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Use of berberine in the preparation of hedgehong signaling pathway inhibitors

A signaling pathway, berberine technology, applied in the field of berberine preparation of Hedgehong signaling pathway inhibitors, can solve problems such as tumors that have not yet been seen, and achieve the effects of being beneficial to clinical application, inhibiting tumor growth, and low price

Inactive Publication Date: 2019-04-05
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there has been no research report on BBR's definite inhibition of tumor growth

Method used

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  • Use of berberine in the preparation of hedgehong signaling pathway inhibitors
  • Use of berberine in the preparation of hedgehong signaling pathway inhibitors
  • Use of berberine in the preparation of hedgehong signaling pathway inhibitors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: The specific inhibitory effect of BBR on Hedgehog signaling pathway in vitro

[0025] 1. The inhibitory effect of BBR on the Hedgehog signaling pathway in vitro:

[0026] 1): Gli-luciferase dual luciferase reporter gene test

[0027] NIH3T3 (American Type Culture Collection) cells were treated with Dulbecco's modified Eagle's medium (DMEM) (GIBCO) containing 10% (v / v) newborn bovine serum (GIBCO), penicillin / streptomycin (Hyclone), 37°C, 5% CO2 Constant temperature culture. Inoculate 3×10 4 cells in a 48-well plate. After 24 hours, Gli-dependent firefly luciferase reporter and TK-Renillaluciferase reporter vectors were transfected with transfection reagent lipo2000 (Invitrogen). 48 hours after transfection, the cells were treated with medium containing 0.5% newborn bovine serum (GIBCO), SAG (100 nM) or Shh-N conditioned medium (ShhN-CM) and BBR prepared in different concentrations. The reaction was terminated after 36 h. Luciferase activity was detected...

Embodiment 2

[0042] Example 2: In vivo anti-tumor effect experiment of BBR on Hh-dependent medulloblastoma

[0043] 1. BBR inhibits the expression of Gli1 mRNA in medulloblastoma cells

[0044] Select a well-grown Ptch + / - P53 - / - Tumor cells (MB cells), Neurobasal-A Medium (GIBCO), B-27 Supplement (GIBCO), Sodium Pyruvate (GIBCO), L-Glutamine, isolated from spontaneously grown medulloblastoma tissue of C57B / L mice (GIBCO), penicillin / streptomycin (Hyclone), 37 ° C, 5% CO2 constant temperature culture, inoculated 5 × 10 6 After 24 hours, the cells were added with different concentrations of BBR, and the total RNA was extracted with Trizol after 24 hours. The endogenous gene GUSB was used as a reference, and the reverse transcription kit ( RT Master Mix, Takara) reverse transcription, with Premix Ex Taq TM (Tli RNaseH Plus) (Takara) was quantified in real time according to the instructions, using the iCycler iQsystem (Bio-Rad) real-time fluorescent quantitative PCR instrument to quan...

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PUM

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Abstract

The present invention relates to the fields of biology, molecular biology and tumor pharmacology, and in particular relates to application of berberine to the preparation of a Hedgehong signaling pathway inhibitor. The present invention provides application of berberine (hereinafter referred to as BBR) as the Hedgehog pathway inhibitor, particularly obvious inhibition effect in the anti-medulloblastoma growth. The BBR can be used alone, or prepared into an anticancer drug as an active ingredient, wherein the drug has dosage forms of tablets, capsules, oral liquid and liposome, so as to expand application field of BBR.

Description

technical field [0001] The invention belongs to the fields of cell biology, molecular biology and tumor pharmacology, and relates to a new medicinal application of berberine (BBR for short), in particular to the application of berberine in the preparation of Hedgehong signaling pathway inhibitors. Background technique [0002] The prior art discloses that Hedgehog (Hh) is a segment polarity gene discovered by Christianey and Eric in large-scale gene mutation screening in Drosophila in 1980. Usually, the Hh signaling pathway is involved in the formation of somites and appendages of insect embryos, and at the same time plays a key role in the differentiation of mammalian neural tubes, as well as participating in the self-renewal and repair of cells; studies have shown that continuous abnormal activation of Hh signaling pathways has a carcinogenic effect . In particular, the recent research on the Hh signaling pathway has shown that the abnormal activation of the Hh signaling ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/4375A61P35/00
Inventor 谭文福王娟彭元求刘原杨君
Owner FUDAN UNIV