Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for controlling mouse sex based on inhibiting expression of Dby genes

A gene expression and mouse technology, applied in the field of genetic engineering, can solve problems such as loss of reproductive ability, loss of reproductive ability, and failure to apply

Inactive Publication Date: 2016-09-07
SOUTH CHINA AGRI UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mice knocked out of the Sry gene will show sex reversal, such as female-like appearance and female-like internal genitalia, etc., but their reproductive ability is lost; A piece of DNA, some mice have sex reversal, there is no difference in growth performance (size, weight) and mating method compared with normal XY male mice, but the same fecundity loss
Therefore, although knocking out or knocking in the Sry gene can change the sex of animals, the loss of reproductive function makes this technology not applicable in production.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for controlling mouse sex based on inhibiting expression of Dby genes
  • Method for controlling mouse sex based on inhibiting expression of Dby genes
  • Method for controlling mouse sex based on inhibiting expression of Dby genes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0048] Below in conjunction with accompanying drawing, invention is described in further detail.

[0049] The pGLV / H3 / GFP+Puro Vector vector involved in the present invention was purchased from Shanghai Jima Pharmaceutical Technology Co., Ltd.; the Dby gene sequence was derived from GenBank accession number: NM-012008.2; the internal reference gene GAPDH gene sequence was derived from: GenBank accession number: NM_001289726 .1; 293T and MC3T3-E1 cells were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences. 1. Construction of anti-Dby shRNA lentiviral expression vector

[0050] Design 4 target sites for the Dby gene according to the siRNA design principle, design and synthesize the DNA insert sequence of the corresponding shRNA according to the target site, the shRNA name of the 4 target sites, the corresponding DNA insert sequence and the NC negative control sequence are shown in the table below 1:

[0051]

[0052]

[0053] The DNA insertion seq...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for controlling mouse sex based on inhibiting expression of Dby genes. According to the method, the expression of the Dby genes is inhibited by virtue of an RNA interference technology, so that mouse sex control is achieved by reducing the birth ratio of male mice. Specifically, the method, by inhibiting the expression of the Dby genes on Y chromosomes, can achieve the purpose of inhibiting the function of the Dby genes; and with the loss of the function of the Dby genes which are important genes for early embryonic development, the development of the Y chromosomes and male zygotes is inhibited, so that the birth ratio of the male mice is reduced; therefore, the purpose of controlling the mouse sex is achieved.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a method for sex control of mice based on inhibiting Dby gene expression. Background technique [0002] Sex control refers to a biotechnology that artificially intervenes in the reproductive process of animals so that female animals can produce offspring that meet a specific sex. Animal sex control technology is of great significance in preventing human genetic diseases, improving livestock production capacity, animal genetic breeding and protection of endangered and rare animals. [0003] At present, there are many methods for animal sex control, but none of them are suitable for large-scale promotion and application: [0004] 1. Separation of X and Y sperm [0005] Sperm separation methods include chromatography (difference in DNA content of X and Y sperm), electrophoresis (difference in charge carried by X and Y sperm), Percoll density gradient centrifugation (di...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/867C12N15/877C12N5/10
CPCC07K14/47C12N15/867C12N15/877C12N2800/107
Inventor 吴珍芳刘德武李崇杨晓峰李紫聪郑恩琴
Owner SOUTH CHINA AGRI UNIV