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Preparation method of xenogenic acellular dermal matrix

A technology of acellular dermis and dermal matrix, which is applied in the field of preparation of heterogeneous acellular dermal matrix, can solve the problems of unfavorable adhesion and growth of epithelial cells, incomplete removal of cell components, and destructive basement membrane structure, etc., and achieve good social benefits and economic benefits, reduced scarring, and good technical results

Active Publication Date: 2016-07-06
王忠新
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the preparation of the existing heterogeneous acellular dermal matrix is ​​relatively mature, there are still some problems in its preparation and application, such as: incomplete removal of cell components, high antigenicity, poor tissue compatibility, and easy failure of skin grafting; It is very destructive to the basement membrane structure, which is not conducive to the adhesion and growth of epithelial cells; the preparation process is complicated and the processing time is too long; the preparation cost is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for preparing a heterogeneous acellular dermal matrix, specifically comprising the following steps:

[0021] 1) Take the skin: choose Parmesan pigskin as the skin source, depilate and disinfect the skin-taking area, cut 0.3mm slices of faulty skin with epidermis and dermis with a skin knife, cut it into the required size, and use Soak in 1‰ benzalkonium chloride solution for 30 minutes, degrease and sterilize;

[0022] 2) Exfoliating the epidermis: soaking the split skin obtained in step 1) with 0.25% trypsin solution at a constant temperature of 4°C for 23 hours, peeling off the epidermis, and washing the remaining part with sterile PBS solution for 4 times;

[0023] 3) Decellularization treatment: the dermal matrix obtained in step 2) was ultrasonically cleaned with 0.5% TritonX-100 solution at room temperature for 5 minutes, shaken for 6 hours, ultrasonically cleaned for 5 minutes again, shaken for 4 hours, and finally transferred to sterile PBS Shake in th...

Embodiment 2

[0028] A method for preparing a heterogeneous acellular dermal matrix, specifically comprising the following steps:

[0029] 1) Take the skin: choose Parmesan pigskin as the skin source, depilate and disinfect the skin-taking area, cut 0.3mm slices of faulty skin with epidermis and dermis with a skin knife, cut it into the required size, and use Soak in 1‰ benzalkonium chloride solution for 20 minutes, degrease and sterilize;

[0030] 2) Exfoliate the epidermis: soak the split skin piece obtained in step 1) with 0.25% trypsin solution at a constant temperature of 4° C. for 22 hours, peel off the epidermis, and wash the remaining part with sterile PBS solution for 5 times;

[0031] 3) Decellularization treatment: the dermal matrix obtained in step 2) was ultrasonically cleaned with 0.5% TritonX-100 solution at room temperature for 7 minutes, shaken on a shaker for 6 hours, ultrasonically washed again for 7 minutes, shaken on a shaker for 4 hours, and finally transferred to ster...

Embodiment 3

[0036] A method for preparing a heterogeneous acellular dermal matrix, specifically comprising the following steps:

[0037] 1) Take the skin: choose Parmesan pigskin as the skin source, depilate and disinfect the skin-taking area, cut 0.5mm slices of faulty skin with epidermis and dermis with a skin knife, cut it into the required size, and use Soak in 1‰ benzalkonium chloride solution for 40 minutes, degrease and sterilize;

[0038] 2) Exfoliate the epidermis: soak the split skin piece obtained in step 1) with 0.25% trypsin solution at a constant temperature of 4°C for 24 hours, peel off the epidermis, and wash the remaining part with sterile PBS solution for 3 times;

[0039]3) Decellularization treatment: the dermal matrix obtained in step 2) was ultrasonically cleaned with 0.5% TritonX-100 solution at room temperature for 3 minutes, shaken for 5 hours, ultrasonically cleaned for 3 minutes again, shaken for 3 hours, and finally transferred to sterile PBS Shake in the solu...

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Abstract

The invention discloses a preparation method of a xenogenic acellular dermal matrix. The method specifically includes the following steps of taking dermis, removing epidermal layers, conducting acellular disposal, punching holes through lasers, and conducting sterilizing. By means of the method, cell components are removed thoroughly, antigenicity is low, the dermal collagen structure is kept, histocompatibility is high, the time of the whole preparation process is only about 60 hours, disposal time is short, disposal cost is low, and good social benefits and economic benefits are achieved.

Description

technical field [0001] The invention relates to the technical field of tissue engineering, in particular to a method for preparing a heterogeneous acellular dermal matrix. Background technique [0002] Skin defects caused by burns, trauma, epithelial cancer resection, and skin diseases often leave scar hyperplasia and contracture deformities, which seriously affect the appearance and function. At present, in addition to autologous skin and skin flaps, the repair materials used to repair wounds mainly include allogeneic skin, heterogeneous skin, and various synthetic wound coverings. In the treatment of patients with extensive burns, the patient’s own skin source is limited, so these skin substitutes need to be used; the source of allogeneic skin is limited, there is a risk of infectious diseases, and it is restricted by ethics; Poor infectivity and expensive. In contrast, after acellular degeneration treatment, xenograft skin has reduced antigenicity, wide sources, low pri...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/60A61L27/56
CPCA61L27/362A61L27/3687A61L27/56A61L27/60A61L2430/40
Inventor 谢春晖于家傲白杨石凯金正花赵景春洪雷张喜薛岩张楠陈欣欣高欣欣
Owner 王忠新
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