Tissue culture method for abelmoschus esculentus

Abstract

The invention discloses a tissue culture method for abelmoschus esculentus and belongs to the technical field of plant biology. The method comprises the following steps of: preparing an explant, selecting mature and full abelmoschus esculentus seeds, flushing the abelmoschus esculentus seeds with running water for 2-3 hours, then disinfecting the abelmoschus esculentus seeds with 70 percent of alcohol in a clean bench for 30s, disinfecting with 0.1 percent of mercuric chloride for 8-10 minutes, flushing with aquae sterilisata for 5-6 times, and soaking the disinfected seeds in the aquae sterilisata for 12-24 hours; inoculating the soaked seeds into a 1 / 2 MS culture medium, and culturing under lights of a fluorescent lamp for 5-7 days; after leaves are flattened, cutting 1.5-25cm terminal buds with partial hypocotyls, inoculating the terminal buds into an MS culture medium containing 0.05mg / L NAA, and culturing under lights of an LED (Light-Emitting Diode) at a blue-red-yellow combination ratio of 1 to 3 to 1 for 35 days, wherein the lights of the LED at a blue-red-yellow combination ratio of 1 to 3 to 1 is suitable for growth of abelmoschus esculentus tissue culture seedlings. The tissue culture method has the advantages of simplicity in operation, rapid plant growth, strong plants, energy conservation, environmental friendliness and the like, plays an important role in research on in-vitro rapid propagation or molecular breeding of the abelmoschus esculentus, and is high in practical application value.

Description

1. Technical field [0001] The invention relates to a tissue culture method of okra, which belongs to the field of plant biotechnology. 2. Background technology [0002] Okra (Abelmoschusesculentus L.), also known as okra, coffee umber, hollyhock, okra, hair eggplant, also known as "pepper" in the folk, is an annual herb of the Malvaceae okra, mainly feeding on tender fruits. The flowers and seeds of okra can be used as medicine, and it is rich in trace elements such as zinc and selenium, vitamin C and soluble fiber, which can enhance the body's anti-cancer and anti-cancer ability, has a health care effect on the skin, and has extremely high edible and medicinal properties. equivalent value. At present, the variety selection and breeding of okra mainly adopts the methods of artificial seed production and introduction and selection. In recent years, in order to meet the needs of cultivating hybrid seedlings, the use of in vitro rapid propagation technology to preserve okra h...

AI Technical Summary

Problems solved by technology

However, the spectral energy distribution of these light sources is designed and produced based on the principle of high-efficiency demand for light by the human eye, which contains many unnecessary wavelengths and the light efficiency is very low.

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