A lung cancer risk prediction kit for high-risk groups in rural China based on biomarker profiles

A high-risk, rural technology, applied in the field of medical biology, can solve problems such as unreported, difficult to achieve, loss of radical surgery, etc., and achieve the effect of simple expression level

Active Publication Date: 2018-03-27
上海超联智医学科技中心(有限合伙)
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Problems solved by technology

However, among the vast rural population in my country, it is difficult to carry out large-scale CT screening in a short period of time. As a result, when lung cancer is diagnosed, it is already in the middle and advanced stages of lung cancer, and the chance of radical surgery has been lost.
Therefore, there is an urgent need for a lung cancer risk prediction kit that does not rely on CT imaging technology and is suitable for high-risk groups in rural China, but there is no report on this kind of kit at present

Method used

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  • A lung cancer risk prediction kit for high-risk groups in rural China based on biomarker profiles
  • A lung cancer risk prediction kit for high-risk groups in rural China based on biomarker profiles

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Experimental program
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Embodiment 1

[0031] The composition of kit of the present invention:

[0032] Composed of biomarkers and their enzyme-labeled antibodies, carbonate buffer solution with a pH value of 9.6, phosphate buffer solution with a pH value of 7.4, serum protein dilution, stop solution, tetramethylbenzidine substrate solution, and normal people Serum and positive control serum composition.

[0033] The biomarkers include gastrin-releasing propeptide, carcinoembryonic antigen, cytokeratin 19 fragments and squamous cell carcinoma antigen; Add 2.93 grams of sodium bicarbonate to 1L of distilled water and obtain; the phosphate buffer solution with a pH value of 7.4 is 0.2 grams of potassium dihydrogen phosphate, 2.9 grams of disodium hydrogen phosphate dodecahydrate, 8.0 grams of sodium chloride, 0.2 gram potassium chloride, 0.5mL Tween-20 was added to 1L distilled water and obtained; the serum protein dilution was 0.1 gram bovine serum, goat serum or rabbit serum protein added to 100mL phosphate buffer...

Embodiment 2

[0048]The present invention collects 715 rural population patients with high risk factors of lung cancer determined clinically, and finally diagnoses 434 cases of lung cancer through pathology, and samples the serum of 154 lung cancer patients through a randomized method; at the same time, in the serum bank of 281 non-lung cancer patients, Sera from 235 normal persons were sampled by random method. Chemiluminescent microparticle immunoassay was used to detect 4 biomarkers in serum, and clinical information such as age, smoking history, and gender of patients were collected at the same time. The sensitivity and specificity of the obtained models are shown in Table 1. The ROC curve of the lung cancer risk prediction model for high-risk groups in rural China based on biomarker profiles is as follows: figure 1 shown.

[0049] Table 1 The validity of the lung cancer risk prediction model based on biomarker profiles for high-risk groups in rural China

[0050]

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Abstract

The invention relates to a biomarker-spectrum-based lung cancer risk prediction kit for high-risk groups in rural China.The kit comprises reagents for measuring the serum protein spectrum expression quantity of the four biomarkers comprising gastrin release propeptide, the carcino-embryonic antigen, the cytokeratin 19 fragment and the squamous-cell carcinoma antigen.The kit is combined with other clinical indexes such as the age, smoking history, malignancy history and sex, and can be used for accurately predicting the lung cancer risk.

Description

technical field [0001] The invention relates to the field of medical biotechnology, in particular to a lung cancer risk prediction kit for Chinese rural high-risk groups based on biomarker spectrum. Background technique [0002] Lung cancer is the most common and deadly cancer worldwide. In my country, the morbidity and mortality of lung cancer rank first among all malignant tumors. According to the statistics of The International Agency for Research on Cancer (IARC) of the World Health Organization, the incidence of lung cancer in my country in 2008 was 35 per 100,000 people, and the agency estimated that by 2025, there will be 1 million new cases of lung cancer in my country every year . According to the 2010 Health Statistical Yearbook of the Ministry of Health of my country, as of 2009, the national lung cancer mortality rate was 30.83 / 100,000 people, which was higher than the second highest mortality rate of liver cancer by more than 4 / 100,000 people. Although the 5-y...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/74G01N33/574
CPCG01N33/57423G01N33/57473G01N33/57484G01N33/74G01N2333/47G01N2333/5758
Inventor 白春学杨达伟周建
Owner 上海超联智医学科技中心(有限合伙)
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