Detection kit for epidemic hemorrhagic fever virus

Abstract

The invention discloses a detection kit for epidemic hemorrhagic fever virus. The detection kit comprises a nanogold marked mouse-anti-human IgA antibody detection probe, an epidemic hemorrhagic fever virus mixing specific antigen and a spot gold immune concentrated infiltration device, wherein the concentrated infiltration device is composed of three parts, namely, a small plastic box, a nitrocellulose membrane added with the epidemic hemorrhagic fever virus mixing specific antigen and a water-absorbing pad. A corresponding square groove corresponding to a square hole in a box cover is formed at the center of the bottom of the small plastic box. The detection kit provided by the invention is used for detecting if the epidemic hemorrhagic fever virus antibody exists in human serum according to a spot gold immune concentrated infiltration method; if a human body is infected by the epidemic hemorrhagic fever virus can be quickly and directly judged; the detection kit provided by the invention is simply prepared; the detection is convenient and quick; the sensitivity is high; a new method for clinical diagnosis for epidemic hemorrhagic fever is supplied; the detection kit is fit for clinic application.

Description

technical field [0001] The invention relates to the technical field of disease detection, in particular to a detection kit for epidemic hemorrhagic fever virus. Background technique [0002] Hemorrhagic fever, also known as hemorrhagic fever with renal syndrome, is an important infectious disease that endangers human health. It is caused by epidemic hemorrhagic fever virus (Hantavirus) and is a natural source of epidemics with rodents as the main source of infection. disease. The main clinical manifestations are fever, hemorrhage, hyperemia, hypotensive shock and kidney damage. [0003] After the virus invades the human body, it directly acts on the capillaries and small blood vessels of the whole body, causing extensive damage to the blood vessel wall, increasing the permeability of the blood vessel wall, causing tissue or organ edema, and resulting in hyperemia or bleeding of the skin and mucous membranes of the whole body, such as the patient's Redness or bleeding point...

AI Technical Summary

Problems solved by technology

[0004] At present, the traditional detection methods for epidemic hemorrhagic fever virus antibodies are serological diagnostic methods, including indirect enzyme immunosorbent assay for detection of EHFIgG antibody, immunofluorescence test for detection of serum IgG antibody, etc. These methods are prone to false positives or false positives to a certain extent. Negative phenomenon, which brings some interference to the clinical diagnosis of epidemic hemorrhagic fever