Method for performing multi-round fermentation by wrapping immobilized cell carbon fiber balls through porous magnetic container

Abstract

The invention discloses a method for performing multi-round fermentation by wrapping immobilized cell carbon fiber balls through a porous magnetic container. The method comprises the steps that 1, acid-forming bacterium cells are immobilized to carbon fiber carriers to form the immobilized cell carbon fiber balls; 2, the immobilized cell carbon fiber balls are contained in the anti-corrosive porous magnetic container; 3, the porous magnetic container containing the immobilized cell carbon fiber balls is put into a solid or liquid state fermentation material in a pit and the like to be fermented, the magnetic container containing the immobilized cell carbon fiber balls is attracted out from the fermentation equipment such as the pit through an electromagnetic crane after fermentation is finished, and the next round of fermentation is performed after activating is performed. The immobilized cells can be repeatedly used in the fermentation process by being combined with the magnetic porous bioreactor through repeated activation and use, and then the continuous production purpose is achieved; the high mass transfer efficiency is achieved, multi-round fermentation can be performed, the operation steps are simplified, labor is saved, and the production efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of brewing, and in particular relates to a method for wrapping immobilized cell carbon fiber balls in a porous magnetic container for multiple rounds of fermentation. Background technique [0002] Immobilized cells are a new technology developed on the basis of immobilized enzymes, that is, a basic technology that uses chemical or physical means to locate free cells in a limited space and keep them active and reusable. Immobilized cells maintain the vitality of cells. It is not only superior to the fermentation of free cells, but also has more advantages than immobilized enzymes, such as: it eliminates the procedure of breaking cells to extract intracellular enzymes, reduces Preparation cost; short cell growth stagnation time, many cells, fast response, strong anti-pollution ability, continuous fermentation, repeated use, low application cost; high stability of the enzyme in the intracellular environment, af...

AI Technical Summary

Problems solved by technology

In the existing immobilized cell fermentation, a single strain of pure strains or a mixed fermentation of limited strains are generally used. The bioreactor used is a closed container solid-state fermentation reactor with a complex structure. The disadvantage is that the mass transfer during the fermentation process The efficiency is low, the reactor needs to be manually transported after the fermentation, the equipment investment is large, the operation energy consumption is high, and it is difficult to realize continuous production, especially for multiple rounds of fermentation, the operation intensity and difficulty are relatively high Meet the needs of multiple rounds of fermentation

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