Preparation method of high-immune plasma protein powder

A technology for plasma protein powder and plasma is applied in the field of preparation of high-immunity plasma protein powder, which can solve the problems of reducing the biological value of products, difficult to kill pathogenic microorganisms, and high energy consumption, so as to enhance product functionality and ensure biological safety. the effect of improving protein content

Inactive Publication Date: 2016-08-17
HUAIBEI ENBI FEED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the high-temperature spray-drying process in the existing plasma protein powder processing technology is difficult to be replaced because it can realize the rapid disguised evaporation of water in the plasma solution and the effect of high-temperature killing of various pathogenic microorganisms in the plasma. The plasma solids content is low, about 6-8%. It is directly spray-dried without concentration, and the maximum inlet temperature can even reach 240°C. During the drying process, the immune-active globulin G in plasma proteins is seriously damaged, reducing the product biological value
Although high-activity plasma protein powder can be prepared by freeze-drying, the efficiency is low, the energy consumption is large, and the pathogenic microorganisms in the blood are difficult to kill. Therefore, it is important to study the IgG stabilization method in effective plasma protein and develop high-free plasma protein powder. Value

Method used

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  • Preparation method of high-immune plasma protein powder
  • Preparation method of high-immune plasma protein powder
  • Preparation method of high-immune plasma protein powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Raw material selection: fresh pig plasma from healthy pigs;

[0024] A. Take the freshly separated porcine plasma, pass the porcine plasma through a 150-mesh filter bag, and pump it into an inorganic membrane filtration system with a pore size of 0.22 μm, then pump the filtrate into an organic membrane concentration system with a molecular weight cut-off of 10000D, and circulate concentration, desalination and dehydration. Obtain a concentrated plasma solution with a solid content of 18%;

[0025] B. Pump the concentrated plasma liquid into a storage tank equipped with a stirrer, add reducing sugar according to the mass percentage of 2.0% of the total plasma, the added reducing sugar is maltodextrin sugar and glucose, and the mass ratio is 3:1, and then Stir and dissolve at 40°C and 180 rpm for 25 minutes to obtain sugar-added plasma;

[0026] C. Pump the sugar-added plasma liquid into the homogenizer, and under the condition of pressure 20MPa, homogenize under high pr...

Embodiment 2

[0046] Raw material selection: fresh blood plasma from healthy cattle

[0047] A. Take the freshly separated bovine plasma, pass the bovine plasma through a 150-mesh filter bag, and pump it into an inorganic membrane filtration system with a pore size of 0.22 μm, and then pump the filtrate into an organic membrane concentration system with a molecular weight cut-off of 20,000D for circular concentration, desalination and dehydration. Obtain a concentrated plasma solution with a solid content of 20%;

[0048] B. Pump the concentrated plasma liquid into a storage tank equipped with a stirrer, and add reducing sugar according to the mass percentage of 3.0% of the total plasma. The added reducing sugar is maltodextrin, glucose and fructose, and the mass ratio is 2:1 : 1, then stirred and dissolved at 45°C and 200 rpm for 30 minutes to obtain sugar-added plasma;

[0049] C. Pump the sugar-added plasma liquid into the homogenizer, under the condition of pressure 30MPa, high-pressur...

Embodiment 3

[0057] Raw material selection: Fresh blood plasma from healthy chicken

[0058] A. Take the freshly separated chicken plasma, pass the chicken plasma through a 150-mesh filter bag, pump it into an inorganic membrane filtration system with a pore size of 0.22 μm, and then pump the filtrate into an organic membrane concentration system with a molecular weight cut-off of 10000D, and circulate concentration for desalination and dehydration. Obtain a concentrated plasma solution with a solid content of 16%;

[0059] B. Pump the concentrated plasma liquid into a storage tank equipped with a stirrer, add reducing sugar according to the mass percentage of 1.0% of the total plasma, the added reducing sugar is maltodextrin, glucose and maltose, and its mass ratio is 8:1 : 1, then stirred and dissolved at 30°C and 120 rpm for 15 minutes to obtain sugar-added plasma;

[0060] C. Pump the sugar-added plasma liquid into the homogenizer, and under the condition of pressure 10MPa, homogenize...

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Abstract

The invention relates to a preparation method of high-immune plasma protein powder. According to the method, fresh plasma liquid of healthy slaughtered livestock and poultry as a raw material, a process method including the steps of inorganic membrane filtering, organic membrane concentrating, reducing sugar adding, high-pressure homogenizing and spray drying is adopted, the prepared high-immune plasma protein powder basically has no blood smell, the protein content of the high-immune plasma protein powder reaches 75% or above, and the immune globulin G content of the high-immune plasma protein powder is increased by 40% or above compared with common plasma protein powder; reducing sugar and plasma immune globulin particles are prepared through reducing sugar adding, homogenizing and centrifugal atomization treating, damage of the high temperature to immune globulin in the spray drying process is reduced through the affinity effect of rich hydrophilic hydroxyl groups in reducing sugar to water, therefore, the activity of the immune globulin is guaranteed, and preparation of the high-immune plasma protein powder is achieved.

Description

technical field [0001] The invention belongs to the technical field of livestock and poultry blood processing and utilization, and in particular relates to a preparation method of high-free plasma protein powder. Background technique [0002] my country is the largest pig production country in the world. Every year, tens of millions of pigs are killed or eliminated due to various reasons such as diseases. Among them, the number of piglets under 30kg culled and killed accounted for more than half of the culled mortality rate in the entire breeding period. The reason for this phenomenon is The main reason is that the body resistance of piglets is weak and the incidence rate is high, which leads to a decrease in the survival rate of piglets, a reduction in feed remuneration, slow growth, and stagnant growth and development (stiff pigs), which seriously threatens the healthy development of the pig industry. Tens of billions of dollars have been invested in feeding, management and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/06A23K50/30A23K20/147
CPCA23J1/06
Inventor 蔡克周程榆茗王俊武吴明文陈炎
Owner HUAIBEI ENBI FEED
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