Preparation method of high-content tremella polysaccharide
A tremella polysaccharide, high-content technology, applied in the field of preparation of high-content Tremella polysaccharide, can solve the problems of high cost of tremella polysaccharide preparation method, unsuitable for large-scale industrial production, unenvironmental protection of raw materials, etc., to achieve easy promotion and saving The effect of cost and strong practicality
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Embodiment 1
[0026] A preparation method of high-content white fungus polysaccharide, comprising the steps of:
[0027] (1) Take 1kg of commercially available white fungus and put it into the extraction kettle, add 100L of purified water, heat and reflux for 2 extractions, each time of heating and reflux extraction is 2h, the two extracts are filtered, mixed evenly and combined, and set aside ;
[0028] (2) Take the mixture of the extract obtained in step (1) and put it on No. 1 macroporous resin column LX-60. After the upper column is completed, use 4L of purified water to elute until the polysaccharide is not detected in the effluent, and stop the elution. Collect the eluate for later use; the detection of polysaccharides uses α-naphthol reaction;
[0029] (3) Take the eluate obtained in step (2) and put it on the No. 2 macroporous resin column LX-10. After the column is loaded, use 4L of purified water to elute until the effluent cannot detect polysaccharides, stop the elution, and col...
Embodiment 2
[0033] A preparation method of high-content white fungus polysaccharide, comprising the steps of:
[0034] (1) Take 1kg of commercially available white fungus and put it into the extraction kettle, add 100L of purified water, heat and reflux for 2 extractions, each time of heating and reflux extraction is 2h, the two extracts are filtered, mixed evenly and combined, and set aside ;
[0035] (2) Take the mixture of the extract obtained in step (1) and put it on No. 1 macroporous resin column LX-60. After the upper column is completed, use 4L of purified water to elute until the polysaccharide is not detected in the effluent, and stop the elution. Collect the eluate for later use; the detection of polysaccharides uses α-naphthol reaction;
[0036] (3) Take the eluate obtained in step (2) and put it on the No. 2 macroporous resin column LX-10. After the column is loaded, use 4L of purified water to elute until the effluent cannot detect polysaccharides, stop the elution, and col...
Embodiment 3
[0040] A preparation method of high-content white fungus polysaccharide, comprising the steps of:
[0041] (1) Take 0.5 kg of commercially available white fungus and put it into an extraction kettle, add 100 L of purified water, heat and reflux for 4 extractions, each heating and reflux extraction takes 3 hours, and the two extracts are filtered and mixed evenly and combined, spare;
[0042] (2) Take the mixture of the extract obtained in step (1) and put it on No. 1 macroporous resin column HPD-500. After the upper column is completed, use 3L of purified water to elute until the effluent cannot detect polysaccharides, and stop the elution. Collect the eluate for later use; the detection of polysaccharides uses α-naphthol reaction;
[0043] (3) Take the eluate obtained in step (2) and put it on the No. 2 macroporous resin column HPD-100. After the column is completed, use 3L of purified water to elute until the effluent cannot detect polysaccharides, stop the elution, and col...
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