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Detection chip based on surface enhanced raman scattering technique, preparation method and kit

A surface-enhanced Raman and detection chip technology, applied in Raman scattering, measuring devices, instruments, etc., can solve the problems of joint detection of tumor markers, etc., to improve sensitivity and accuracy, ensure accuracy, and good reproducibility sexual effect

Active Publication Date: 2016-08-03
INST OF HIGH ENERGY PHYSICS CHINESE ACADEMY OF SCI
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Problems solved by technology

[0005] In order to overcome the defect that multiple tumor markers cannot be jointly detected in the prior art, the purpose of the present invention is to provide a detection chip based on surface-enhanced Raman scattering (SERS) technology, which can detect multiple markers at the same time, and the accuracy , better sensitivity

Method used

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  • Detection chip based on surface enhanced raman scattering technique, preparation method and kit
  • Detection chip based on surface enhanced raman scattering technique, preparation method and kit
  • Detection chip based on surface enhanced raman scattering technique, preparation method and kit

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Embodiment

[0044] Such as figure 1 As shown, taking the detection of two markers as an example, the signal molecule MBA was first printed on the silver island substrate with a polydimethylsiloxane (PDMS) stamp with a microarray structure on the surface. Then grow the signal molecule DSNB in ​​other regions to form a surface functional micro-domain structure with uniform and spaced distribution of various signal molecules; control the reaction conditions to make probe 1 and probe 2 specifically bind to signal molecule 1 and signal molecule 2 successively; Finally, probe 1 and probe 2 specifically hybridize with the target analyte respectively, and affect the structure of the signal molecule at the bottom, thereby generating a peak shift. The specific process is as follows:

[0045] 1. Silver Island Substrate Preparation

[0046] 1×1cm 2Silicon / glass wafers were ultrasonicated in ethanol and acetone for 10 min respectively, dried and placed in piranha washing solution (H 2 SO 4 / H 2 ...

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Abstract

The invention provides a detection chip based on a surface enhanced raman scattering technique. The detection chip comprises a nano-metal base, two or more signal molecules and two or more probe sequences, wherein the signal molecules are uniformly distributed on the nano-metal base and the probe sequences are used for detecting tumor markers and are fixed on the different signal molecules, respectively. The invention also provides a preparation method of the detection chip and a detection kit containing the detection chip. The detection chip and the detection kit provided by the invention can realize the one-step, simultaneous, sensitive and quantitative determination on various tumor markers by combining with stress sensing technique and by fixing different raman signal molecules in different microcells. The detection chip and the detection kit have huge application potentials.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a detection chip based on surface-enhanced Raman scattering technology, a preparation method thereof and a kit containing the detection chip. Background technique [0002] Tumor markers microRNAs (miRNAs) show different expression levels along with the occurrence, growth, metastasis and treatment of tumors. Quantitative and highly sensitive detection of miRNA can be used to diagnose early tumors and improve the survival rate of patients, which is of great significance in the diagnosis and treatment of tumors. The current quantitative detection methods for miRNAs include Northernblotting, QT-PCR, fluorescence method, microarray method, SERS method and so on. [0003] At present, the dual-temperature hybridization method in the SERS (Surface Enhanced Raman Scattering) method can only detect one miRNA at a time, and there are many steps. The process is as follows: a capture pro...

Claims

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Application Information

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IPC IPC(8): G01N21/65
CPCG01N21/658G01N21/65
Inventor 李敏朱文凤赵宇亮
Owner INST OF HIGH ENERGY PHYSICS CHINESE ACADEMY OF SCI
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