New fermentation type coffee beans and preparation method thereof
A coffee bean and fermented technology, which is applied in green coffee processing, etc., can solve the problems that coffee cannot meet the needs of consumers, the quality of coffee beans is uneven, and the quality of coffee declines, so as to achieve low cost of process equipment and enhanced functionality , The effect of reducing the content of caffeine
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Embodiment 1
[0032] The preparation method of the fermented coffee beans of this embodiment includes the following steps:
[0033] (1) Preparation of natto bacteria liquid seeds: use conventional methods to screen and activate natto bacteria, then inoculate the activated natto bacteria into the soybean powder liquid medium, and cultivate it in an incubator at 37°C for 24 hours. Obtain the liquid seed of Natto in the log phase, the number of Natto is about 10 9 cfu / mL;
[0034] (2) Coffee bean pretreatment: Sterilize fresh coffee beans by dry heat at 160°C and cool to 25°C naturally;
[0035] (3) Fermentation of coffee beans: place the sterilized coffee beans in a fermentation basin, inoculate the liquid seeds of natto bacteria prepared in step (1), inoculate 100ml of liquid natto seeds per kilogram of coffee beans, and the coffee beans after inoculation Control the water content at about 50%, and ferment for 24h at 37℃;
[0036] (4) Roasting of coffee beans: the fermented coffee beans are roasted...
Embodiment 2
[0038] The preparation method of the fermented coffee beans of this embodiment includes the following steps:
[0039] (1) Preparation of natto bacteria liquid seeds: use conventional methods to screen and activate natto bacteria, then inoculate the activated natto bacteria into the soybean powder liquid medium, and cultivate it in an incubator at 37°C for 24 hours. Obtain the liquid seed of Natto in the log phase, the number of Natto is about 10 9 cfu / mL;
[0040] (2) Coffee bean pretreatment: Sterilize fresh coffee beans by dry heat at 160°C and cool to 25°C naturally;
[0041] (3) Fermentation of coffee beans: place the sterilized coffee beans in a fermentation basin, inoculate the liquid seeds of bacteria natto prepared in step (1), inoculate 120ml of liquid seeds of bacteria natto per kilogram of coffee beans, and the coffee beans after inoculation Control the water content at about 55%, and ferment at 37°C for 24 hours;
[0042] (4) Roasting of coffee beans: the fermented coffee...
Embodiment 3
[0044] The preparation method of the fermented coffee beans of this embodiment includes the following steps:
[0045] (1) Preparation of natto bacteria liquid seeds: use conventional methods to screen and activate natto bacteria, then inoculate the activated natto bacteria into the soybean powder liquid medium, and cultivate it in an incubator at 37°C for 24 hours. Obtain the liquid seed of Natto in the log phase, the number of Natto is about 10 9 cfu / mL;
[0046] (2) Coffee bean pretreatment: Sterilize fresh coffee beans by dry heat at 160°C and cool to 25°C naturally;
[0047] (3) Fermentation of coffee beans: place the sterilized coffee beans in a fermentation basin, inoculate the liquid seeds of natto bacteria prepared in step (1), inoculate 80ml of liquid natto seeds per kilogram of coffee beans, and the coffee beans after inoculation Control the water content at about 45%, and ferment for 24h at 37℃;
[0048] (4) Roasting of coffee beans: the fermented coffee beans are roasted ...
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