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A protease-producing bacterium for hydrolyzing pig blood—Bacillus amyloliquefaciens and its screening and application methods

A technology of amylolytic spores and porcine blood protein, which is applied in the field of microorganisms or enzymes, and can solve problems that have not yet been solved

Inactive Publication Date: 2019-08-23
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is no application for protease-producing bacteria that hydrolyze pig blood

Method used

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  • A protease-producing bacterium for hydrolyzing pig blood—Bacillus amyloliquefaciens and its screening and application methods
  • A protease-producing bacterium for hydrolyzing pig blood—Bacillus amyloliquefaciens and its screening and application methods
  • A protease-producing bacterium for hydrolyzing pig blood—Bacillus amyloliquefaciens and its screening and application methods

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1: Screening of protease-producing bacteria for hydrolyzing pig blood

[0051] Sampling 80g of traditional bacon, tempeh, sour meat, fermented meat, soy products, distiller's yeast, and fermented grains from Huaxi, Guiyang, China, and sealing it with polyethylene bags; sampling 80g of surface soil and silt polluted by blood in Qiandongnan Prefecture, Guizhou Province, Seal with a polyethylene bag.

[0052] Weigh 5.0g sample, add it to 50mL sterile normal saline, then add 4 small glass beads, shake at 30°C and 180r / min for 30min, take 5mL and add it to the enrichment medium, shake at 30°C and 180r / min Bed enrichment culture for 24h. The enrichment medium components used (g / 100mL) are: beef extract 0.5-1.0, peptone 0.5-1.0, (NH 4 ) 2 SO 4 0.1~0.2, K 2 HPO 4 0.5~1.0, MgSO 4 ·7H 2 O 0.01~0.02, CaCl 2 0.01-0.02, NaCl 0.5-1.0, pH 6.5-7.5.

[0053] Preliminary screening: take 0.2mL of the above-mentioned enriched bacterial solution diluted in an appropriat...

Embodiment 2

[0058] Example 2: Use of extracellular protease-producing bacteria for hydrolyzing pig blood

[0059] Take 2 loops of the slant-preserved strain Bacillus amyloliquefaciens GUHP86 and inoculate them respectively in the seed culture medium, culture them on a shaker at 30°C and 180r / min for 24 hours, and add 6% to 8% (0.1 to 5×10 6 cfu / mL) inoculated into the fermentation medium, cultured on a shaking table under the same conditions for 24 hours, and then directly used for the hydrolysis of protein in pig blood, and the degree of hydrolysis reached 18.93%.

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Abstract

A protease-producing bacterium for hydrolyzing pig blood—Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) and methods for screening and using the same. Bacillus amyloliquefaciens ), the pure culture of this strain was deposited in Wuhan China Center for Type Culture Collection on January 4, 2016, with the preservation number CCTCC NO.M 2016003; referred to as Bacillus amyloliquefaciens GUHP86. The bacillus amyloliquefaciens GUHP86 of the present invention has the property of producing protease, can be directly used for hydrolyzing porcine blood protein, and conforms to the development trend of using protease to hydrolyze protein. The advantages are low cost, recyclable utilization, good stability, green and safe, can be directly used in biocatalysis, wide source of raw materials, simple and practical method. Therefore, GUHP86 has an important application prospect in the hydrolysis of porcine blood protein, and can also be applied to the hydrolysis of other proteins.

Description

technical field [0001] The present invention relates to microorganisms or enzymes, in particular to protease-producing bacteria. Background technique [0002] Microbial proteases are mainly derived from the metabolites of microorganisms, such as some bacteria, molds, etc. For example, some Bacillus subtilis, Bacillus amyloliquefaciens, Aspergillus niger, Aspergillus flavus, Aspergillus oryzae, etc. can produce protease. [0003] Livestock blood is a rich source of protein, among which the protein content in the blood of pigs, cattle and other livestock is about 18% to 22%, containing a variety of amino acids and trace elements. Pig blood is especially a precious resource in livestock blood. The protein content in pig blood accounts for 18.9% of the total blood, so pig blood is called "liquid meat". Pig blood resources are very rich in my country, but because of its high water content, pig blood is easily polluted and corrupted. In addition, pig blood has poor palatability ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P21/00C12R1/07
CPCC12N1/20C12P21/00C12N1/205C12R2001/07
Inventor 何腊平张明赞李翠芹张义明
Owner GUIZHOU UNIV