Preparation method of high-content tremella polysaccharide

A tremella polysaccharide, high-content technology, applied in the field of preparation of high-content Tremella polysaccharide, can solve the problems of high cost of tremella polysaccharide preparation method, unsuitable for large-scale industrial production, unenvironmental protection of raw materials, etc., to achieve easy promotion and saving The effect of cost and strong practicality

Active Publication Date: 2016-07-13

JIANGSU NAIQUE BIOLOGICAL ENG

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AI-Extracted Technical Summary

Problems solved by technology

[0006] Purpose of the invention: In order to overcome the problems in the prior art that the preparation method of tremella polysaccharides is high in cost, complex in process, unenvironmentally friendly and unsuitable for large-scale industrial p...

Abstract

The invention discloses a preparation method of a high-content tremella polysaccharide.The preparation method comprises the steps that firstly, heating reflux is adopted for extraction, and then two-time macroporous resin purification is performed; secondly, deconperssion concentration, 95% ethanol precipitation, standing, filtering and decompression drying are sequentially performed; finally, a tremella polysaccharide extract is obtained through a smashing process, the pigment, protein and other impurities of the tremella polysaccharide are effectively removed by adopting a method of using two different types of macroporous adsorption resin in combination, and accordingly the high-content tremella polysaccharide is obtained.In addition, a macroporous adsorption resin column can be repeatedly used, resources are reasonably utilized, and the costs are also saved.Therefore, the method is easy to popularize, has very strong practicability and is applicable to production and amplification, and used materials and by-products are non-toxic, environmentally friendly and worthy of popularization.

Technology Topic

ChemistryEnvironmentally friendly +7

Examples

Experimental program(3)

Example Embodiment

[0025] Example 1:

[0026] A method for preparing high-content Tremella polysaccharides includes the following steps:

[0027] (1) Take 1kg of commercially available Tremella and put it into the extraction kettle, add 100L of purified water, heat and reflux for two extractions, each time of heating and reflux extraction is 2h, the two extracts are filtered and then mixed evenly and combined for use. ;

[0028] (2) Take the mixture of the extract obtained in step (1) on No. 1 macroporous resin column LX-60. After the column is applied, it is eluted with 4L of purified water until no polysaccharide can be detected in the effluent, and the elution is stopped. Collect the eluate for later use; the detection of polysaccharides adopts α-naphthol reaction;

[0029] (3) Take the eluate obtained in step (2) on No. 2 macroporous resin column LX-10. After the column is loaded, use 4L of purified water to elute until no polysaccharide is detected in the effluent, stop elution, and collect The eluent is reserved for use; the detection of polysaccharides adopts α-naphthol reaction;

[0030] (4) Take the eluate of step (3) and concentrate it to 4L under reduced pressure at 70℃, slowly add 12L of 95% ethanol, let stand for 12h, filter, and dry under reduced pressure at 70℃. After being pulverized to 80 mesh, 203.5 g of Tremella polysaccharides with a UV content of 97.8% were obtained. The polysaccharide content was determined by the sulfuric acid-phenol method.

[0031] Wherein, the order of steps (2) and (3) can be reversed.

Example Embodiment

[0032] Example 2:

[0033] A method for preparing high-content Tremella polysaccharides includes the following steps:

[0034] (1) Take 1kg of commercially available Tremella and put it into the extraction kettle, add 100L of purified water, heat and reflux for two extractions, each time of heating and reflux extraction is 2h, the two extracts are filtered and then mixed evenly and combined for use. ;

[0035] (2) Take the mixture of the extract obtained in step (1) on No. 1 macroporous resin column LX-60. After the column is applied, it is eluted with 4L of purified water until no polysaccharide can be detected in the effluent, and the elution is stopped. Collect the eluate for later use; the detection of polysaccharides adopts α-naphthol reaction;

[0036] (3) Take the eluate obtained in step (2) on No. 2 macroporous resin column LX-10. After the column is loaded, use 4L of purified water to elute until no polysaccharide is detected in the effluent, stop elution, and collect The eluent is reserved for use; the detection of polysaccharides adopts α-naphthol reaction;

[0037] (4) Take the eluent of step (3) and concentrate it to 8L under reduced pressure at 70℃, slowly add 24L of 95% ethanol, let stand for 24h, filter, and dry under reduced pressure at 70℃. After being pulverized to 60 mesh, 387.5 g of Tremella polysaccharide with a UV content of 92.1% was obtained; the polysaccharide content was determined by the sulfuric acid-phenol method.

[0038] Wherein, the order of steps (2) and (3) can be reversed.

Example Embodiment

[0039] Example 3:

[0040] A method for preparing high-content Tremella polysaccharides includes the following steps:

[0041] (1) Take 0.5 kg of commercially available Tremella and put it into the extraction kettle, add 100L of purified water, heat and reflux for 4 times of extraction, each time of heating and reflux extraction is 3h, the two extracts are filtered and mixed evenly and combined. spare;

[0042] (2) Take the mixture of the extract obtained in step (1) on No. 1 macroporous resin column HPD-500. After the column is applied, it is eluted with 3L of purified water until no polysaccharide can be detected in the effluent, and the elution is stopped. Collect the eluate for later use; the detection of polysaccharides adopts α-naphthol reaction;

[0043] (3) Take the eluate obtained in step (2) on the No. 2 macroporous resin column HPD-100. After the column is loaded, use 3L of purified water to elute until no polysaccharide can be detected in the effluent, stop the elution, and collect The eluent is reserved for use; the detection of polysaccharides adopts α-naphthol reaction;

[0044] (4) Take the eluate of step (3) and concentrate it to 3L under reduced pressure at 70℃, slowly add 60L of 95% ethanol, let stand for 6h, filter, and dry under reduced pressure at 70℃. After being pulverized to 100 mesh, 214.6g of Tremella polysaccharide with a UV content of 91.1% was obtained; the polysaccharide content was determined by the sulfuric acid-phenol method.

[0045] Wherein, the order of steps (2) and (3) can be reversed.

[0046] From the data in Examples 1-3, it can be seen that the method for preparing high-content Tremella polysaccharides provided by the present invention effectively removes the pigment, protein, and protein in Tremella polysaccharides through the combined use of two different macroporous adsorption resins. Other impurities, so as to obtain high content of Tremella fuciformis polysaccharide; and the macroporous adsorption resin column can be used repeatedly, which not only rationally utilizes resources but also saves costs, so this method is easy to popularize, has strong practicability, is suitable for production scale-up, and The raw materials and by-products used are environmentally friendly and non-toxic, and are worthy of promotion.

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Classification and recommendation of technical efficacy words

rational use of resources
low cost

Preparation method of high-content tremella polysaccharide

AI-Extracted Technical Summary

Problems solved by technology

Abstract

Examples

Example Embodiment

Example Embodiment

Example Embodiment

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Description & Claims & Application Information

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