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A kind of extraction method of salidroside

A salidroside and extraction method technology, applied in chemical instruments and methods, organic chemistry, sugar derivatives, etc., can solve the problems of complex operation process and low yield, and achieve the effect of improving the extraction rate

Active Publication Date: 2018-11-06
陕西笃诚医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Patent CN021004897 discloses a method for extracting salidroside through two high-speed countercurrent chromatography, the purity of the extracted product is high, but the yield is low
[0005] Patent CN201310463903.9 discloses a method for extracting salidroside from Rhodiola rosea, which adopts the combination of macroporous resin purification and simulated moving bed purification for separation and purification. Although the purity of the extracted product is high, the operation process More complex and lower yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] After crushing 1 kg of dried Rhodiola rosea, add 8 times the weight of sodium citrate / citric acid buffer solution with pH=3.8, then add 0.5% cellulase and pectinase (1:1), at 45°C, After 2 hours of enzymatic hydrolysis, transfer to a continuous countercurrent extraction tank, add 4kg of water, reflux extraction, filter, and concentrate to obtain crude salidroside extract; add water to dissolve, absorb with macroporous adsorption resin AB-8, and use 40% Eluted with ethanol, collected the eluate, concentrated under reduced pressure to obtain crude salidroside; purified by high-speed countercurrent chromatography, collected components containing salidroside, evaporated to dryness under reduced pressure, and obtained pure salidroside 13.2 g, HPLC: 99.15%; wherein the stationary phase and the mobile phase in the high-speed countercurrent chromatography are prepared according to the following method: cyclohexane: n-butanol: methanol: water = 3:2.5:1.5:3.5 ratio of cyclohexane ...

Embodiment 2

[0026] After crushing 1 kg of dried Rhodiola rosea, add 5 times the weight of PH=4.0 sodium citrate / citric acid buffer solution, then add 0.8% cellulase, hemicellulase and pectinase (1:1:1 ), at 50°C, after 2 hours of enzymatic hydrolysis, transfer to a continuous countercurrent extraction tank, add 5kg of water, reflux extraction, filter, concentrate to obtain crude salidroside extract; add water to dissolve, and use macroporous adsorption resin HPD100 Adsorbed, eluted with 35% ethanol, collected eluate, concentrated under reduced pressure to obtain crude salidroside; purified by high-speed countercurrent chromatography, collected components containing salidroside, and evaporated to dryness under reduced pressure to obtain Rhodiola Tianglycoside pure product 13.5g, HPLC: 99.53%; Among them, the stationary phase and mobile phase in high-speed countercurrent chromatography are prepared according to the following method: cyclohexane: n-butanol: methanol: water = 3: 2.5: 1.5: 3.5 ...

Embodiment 3

[0028] After crushing 1 kg of dried Rhodiola rosea, add 10 times the weight of sodium citrate / citric acid buffer solution with pH=4.5, and then add 1% hemicellulase and pectinase (1:1), at 55°C , after 1.5h of enzymolysis, transfer to continuous countercurrent extraction tank, add 2kg of water, reflux extraction, filter, concentrate to obtain crude salidroside extract; add water to dissolve, absorb with macroporous adsorption resin D-101, use Eluted with 45% ethanol, collected the eluate, concentrated under reduced pressure to obtain crude salidroside; separated by high-speed countercurrent chromatography, collected components containing salidroside, and evaporated to dryness under reduced pressure to obtain pure salidroside Product 12.9g, HPLC: 99.37%; wherein the stationary phase and the mobile phase in the high-speed countercurrent chromatography are prepared according to the following method: Cyclohexane: n-butanol: methanol: water=3:2.5:1.5:3.5 ratio cyclohexane Hexane, n...

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PUM

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Abstract

The invention discloses an extracting method for salidroside. According to the extracting method, the salidroside is extracted by combining biological enzyme with a continuous counter-current extracting method; meanwhile, macroporous adsorption resin is combined with high-speed counter-current chromatography to separate and purify the salidroside. The extracting method is an salidroside extracting method which is high in extracting efficiency, is obvious in separating and purifying effect, is high in purity (higher than 99%) of an extracted product and is high in yield, and has a good application prospect.

Description

technical field [0001] The invention belongs to the field of natural product extraction, and relates to a method for extracting salidroside, in particular to a production method for separating and purifying salidroside with macroporous adsorption resin combined with high-speed countercurrent chromatography. Background technique [0002] Rhodiola rosea is a perennial herb or shrub of the Sedum family, mainly containing phenylpropanoids and flavonoids. Among them, salidroside is the main active ingredient in Rhodiola rosea, and it is also the most important indicator for evaluating Rhodiola rosea and its extracts. Modern pharmacological studies have shown that salidroside has the functions of eliminating fatigue, anti-hypoxia, anti-tumor, anti-aging, and anti-microwave radiation. [0003] At present, scholars at home and abroad have conducted in-depth research on the chemical composition, pharmacological effects, and structural identification of the main active ingredients of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/18C07H1/08
CPCC07H1/08C07H15/18
Inventor 陈阿梅
Owner 陕西笃诚医药科技有限公司