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Mycobacterium foruitum and application thereof

A technology of Mycobacterium fortuitum and seeds, which is applied in the fields of biotechnology and biochemical industry, can solve the problem of complex production process and low yield, and achieve the effects of simple preparation method, high efficiency and good application prospect.

Inactive Publication Date: 2016-08-10
SHANGHAI PESTICIDE RES INST +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The production process of the chemical synthesis method is complex and the yield is not high, so the production of 9α-hydroxy-androstenedione by the biological process fermentation method is the development direction

Method used

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  • Mycobacterium foruitum and application thereof
  • Mycobacterium foruitum and application thereof

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Experimental program
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Effect test

Embodiment 1

[0030] The wild-type Mycobacterium fortuitum of the present invention is collected from soil. Collected in Zhangjiajie, Hunan in August 2011.

[0031] Latin name: Mycobacterium foruitum

[0032] Deposit number: CGMCC No.9657

[0033] Deposit date: September 15, 2014.

[0034] Preservation institution: General Microorganism Center (CGMCC) of China Committee for Culture Collection of Microorganisms.

[0035] Address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.

[0036] The preservation condition of the above-mentioned Mycobacterium fortuitum is: vacuum freeze-drying (long-term preservation).

[0037] The culture conditions are as follows: a common culture medium for Mycobacterium fortuitum can be used, and cultured on a slant in an eggplant bottle at 25-30°C (preferably 28°C) for 6-7 days. Typical slant medium components: beef extract 0.2%, peptone 0.4%, glycerin 2%, agar powder 2% (pH natural).

Embodiment 2

[0039] The aforementioned Mycobacterium fortuitum can catalyze the production of 9α-hydroxyandrostenedione from phytosterols.

[0040] Place 20L of seed culture medium in a 35L seed tank, and insert the bacteria on the slant of an eggplant bottle (the area of ​​the slant is about 8cm×8cm). The seed medium contains 1% peptone, 0.3% beef extract, and 0.5% NaCl (both by mass percentage), and the pH is adjusted to 7.0.

[0041] The seed culture conditions are as follows: temperature 29±1°C, aeration ratio 1:0.6, rotation speed 480rpm, culture for 96 hours.

[0042] Take the seed culture medium, inoculate it into the fermentation broth according to 10% volume ratio, place it in a 50-liter fermenter for fermentation (30 L of charge), and use it to convert phytosterols. The pH of the fermentation medium is 8.5, and contains: soybean powder 0.1%, glycerol 1%, Na 2 HPO 4 0.84%, KH 2 PO 4 0.45%, NH 4 Cl0.2%, MgSO 4 0.03%, phytosterol 1%, Tween 80 0.1% (both are mass percentages),...

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Abstract

The invention relates to the fields of industrial microorganisms and fermentation technology, specifically, to mycobacterium foruitum having a catalytic function and an application of mycobacterium foruitum. The mycobacterium foruitum is provided with the accession number CGMCC No.9657, and can catalyze phytosterol to produce 9[alpha]-hydroxylandrostenedione. The conversation rate is over 95%, and the selectivity is 50-60%. The unit consumption in the production of 9[alpha]-hydroxylandrostenedione through utilization of mycobacterium foruitum is 2.2-2.5, and the main product is 9[alpha]-hydroxylandrostenedione. The preparation method is simple, is high in yield, and has bright application prospects.

Description

technical field [0001] The invention belongs to the field of biotechnology and biochemical industry, in particular to the field of industrial microbes and fermentation technology, in particular to a mycobacterium fortuitous with catalytic function and its application. Background technique [0002] Steroid hormone drugs refer to hormone drugs containing a steroid structure in their molecular structure, which is an important class of clinically important drugs, with strong pharmacological effects such as anti-infection, anti-allergy, anti-virus and anti-shock, and is an important part of the pharmaceutical industry. One of the key directions of development. These drugs mainly include adrenocortical hormones and sex hormones. Corticosteroid drugs used in clinic include cortisone acetate, hydrocortisone, dexamethasone acetate, fluocinonide and the like. [0003] The important raw materials for the production of steroid hormone drugs are phytosterols. 9α-hydroxy-androstenedion...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P33/16C12P33/02C12R1/33
Inventor 薛建萍姚再男唐璐敏范锦锦朱健丁林富卜桂琴
Owner SHANGHAI PESTICIDE RES INST
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