Fusion protein of mycoplasma pneumonia protein epitope and preparation and application thereof

Abstract

The invention discloses a fusion protein of a mycoplasma pneumonia protein epitope and preparation and application thereof, and relates to the fields of genetic engineering technologies, vaccines and diagnosis reagents. Amino acid sequences of a mycoplasma pneumoniae P116 protein, a P1 protein and a P30 protein are analyzed through a computer, mycoplasma pneumoniae P116 protein fragments, namely from the 261st amino acid to the 466th amino acid containing the strong epitope, mycoplasma pneumoniae P1 protein fragments, namely from the 1125th amino acid to the 1395th amino acid containing the strong epitope and mycoplasma pneumoniae P30 protein fragments, namely from the 107th amino acid to the 161st amino acid containing the strong epitope are screened out. Series connection gene sequences of novel mycoplasma pneumoniae P116 protein fragments, mycoplasma pneumoniae P1 protein fragments and mycoplasma pneumoniae P30 protein fragments are synthesized chemically, three gene fragments are connected in series, the fusion protein of P116 protein fragments, P1 protein fragments and P30 protein fragments is expressed by means of the genetic engineering technology, the three protein fragments are connected through glycine-glycine-serine, and 538 amino acids are formed in the overall length of the fusion protein.

Description

technical field [0001] The fusion protein of the mycoplasma pneumoniae protein antigen epitope and its preparation and application in the present invention relate to the fields of genetic engineering technology, vaccines and diagnostic reagents. The amino acid sequence of the mycoplasma pneumoniae P116 protein, P1 protein and P30 protein is screened out by computer. The P116 protein fragment of Mycoplasma pneumoniae with a strong antigenic epitope, the P1 protein fragment of Mycoplasma pneumoniae with a strong antigenic epitope, and the P30 protein fragment of Mycoplasma pneumoniae with a strong antigenic epitope. Select codons favored by both eukaryotes and prokaryotes, chemically synthesize a new tandem gene sequence of Mycoplasma pneumoniae P116 protein fragment, P1 protein fragment and P30 protein fragment, and express P116 protein fragment, P1 protein fragment and P30 protein fragment by genetic engineering technology The fusion protein, the three protein fragments are co...

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Problems solved by technology

[0005] Mycoplasma pneumoniae can be cultured in vitro with PPLO medium, so the immunofluorescence method and enzyme-linked immunoassay method for detecting Mycoplasma pneumoniae antibodies have been established by using the cultured Mycoplasma pneumoniae, but it is difficult to mass-produce the antigen detection antibody using the cultured Mycoplasma pneumoniae False positives are generated, and the development of specific genetic recombinant antigens is t

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