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Method for extracting Gynura divaricata extract product and purpose thereof

A kind of extraction method, the technique of extract, applied in the field of traditional Chinese medicine

Inactive Publication Date: 2016-08-03
TIANJIN INT JOINT ACADEMY OF BIOTECH & MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, no one has used a suitable extraction process to extract Mingyuecao and confirmed that it has a hypoglycemic effect

Method used

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  • Method for extracting Gynura divaricata extract product and purpose thereof
  • Method for extracting Gynura divaricata extract product and purpose thereof
  • Method for extracting Gynura divaricata extract product and purpose thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The preparation of embodiment one Mingyuecao extract

[0026] 1. Preparation of the water extract of Mingyuecao

[0027] Weigh 1.041kg of fresh leaves of Mingyuecao, put them in a 10L round bottom flask after crushing, add 8 times the amount of pure water, heat and reflux in the electric heating mantle to extract for 1.5 hours, and filter while hot; kg) Pure water was added to a 10L round bottom flask, heated to reflux in a heating mantle for 2 hours, and filtered while it was hot. The above filtrates were combined, 11.86L (90%) of the extract was taken, concentrated under reduced pressure at 50°C, and freeze-dried to a powder state to obtain 26.3g of dry powder, i.e. sample 1-6. The flow chart is shown in Figure 1(a).

[0028] 2. Enrichment of different types of components in the water extract of Mingyuecao

[0029] Take the remaining 10% of the water extract of Crassula glabra obtained in Example 1 in a 5L beaker, add 3 times the volume of absolute ethanol, stir wel...

Embodiment 2

[0033] The efficacy experiment of embodiment two Mingyuecao extract

[0034] 1. Drug screening based on insulin resistance model

[0035] HepG2 cells in the logarithmic growth phase were divided into 10 4 Each cell / well was transferred to a 96-well cell culture plate and divided into a blank control group, a model group, a TZD (pioglitazone, Pioglitazone) group, and four Mingyuecao extract groups. The model group, TZD group and Mingyuecao extract group were incubated with dexamethasone to make the concentration reach 0.5μM; 24h later, insulin (10nmol / L) was added to each group, and the low-sugar and phenol red-free DMEM medium acted on the model group , pioglitazone (150μmol / L), Mingyuecao extract 1-6 (10mg / ml), 1-7 (10mg / ml), 1-8 (10mg / ml), 1-10 (10mg / ml) Act on TZD group and 4 Mingyuecao extract groups respectively; after 24h, measure the consumption of glucose in the medium. The administration concentrations indicated above are the final concentrations of the cell cultur...

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Abstract

The invention relates to a method for extracting a Gynura divaricata extract product and a purpose thereof. The method comprises the following steps: weighing the fresh leaves of Gynura divaricata, crushing the fresh leaves of the Gynura divaricata; placing the crushed Gynura divaricata in a container, adding water in the container, wherein the weight of water accounts for 6-10 times of that of the Gynura divaricata; performing heating reflux for 1-3 hours, filtering while hot to obtain a first filtrate and filter residues; adding the filter residues and 3-7 times the weight of water to the container, performing heating reflux for 1-3 hours, filtering while hot to obtain a second filtrate; merging the first filtrate and the second filtrate to obtain a third filtrate; and performing vacuum concentration on the third filtrate and performing freeze drying on the third filtrate to obtain first drying powder. The Gynura divaricata extract product has effect for lowering blood sugar, and has good inhibition and treatment effects for type 2 diabetes.

Description

technical field [0001] The present invention relates to the field of traditional Chinese medicine, and more specifically relates to the extraction method and application of the extract of Mingyuecao. Background technique [0002] Nowadays, people's quality of life is gradually improving, and dietary conditions and habits are changing, which makes the incidence of diabetes increase year by year. Hyperglycemia and some chronic complications caused by diabetes increase the suffering of patients. Therefore, reducing blood sugar in diabetic patients, relieving patients' suffering and reducing the burden on the country have always been concerned by scholars at home and abroad. Since some of the existing oral and injection hypoglycemic drugs have large side effects and are prone to dependence, we need to search for therapeutic drugs with good curative effect and low side effects in the field of traditional Chinese medicine. [0003] The inventor has conducted research on many plan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/23A61P3/10
Inventor 张博李金楠刘祥魏宏冯金磊王淑艳张晶晶杨诚
Owner TIANJIN INT JOINT ACADEMY OF BIOTECH & MEDICINE