 10 mM; when used, 10 μL of the storage solution was added to 90 μL of RPMI1640 medium and diluted into a 1 mM use solution; At the same time, 10 μL of DMSO was added to 90 μL of RPMI1640 medium as a negative control solution. Add 0, 1, 2, 2.5, 3, 3.5, 5, 10 μL of the use solution to a 96-well plate to make a concentration gradient of 0, 10, 20, 25, 30, 35, 50, 100 μM; at the same time, negative The...

Embodiment 3

[0035] Example 3: In vitro inhibition of the compound of the present invention on the proliferation of human immortalized epidermal keratinocyte cell line HaCaT cells

[0036] Trypsin-EDTA was used to digest HaCaT cells in the logarithmic growth phase; collected by centrifugation at 1600 rpm for 3 min, and resuspended in DMED high-sugar medium containing 10% FBS; 100 μL of cell suspension was added to each well of a 96-well plate, The final concentration is 5000 cells per well; 37℃, 5% CO 2 Incubate in an incubator for 12 hours; according to the above method, add different volumes of use solution to a 96-well plate to make a concentration gradient of 0, 10, 20, 25, 30, 35, 50, 100 μM; at the same time, add the corresponding negative control group Volume of liquid used in the negative control group. 37°C, 5% CO 2 Incubate in an incubator for 24 hours, add 10 μL of CCK-8 to each well, incubate in the incubator for 4 hours in the dark, measure the absorbance at 450 nm with a micropl...

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Abstract

The invention belongs to the technical field of medicine and discloses the application of diphenylheptane compounds in the preparation of medicines or food for preventing and treating psoriasis. The diphenylheptane compound has the structure shown in formula I: Wherein, the 4-5 position of the diphenylheptane compound is a single bond or a double bond, and the 6-7 position is a single bond or a double bond; R 1 is double bond oxygen or acetoxy; R 2 is hydrogen, hydroxy or methoxy. The diphenylheptane compound of the invention has inhibitory effect on the proliferation of human skin T cell lymphoma cell line HH cells and human immortalized epidermal keratinogenic cell line HaCaT cells, and can be applied to the preparation of medicines or food for preventing and treating psoriasis.

Description

Technical field [0001] The invention belongs to the technical field of medicine, and particularly relates to the application of diphenylheptane compounds in the preparation of medicines or foods for preventing and treating psoriasis. Background technique [0002] Psoriasis is a common and frequently-occurring disease in dermatology. Its causes are complex and recurrent, and it has always been a medical problem that plagues the world. Its histopathological characteristics are mainly manifested as abnormal proliferation and differentiation of epidermal keratinocytes, accompanied by infiltration of skin inflammatory cells. At present, there are four main methods of western medicine to treat the disease: topical treatment, such as steroid hormones, tacrolimus and vitamin D 3 Derivatives, etc.; systemic drug therapy, such as tretinoin, methotrexate and cyclosporine, etc.; physical therapy, such as narrow-band ultraviolet radiation therapy; biological agents, such as etanercept and alf...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/12A61K31/222A61P17/06A23L1/30
Inventor 卢传坚陈少丹赵瑞芝高进涛
Owner GUANGDONG HOSPITAL OF TRADITIONAL CHINESE MEDICINE
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