A process for efficiently extracting γ-polyglutamic acid from fermentation broth
A technology of polyglutamic acid and fermentation broth, applied in the biological field, can solve the problems of low recovery rate of γ-PGA, consumption of organic solvents, increase of production costs, etc., to improve extraction efficiency and product purity, reduce organic solvents, and production costs Reduced effect
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Embodiment 1
[0028] A process for efficiently extracting gamma-polyglutamic acid from fermentation broth, comprising the following steps:
[0029] Step (1): Centrifuge the γ-PGA fermentation broth obtained by 100L microbial fermentation in a centrifuge at a speed of 5000rpm for 30 minutes, and separate and remove a large amount of insoluble fermentation residual nutrients and bacteria in the fermentation broth;
[0030] Step (2): adding trichloroacetic acid to the fermented liquid after centrifugation in step (1) to adjust the pH to 3, remove impurities in the fermented liquid, reduce the viscosity of the fermented liquid, and facilitate the subsequent purification process;
[0031] Step (3): Add 20g / L diatomaceous earth to absorb the residual bacteria in the fermentation broth per liter of step (2), filter or centrifuge to remove insoluble matter, then add 5g / L activated carbon to absorb the residual bacteria in the fermentation broth Pigment and other impurities are centrifuged in a cent...
Embodiment 2
[0037] A process for efficiently extracting gamma-polyglutamic acid from fermentation broth, comprising the following steps:
[0038] Step (1): Add 10 kg of solid substrate containing γ-PGA obtained by microbial solid fermentation, add 10 times the volume of distilled water, and stir and extract at 40°C for 1 hour. The extract is first filtered through a 100-mesh screen to remove residue, and the filtrate is passed through separately Filter through a 5 μm pore size filter bag and a 0.5 μm microporous filter to collect the extract.
[0039] Step (2): adding trichloroacetic acid to the extract of step (1) to adjust the pH value to 4;
[0040] Step (3): Add 10g / L diatomaceous earth and 8g / L activated carbon to absorb the pigment and other impurities in the fermentation broth in the extract of step (2), centrifuge in a centrifuge with a speed of 10000rpm for 25min, separate and obtain the above Serum;
[0041] Step (4): taking a sample from the treated extract in step (3), and mea...
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