Application of serum exosomes miR-9-3p and miR-124-3p as diagnosis markers of acute cerebral infarction

A diagnostic marker, exosome technology, applied in the field of biotechnology and medicine, can solve the problem of lack of tissue specificity

Active Publication Date: 2016-09-07
广州爱索达生物医药技术有限公司
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  • Abstract
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Problems solved by technology

But they also lack tissue specificity, miR-145 is mainly distributed in vascular smooth muscle cells [Creemers EE, Tijsen AJ, Pinto YM. Circulating microRNAs: novel biomarkers and extracellular communicators in cardiovascular disease? [J]. Circ Res, 2012, 110(3): 483-495.] and miRNA-210 is an oxygen-sensitive microRNA [Creemers EE, Tijsen AJ, Pinto YM. Circulating microRNAs: novel biomarkers and extracellular communicators in cardiovascular disease? [J].Circ Res,2012,110(3):483-495.]

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  • Application of serum exosomes miR-9-3p and miR-124-3p as diagnosis markers of acute cerebral infarction
  • Application of serum exosomes miR-9-3p and miR-124-3p as diagnosis markers of acute cerebral infarction
  • Application of serum exosomes miR-9-3p and miR-124-3p as diagnosis markers of acute cerebral infarction

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Embodiment

[0017] 1. 1. Research objects: 32 patients with acute cerebral infarction who were hospitalized in the Neurology Department of the Affiliated Hospital of Nantong University from January 2015 to December 2015 were selected as the research group. Among them, there were 20 males and 12 females. Aged 40 to 76 years old, with an average of (61.3±10.3) years old. All of them were confirmed by cranial CT or MRI examination, and met the diagnostic criteria for cerebral infarction revised by the Fourth National Cerebrovascular Disease Academic Conference. The onset time was 48-72 hours, and the median (interquartile range) of NIHSS score was 8 (8-12). Malignant tumors, myocardial infarction or heart failure, blood, endocrine, metabolic or digestive system diseases, malnutrition, severe lung infection, liver and kidney insufficiency, and other diseases of the nervous system are excluded. 33 healthy volunteers confirmed by the physical examination center of the hospital during the same...

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Abstract

The invention discloses an application of serum exosomes miR-9-3p and miR-124-3p as diagnosis markers of acute cerebral infarction, wherein sequences of the miR-9-3p and miR-124-3p are shown as AUAAAGCUAGAUAACCGAAAGU and UAAGGCACGCGGUGAAUGCC. The exosomes in serum are precipitated by virtue of a high-molecular polyme, and the expression and differences of the brain tissue specific miR-9-3p and miR-124-3p in serum exosomes of a patient with stroke and a healthy person are analyzed by virtue of a real-time fluorescent quantitative polymerase chain reaction (PCR) technology. In the case of acute stroke, the brain tissue specific miR-9-3p and miR-124-3p enter peripheral blood in the form of the exosomes, with a level obviously increased in comparison with a control group, and expression quantities are positively correlated to a degree of brain injury; therefore, the miR-9-3p and miR-124-3p can be used as the markers for judging cerebral ischemic injury and degree.

Description

technical field [0001] The invention belongs to the fields of biotechnology and medicine, and in particular relates to the application of serum exosome miR-9-3p and miR-124-3p as diagnostic markers for acute cerebral infarction. Background technique [0002] Stroke is one of the diseases with high morbidity and mortality worldwide, and is the leading cause of adult disability. It has the characteristics of "five highs", that is, high morbidity rate, high mortality rate, high disability rate, high recurrence rate, and high cost of diagnosis and treatment, causing a heavy economic burden to the country and patients' families. Ischemic stroke (IS) accounts for 80% of all strokes [Donnan GA, Fisher M, Macleod M, et al. Stroke [J]. Lancet, 2008, 371 (9624): 1612-1623.], Thrombolytic therapy using tissue plasminogen activator (t-PA) to recanalize the occluded blood vessels to rescue the ischemic penumbra is currently the most effective treatment, but this is limited by the narrow...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 季秋虹季煜华季菊玲
Owner 广州爱索达生物医药技术有限公司
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