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A TLC identification method for Chuantongpi formula granules

A technology for identification of formula particles and thin layers, which is applied to measuring devices, instruments, scientific instruments, etc., to achieve the effects of reducing pollution, protecting health, and increasing specificity

Active Publication Date: 2017-08-29
SICHUAN NEO GREEN PHARMA TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Inability to perform TLC identification with complete certainty

Method used

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  • A TLC identification method for Chuantongpi formula granules
  • A TLC identification method for Chuantongpi formula granules
  • A TLC identification method for Chuantongpi formula granules

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Embodiment Construction

[0041] In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with specific embodiments, but it should not be understood that the scope of the above-mentioned subject of the present invention is limited to the following embodiments.

[0042] 1. Instruments and reagents

[0043] Instruments: Thin-layer automatic imager (CAMAG TLC VISUALIZER), linamat5-CAMAG semi-automatic spotting instrument, one-tenth balance (Sartorius BS110S), KQ-250B ultrasonic cleaner (Kunshan Ultrasonic Electronics Co., Ltd.), drug stabilization Test chamber (Shanghai Yiheng Scientific Instrument Co., Ltd.), Silicone G thin layer plate (Tianjin Silida Technology Co., Ltd., cuttable plate, batch number: 130508), Silicone G thin layer plate (Qingdao Yuminyuan Silica Gel Reagent Factory, Glass plate, batch number: 141201), silica gel G thin layer plate (Qingdao Haiyang Chemical Plant branc...

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Abstract

The invention relates to the technical field of pharmaceutical analysis and specifically relates to a thin-layer identification method for septemlobate kalopanax bark formula granules. The thin-layer identification method comprises the following steps: taking 0.5g granules and grinding; adding methyl alcohol and then performing ultrasonic processing; filtering; adding hydrochloric acid into filtrate and then heating and refluxing for 1 hour; drying by distillation; adding 1ml methyl alcohol into residue, dissolving and taking as a test solution; taking a defined amount of oleanolic acid and hederagenin reference substance; adding the methyl alcohol and preparing into a reference substance solution; respectively sucking 3mu l of the two solutions and respectively dipping the solutions to a same silica gel G thin layer plate; taking cyclohexane-ethyl acetate-glacial acetic acid as a developing solvent; developing, taking out and drying in the air; spraying ethanol sulfate solution; heating at 105 DEG C till the spot developing is clear; placing under an ultraviolet lamp for inspecting; in a test chromatography, developing the fluorescent spots in same color on the position corresponding to the reference substance chromatography. The result thin-layer chromatography spot is clear and the repeatability is excellent.

Description

Technical field [0001] The invention relates to the technical field of drug analysis, in particular to a thin-layer identification method of Chuan Tong Pi formula particles. Background technique [0002] Chuan Tong Bark is the dry bark of Araliaceae thorn tree, or thorn tree. The bark can be harvested throughout the year, stripped of the bark, dried in the sun, can cure rheumatism, dredge collaterals, and relieve pain. Used for rheumatoid arthritis. Lumbar and knee pain; externally used for bruises. It often grows in mountainous forests and is produced in Anhui, Hubei, Hunan or Guangxi. [0003] There are few domestic reports on the related research of Chuan Tong Bark. At present, there are no thin-layer identification items for Chuan Tong Bark in the current pharmacopoeias and the standards of Chinese medicinal materials in various provinces, and Chuan Tong Bark formula granules also lack thin-layer identification items. Only a few R&D units have them. Its own method of identi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/90G01N30/06
CPCG01N30/06G01N30/90G01N2030/062
Inventor 周厚成胡昌江周维冯健李文兵
Owner SICHUAN NEO GREEN PHARMA TECH DEV