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Preparation method of high-content tremella polysaccharide

A tremella polysaccharide, high-content technology, applied in the field of preparation of high-content Tremella polysaccharide, can solve the problems of high cost of tremella polysaccharide preparation method, unsuitable for large-scale industrial production, unenvironmental protection of raw materials, etc., to achieve easy promotion and saving The effect of cost and strong practicality

Active Publication Date: 2016-07-13
JIANGSU NAIQUE BIOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Purpose of the invention: In order to overcome the problems in the prior art that the preparation method of tremella polysaccharides is high in cost, complex in process, unenvironmentally friendly and unsuitable for large-scale industrial production, the present invention proposes a simple process, environmental protection and low cost , can be applied to production scale-up, easy to popularize the preparation method of high-content tremella polysaccharide with strong practicability

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A preparation method of high-content white fungus polysaccharide, comprising the steps of:

[0027] (1) Take 1kg of commercially available white fungus and put it into the extraction kettle, add 100L of purified water, heat and reflux for 2 extractions, each time of heating and reflux extraction is 2h, the two extracts are filtered, mixed evenly and combined, and set aside ;

[0028] (2) Take the mixture of the extract obtained in step (1) and put it on No. 1 macroporous resin column LX-60. After the upper column is completed, use 4L of purified water to elute until the polysaccharide is not detected in the effluent, and stop the elution. Collect the eluate for later use; the detection of polysaccharides uses α-naphthol reaction;

[0029] (3) Take the eluate obtained in step (2) and put it on the No. 2 macroporous resin column LX-10. After the column is loaded, use 4L of purified water to elute until the effluent cannot detect polysaccharides, stop the elution, and col...

Embodiment 2

[0033] A preparation method of high-content white fungus polysaccharide, comprising the steps of:

[0034] (1) Take 1kg of commercially available white fungus and put it into the extraction kettle, add 100L of purified water, heat and reflux for 2 extractions, each time of heating and reflux extraction is 2h, the two extracts are filtered, mixed evenly and combined, and set aside ;

[0035] (2) Take the mixture of the extract obtained in step (1) and put it on No. 1 macroporous resin column LX-60. After the upper column is completed, use 4L of purified water to elute until the polysaccharide is not detected in the effluent, and stop the elution. Collect the eluate for later use; the detection of polysaccharides uses α-naphthol reaction;

[0036] (3) Take the eluate obtained in step (2) and put it on the No. 2 macroporous resin column LX-10. After the column is loaded, use 4L of purified water to elute until the effluent cannot detect polysaccharides, stop the elution, and col...

Embodiment 3

[0040] A preparation method of high-content white fungus polysaccharide, comprising the steps of:

[0041] (1) Take 0.5 kg of commercially available white fungus and put it into an extraction kettle, add 100 L of purified water, heat and reflux for 4 extractions, each heating and reflux extraction takes 3 hours, and the two extracts are filtered and mixed evenly and combined, spare;

[0042] (2) Take the mixture of the extract obtained in step (1) and put it on No. 1 macroporous resin column HPD-500. After the upper column is completed, use 3L of purified water to elute until the effluent cannot detect polysaccharides, and stop the elution. Collect the eluate for later use; the detection of polysaccharides uses α-naphthol reaction;

[0043] (3) Take the eluate obtained in step (2) and put it on the No. 2 macroporous resin column HPD-100. After the column is completed, use 3L of purified water to elute until the effluent cannot detect polysaccharides, stop the elution, and col...

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Abstract

The invention discloses a preparation method of a high-content tremella polysaccharide.The preparation method comprises the steps that firstly, heating reflux is adopted for extraction, and then two-time macroporous resin purification is performed; secondly, deconperssion concentration, 95% ethanol precipitation, standing, filtering and decompression drying are sequentially performed; finally, a tremella polysaccharide extract is obtained through a smashing process, the pigment, protein and other impurities of the tremella polysaccharide are effectively removed by adopting a method of using two different types of macroporous adsorption resin in combination, and accordingly the high-content tremella polysaccharide is obtained.In addition, a macroporous adsorption resin column can be repeatedly used, resources are reasonably utilized, and the costs are also saved.Therefore, the method is easy to popularize, has very strong practicability and is applicable to production and amplification, and used materials and by-products are non-toxic, environmentally friendly and worthy of popularization.

Description

technical field [0001] The invention relates to the field of extraction and preparation of plants, in particular to a method for preparing tremella polysaccharide with high content. Background technique [0002] Tremella is the fruiting body of Tremellaceae Tremella, also known as white fungus, white fungus, etc. It belongs to the fungus Tremellaceae Tremella, has the laudatory title of "the crown of fungi", and is more commonly planted in my country. Tremella polysaccharide is an active polysaccharide extracted from Tremella fruiting bodies or spores. A large number of studies at home and abroad have proved that tremella polysaccharides have excellent physiological effects such as improving human immune function, anti-virus, anti-tumor, anti-ulcer, lowering blood fat, lowering blood sugar, and lowering cholesterol, and tremella polysaccharides have extremely strong moisturizing properties and thickening stability. Its application as a natural moisturizer has been widely v...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003C08B37/006
Inventor 金显友易铭钟慧魏元刚杨永安
Owner JIANGSU NAIQUE BIOLOGICAL ENG
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