Hierarchical ordered macroporous-mesoporous monolithic silica column bovine serum albumin imprinted polymer and preparation method thereof

A bovine serum albumin and serum albumin technology, applied in chemical instruments and methods, other chemical processes, etc., can solve the problems of large size of protein molecules, difficult mass transfer, lack of selectivity, etc., and achieve strong permeability and transmission. The effect of fast mass kinetics and improved selectivity

Active Publication Date: 2016-08-10
上海健士拜生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Western blotting polymers are rarely reported, especially macromolecular western blotting polymers
Protei

Method used

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  • Hierarchical ordered macroporous-mesoporous monolithic silica column bovine serum albumin imprinted polymer and preparation method thereof
  • Hierarchical ordered macroporous-mesoporous monolithic silica column bovine serum albumin imprinted polymer and preparation method thereof

Examples

Experimental program
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Example Embodiment

[0034] Example 1:

[0035] (1) 0.4 g of polystyrene was dissolved in 8 mL of water, the solution was sonicated for 20 minutes and allowed to settle naturally for a week, the upper liquid was carefully removed, and dried under vacuum at 25° C. for 48 hours to obtain polystyrene colloidal crystals.

[0036] (2) 2g TEOS, 0.9g water, 0.8g hydrochloric acid, 1.0g P123 and 15g absolute ethanol are prepared as a precursor solution. 0.4g polystyrene colloidal crystals are added to the solution and immersed for 1h, where the concentration of hydrochloric acid is 2mol / L. Then immerse and evaporate at 30°C for 48h. Place the synthesized block material in a tube furnace at 1℃·min in air -1 The temperature rise rate of the temperature rises slowly, and the temperature is kept at 550°C for 6 hours to obtain a hierarchically ordered macroporous-mesoporous silica monolith (HOM).

[0037] (3) 0.4 g of HOM obtained in step (2) is dissolved in 40 mL of absolute ethanol. Then add 2mL MPS to the mixe...

Example Embodiment

[0041] Example 2:

[0042] (1) 0.8 g of polystyrene was dissolved in 8 mL of water, the solution was sonicated for 20 minutes and naturally settled for a week, the upper liquid was carefully removed, and the polystyrene was dried under vacuum at 25° C. for 48 hours to obtain polystyrene colloidal crystals.

[0043] (2) 1.5g TEOS, 0.9g water, 0.1g hydrochloric acid, 1.0g P123 and 15g absolute ethanol are prepared as a precursor solution, 0.6g polystyrene colloidal crystals are added to the solution and immersed for 1h, where the concentration of hydrochloric acid is 2mol / L. Then immerse and evaporate at 30°C for 48h. Place the synthesized block material in a tube furnace at 1℃·min in air -1 The temperature rise rate of the temperature rises slowly, and the temperature is kept at 550°C for 6 hours to obtain a hierarchically ordered macroporous-mesoporous silica monolith (HOM).

[0044] (3) 0.4 g of HOM obtained in step (2) is dissolved in 40 mL of absolute ethanol. Then add 2mL MPS...

Example Embodiment

[0046] Example 3:

[0047] (1) 1.0 g of polystyrene was dissolved in 8 mL of water, the solution was sonicated for 20 minutes and allowed to settle naturally for one week, the upper liquid was carefully removed, and the polystyrene was dried under vacuum at 25° C. for 48 hours to obtain polystyrene colloidal crystals.

[0048] (2) 2.5g TEOS, 0.9g water, 0.4g hydrochloric acid, 1.0g P123 and 15g absolute ethanol are prepared as a precursor solution. 1.0g polystyrene colloidal crystals are added to the solution and immersed for 1h, where the concentration of hydrochloric acid is 2mol / L. Then immerse and evaporate at 30°C for 48h. Place the synthesized block material in a tube furnace at 1℃·min in air -1 The temperature rise rate of the temperature rises slowly, and the temperature is kept at 550°C for 6 hours to obtain a hierarchically ordered macroporous-mesoporous silica monolith (HOM).

[0049] (3) 0.4 g of HOM obtained in step (2) is dissolved in 40 mL of absolute ethanol. Then...

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Abstract

The invention provides a hierarchical ordered macroporous-mesoporous monolithic silica column bovine serum albumin imprinted polymer and a preparation method thereof. The preparation method comprises the following steps: synthesis of polystyrene colloidal crystal; synthesis of a hierarchical ordered macroporous-mesoporous monolithic silica column; preparation of gamma-(methyacryloyloxy)propyltrimethoxysilane modification material for the hierarchical ordered macroporous-mesoporous monolithic silica column; and preparation of the hierarchical ordered macroporous-mesoporous monolithic silica column bovine serum albumin imprinted polymer. The synthesized hierarchical ordered macroporous-mesoporous monolithic silica column has regular macroscopic and microscopic structures, good permeability, low backpressure and fast mass transfer kinetic action; the recognition site of the synthesized bovine serum albumin imprinted polymer is located on or close to the surface of the polymer, which facilitates bovine serum albumin molecules in entering and exiting from imprinting holes and improves selectivity of bovine serum albumin; and the hierarchical ordered macroporous-mesoporous monolithic silica column bovine serum albumin imprinted polymer has good bovine serum albumin separation performance and has adsorption capacity of 121.6 mg/g.

Description

technical field [0001] The invention relates to a silica gel monolithic column imprinted polymer, in particular to a hierarchically ordered porous silica gel monolithic column imprinted polymer and a preparation method thereof, belonging to the technical fields of material preparation and bioengineering. Background technique [0002] Solid-phase extraction (Solid-Phase Extraction, SPE) is a sample pretreatment technology developed in recent years, which is mainly used for the separation and purification of samples. It is easy to operate, saves time and effort, and can more effectively separate analytes from interfering components, and is widely used in medicine, food, chemical and other fields. Separation columns for solid phase extraction are usually packed with particles of controlled size. The smaller the particle diameter, the higher the separation efficiency, but the back pressure also increases. Recently, the concept of monolithic columns has been introduced into sep...

Claims

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Application Information

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IPC IPC(8): C08F222/38C08F220/56C08J9/26B01J20/26B01J20/30
CPCB01J20/268C08F222/385C08J9/26C08J2201/042C08J2335/00C08F220/56
Inventor 刘燕刘方方倪良孟敏佳钟国星
Owner 上海健士拜生物科技有限公司
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