A test strip for detecting quinclorac and its preparation method and application
A quinclorac and test strip technology, which is applied to measurement devices, instruments, scientific instruments, etc., can solve the problems that it is difficult to meet the rapid detection of a large number of samples and on-site samples, the processing process is complicated, and the cost is high, and the detection time is short, Simple operation and low cost effect
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Embodiment 1
[0041] Example 1 Preparation of test strips for detecting quinclorac
[0042] The preparation method of this test strip mainly comprises the following steps:
[0043] 1) Prepare a conjugate release pad sprayed with quinclorac monoclonal antibody-colloidal gold marker;
[0044] 2) Prepare a reaction membrane with a detection line coated with quinclorac hapten-carrier protein conjugate and a quality control line coated with goat anti-mouse anti-antibody;
[0045] 3) Assemble the conjugate release pad prepared in 1) and 2), the reaction membrane, the sample absorption pad, the water absorption pad and the PVC bottom plate into a test strip.
[0046] The following step-by-step detailed description:
[0047] 1. Synthesis of quinclorac hapten (see attached image 3 ) and identification
[0048]Take 0.43 g bromoacetyl chloride, add 10 mL chloroform to dissolve, add 30.28 g AlCl 3 Stir, react at room temperature for 2 h, add dropwise 20 mL of a pyridine solution containing 1.0 g ...
Embodiment 2
[0089] The detection of quinclorac in the sample of embodiment 2
[0090] 1. Sample pretreatment
[0091] Weigh 1.0±0.02 g soil sample into a 10 mL polystyrene centrifuge tube; add 4 mL sample extract solution (50% methanol solution), shake for 5 min; stand at room temperature (20-25 ℃) for 5 min; pipette 1 Mix mL of supernatant and 9 mL of sample diluent (0.02 mol / L PBS) into a 10 mL polystyrene centrifuge tube to obtain the sample solution to be tested.
[0092] 2. Test with test strips
[0093] Use a pipette to take 100 μl of the sample solution to be tested (2-3 drops from the dropper) and drop it vertically into the sample hole; start timing when the liquid flows, react for 10 minutes, and interpret the results.
[0094] 3. Analyze test results
[0095] Negative (-): The color of the T line is darker than that of the C line or consistent with the color of the C line, indicating that the concentration of quinclorac in the sample is lower than the detection limit, such as ...
Embodiment 3
[0098] Embodiment 3 sample detection example
[0099] 1. Detection limit test
[0100] Take blank soil samples, add quinclorac to the final concentrations of 0.4, 0.8, and 1.6 mg / kg respectively, take test strips for detection, and repeat the measurement three times for each sample.
[0101] When testing the soil sample with a test strip, when the added concentration of quinclorac is 0.4 mg / kg, the test strip shows that the color of the T line is darker than that of the C line or is consistent with the color of the C line, which is negative ; When the added concentration of quinclorac is 0.8, 1.6 mg / kg, the test strip shows that the T line color is lighter than the C line color or the T line does not develop color, which is positive, indicating that the test strip is right The detection limit of quinclorac in soil was 0.8 mg / kg.
[0102] 2. False positive rate and false negative rate test
[0103] Take 20 soil positive samples with known quinclorac content greater than 0.8 ...
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