Biological active peptide for promoting growth of piglet and preparation method and application thereof

A bioactive peptide, piglet technology, applied in biochemical equipment and methods, microorganism-based methods, applications, etc., can solve the problems of imperfect digestive gland physiology, imperfect body temperature regulation, and lack of innate immunity, etc. Achieve the effect of overcoming hormone residues, promoting growth and development, and increasing daily weight gain

Active Publication Date: 2016-08-17
湖北博大生物股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The growth and development of piglets is a key link related to pig production. Due to the unique digestive physiological structure of piglets, their digestive organs are not perfect, the physiological functions of digestive glands are not perfect, they lack congenital immunity, and their body temperature regulation function is not perfect. Stress resistance is poor, and piglets have a high incidence of various diseases and low survival rates under normal feeding conditions

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  • Biological active peptide for promoting growth of piglet and preparation method and application thereof

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preparation example Construction

[0015] The third aspect of the present invention provides a method for preparing the above-mentioned bioactive peptide for promoting growth of piglets, comprising the following steps:

[0016] A. Culture after screening Enterococcus lactic acid, Bacillus licheniformis, Bacillus coagulans, Bacillus pumilus and Candida utilis respectively;

[0017] B. Enterococcus lactic acid, Bacillus licheniformis, Bacillus coagulans, Bacillus pumilus and Candida utilis obtained in step A are cultured and fermented, the supernatant of the fermentation broth is extracted, concentrated, dried, separated and purified to obtain bioactive peptides.

[0018] The biologically active peptides for promoting the growth and development of piglets are produced under the synergy of various enzyme-producing probiotics. Improve the enzyme production characteristics, enzyme production and enzyme activity of probiotics through artificial mutagenesis, and carry out synchronous compound culture of Enterococcus l...

Embodiment 1

[0033] All bacterial strains adopted in this embodiment are microbial strains that can be added in feed, and Enterococcus lactic acid, Bacillus licheniformis, Bacillus coagulans, Bacillus pumilus, and Candida utilis are produced by the Agricultural Microbiology National Provided by key laboratories, Enterococcus lactis, Bacillus licheniformis, Bacillus coagulans, Bacillus pumilus and Candida utilis purchased on the market can also achieve very similar effects.

[0034] One, the preparation method of the bioactive peptide that promotes the growth of piglets of the present embodiment comprises the following steps:

[0035] Step 1: Cultivate strains, and optimize each strain by mutation breeding technology.

[0036] Under an ultraviolet lamp with a power of 20W and an irradiation distance of 30cm, 2mL of the preserved bacterial suspensions of Enterococcus lactis, Bacillus licheniformis, Bacillus coagulans, Bacillus pumilus, and Candida utilis were separated from the casein separa...

Embodiment 2

[0057] All bacterial strains adopted in this embodiment are microbial strains that can be added in feed, and Enterococcus lactis, Bacillus licheniformis, Bacillus coagulans, Bacillus pumilus, and Candida utilis are all purchased in the market , strains from different manufacturers can achieve similar results.

[0058] One, the preparation method of the bioactive peptide that promotes the growth of piglets of the present embodiment comprises the following steps:

[0059] Step 1: Cultivate strains, and optimize each strain by mutation breeding technology.

[0060] Under an ultraviolet lamp with a power of 20W and an irradiation distance of 30cm, 2mL of the preserved bacterial suspensions of Enterococcus lactis, Bacillus licheniformis, Bacillus coagulans, Bacillus pumilus, and Candida utilis were separated from the casein separation and screening medium. On a sterile plate, irradiate for 2-3 minutes to culture and count, observe the transparent circle on the casein separation an...

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Abstract

The invention provides biological active peptide for promoting growth of a piglet. The biological active peptide comprises an amino acid sequence shown in SEQ IDNo.1, the absorption and utilization rate of nutrient substances in feed for the piglet can be obviously increased through the biological active peptide, growth and development of the piglet can be promoted, the morbidity can be reduced, and the survival rate of the piglet can be increased. Furthermore, the preparation method of the biological active peptide for promoting growth of the piglet and application of the biological active peptide to promoting growth of the piglet and reducing the death rate of the piglet are provided. The biological active peptide for promoting growth and development of the piglet is generated under the synergistic effect of multiple kinds of enzyme production probiotics and is applied to the piglet, constructive metabolism and lipolysis of the organism are promoted, protein synthesis is promoted, and the aims of promoting growth of the piglet and increasing the growth speed of the piglet are achieved. The function of promoting growth and development of the piglet is achieved by adjusting the hormone level of an endocrine system, the problem of hormone residues is solved, and the aim of healthy and environment-friendly breeding is achieved.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a biologically active peptide that can effectively promote the growth and development of piglets, increase their growth speed and promote the growth of piglets, and its preparation method and application. Background technique [0002] The growth and development of piglets is a key link related to pig production. Due to the unique digestive physiological structure of piglets, their digestive organs are not perfect, the physiological functions of digestive glands are not perfect, they lack congenital immunity, and their body temperature regulation function is not perfect. Stress resistance is poor, and piglets have a high incidence of various diseases and a low survival rate under normal feeding conditions. Improving the absorption and utilization rate of nutrients in the feed by piglets, promoting the growth and development of piglets, reducing the inci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08C12P39/00C12P21/02A23K20/147A23K50/30C12R1/46C12R1/10C12R1/07C12R1/72
Inventor 孙国平吴正荣范博文陈义杰李亮
Owner 湖北博大生物股份有限公司
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