Biomarker-spectrum-based lung cancer risk prediction kit for high-risk groups in rural China

A technology of biomarkers and kits, applied in the field of medical biology, can solve problems such as unreported, loss of radical surgery, difficult to achieve, etc., and achieve the effect of simple expression level

Active Publication Date: 2016-07-13
上海超联智医学科技中心(有限合伙)
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  • Abstract
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  • Application Information

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Problems solved by technology

However, among the vast rural population in my country, it is difficult to carry out large-scale CT screening in a short period of time. As a result, when lung cancer is diagnosed, it is already in the middle and advanced stages of lung cancer, and the chanc

Method used

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  • Biomarker-spectrum-based lung cancer risk prediction kit for high-risk groups in rural China

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Experimental program
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Effect test

Embodiment 1

[0031] The composition of the kit of the invention:

[0032] Consists of biomarkers and enzyme-labeled antibodies, carbonate buffer with pH 9.6, phosphate buffer with pH 7.4, serum protein diluent, stop solution, tetramethylbenzidine substrate solution, normal Composition of serum and positive control serum.

[0033] The biomarkers include gastrin release propeptide, carcinoembryonic antigen, fragments of cytokeratin 19 and squamous cell carcinoma antigen; the carbonate buffer with a pH of 9.6 is composed of 1.59 grams of sodium carbonate and 2.93 grams of sodium bicarbonate is added to 1L of distilled water; the phosphate buffer with a pH of 7.4 is 0.2 grams of potassium dihydrogen phosphate, 2.9 grams of disodium hydrogen phosphate dodecahydrate, 8.0 grams of sodium chloride, 0.2 Grams of potassium chloride, 0.5mL Tween-20 added to 1L of distilled water; the serum protein diluent is 0.1 grams of bovine serum, goat serum or rabbit serum protein added to 100mL of phosphate buffer ...

Embodiment 2

[0048] The present invention collects 715 patients from the rural population who have been clinically determined to have high risk factors for lung cancer, and finally diagnoses 434 lung cancer patients by pathology. The serum of 154 lung cancer patients is sampled by randomization method; at the same time, in the serum bank of 281 non-lung cancer patients, 235 normal human sera were sampled by randomization method. Chemiluminescence microparticle immunoassay was used to detect four biomarkers in serum, and clinical information such as patient age, smoking history, gender and so on was collected. The obtained model sensitivity and specificity are shown in Table 1. The ROC curve of a lung cancer risk prediction model based on biomarker profiles for high-risk groups in rural China is as follows figure 1 Shown.

[0049] Table 1 Effectiveness of lung cancer risk prediction model based on biomarker profile for high-risk groups in rural China

[0050]

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Abstract

The invention relates to a biomarker-spectrum-based lung cancer risk prediction kit for high-risk groups in rural China.The kit comprises reagents for measuring the serum protein spectrum expression quantity of the four biomarkers comprising gastrin release propeptide, the carcino-embryonic antigen, the cytokeratin 19 fragment and the squamous-cell carcinoma antigen.The kit is combined with other clinical indexes such as the age, smoking history, malignancy history and sex, and can be used for accurately predicting the lung cancer risk.

Description

Technical field [0001] The invention relates to the field of medical biotechnology, in particular to a lung cancer risk prediction kit for high-risk groups of rural populations in China based on a biomarker profile. Background technique [0002] Lung cancer is the most common cancer worldwide and has the highest fatality rate. In my country, the incidence and mortality of lung cancer are the highest among all malignant tumors. According to the statistics of The International Agency for Research on Cancer (IARC) of the World Health Organization, the incidence of lung cancer in my country in 2008 was 35 / 100,000, and the agency estimates that by 2025, my country will add 1 million new cases of lung cancer each year. According to the 2010 Health Statistics Yearbook of the Ministry of Health of my country, as of 2009, the national lung cancer death rate was 30.83 per 100,000 people, which is more than 4 per 100,000 people with liver cancer, the second highest death rate. Although th...

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Application Information

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IPC IPC(8): G01N33/74G01N33/574
CPCG01N33/57423G01N33/57473G01N33/57484G01N33/74G01N2333/47G01N2333/5758
Inventor 白春学杨达伟周建
Owner 上海超联智医学科技中心(有限合伙)
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