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A method for micropropagation of Wutun Yang

A technology of micropropagation and stem segments, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as difficulty in achieving large-scale production, restrictions on large-scale reproduction, and long cutting propagation cycle, so as to reduce the area occupied by production , Reduce the time required for seedling cultivation and shorten the cultivation time

Inactive Publication Date: 2018-05-04
DALIAN NATIONALITIES UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the expansion and promotion of Wutun Yang is mainly through cutting propagation, the propagation efficiency is low, the cutting propagation cycle is long, and its large-scale propagation is limited by many factors such as seasons. The method of tissue culture can solve some problems in the cutting propagation process. problems and can obtain a large number of tissue culture seedlings in a short period of time, but conventional tissue culture is time-consuming and labor-intensive, making it difficult to achieve large-scale production

Method used

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  • A method for micropropagation of Wutun Yang
  • A method for micropropagation of Wutun Yang
  • A method for micropropagation of Wutun Yang

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0051] (1) Selection of explants

[0052] Choose robust growth, no disease and insect pests Yang Yang stem section as explants;

[0053] (2) disinfection of explants

[0054] The above-mentioned stem sections are cut into sections every 1-2 buds (approximately every section is 2-3cm), and the cut-off stem sections are washed with water for 30min, and the stem sections are put into a sterile ultra-clean bench, and washed with 75vt% Soak in alcohol for 30s, rinse with sterile water for 3 times, then soak with 0.1wt% mercuric chloride for 5 minutes, and rinse with sterile water for 5 times;

[0055] (3) Induction of explant buds

[0056] The above-mentioned explants to be used were inserted into the induction medium for bud induction.

[0057] Induction medium formula: MS medium, pH 5.80, cultivated under light at 25°C for 8 days to obtain clustered buds;

[0058] (4) Bud differentiation and proliferation culture

[0059] The clustered buds obtained in step (3) are cut off a...

Embodiment 3

[0068] (1) Selection of explants

[0069] Choose robust growth, no disease and insect pests Yang Yang stem section as explants;

[0070] (2) disinfection of explants

[0071] The above-mentioned stem section is cut off every 1-2 buds (approximately every section is at 2-3cm), the cut-off stem section is rinsed with water for 30min, and then the stem section is put into a sterile ultra-clean bench and washed with 75vt% Soak in alcohol for 30s, rinse with sterile water for 3 times, then soak with 0.1wt% mercuric chloride for 5 minutes, and rinse with sterile water for 5 times;

[0072] (3) Induction of explant buds

[0073] The above-mentioned explants to be used were inserted into the culture medium for bud induction.

[0074] Bud induction medium formula: MS medium, pH 5.84, cultivated under light at 28°C for 15 days to obtain clustered buds;

[0075] (4) Bud differentiation and proliferation culture

[0076] The induced shoots were excised and inserted into a differentia...

Embodiment 4

[0085] (1) Selection of explants

[0086] Choose robust growth, no disease and insect pests Yang Yang stem section as explants;

[0087] (2) disinfection of explants

[0088] The above-mentioned stem sections are cut into sections every 1-2 buds (approximately every section is 2-3cm), and the cut-off stem sections are washed with water for 30min, and the stem sections are put into a sterile ultra-clean bench, and washed with 75vt% Soak in alcohol for 30s, rinse with sterile water for 3 times, then soak with 0.1wt% mercuric chloride for 5 minutes, and rinse with sterile water for 5 times;

[0089] (3) Induction of explant buds

[0090] The above-mentioned explants to be used were inserted into the induction medium for bud induction.

[0091] Induction medium formula: MS medium, adjust the pH to 5.80, culture at 25°C for 8 days under light to obtain clustered buds;

[0092] (4) Bud differentiation and proliferation culture

[0093] The clustered buds obtained in step (3) ar...

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Abstract

The invention relates to a plant micropropagation method, in particular to a populus wutunensis micropropagation method. The method mainly includes the following steps of selection of an explant, sterilization of the explant, bud induction, bud initiation and enrichment culture of the explant, steam put-forth and rooting culture, seedling hardening and transplanting. A culture medium formula used in bud initiation and enrichment culture is prepared in the steps that MS serves as a basic culture medium, 0.4-0.6 mg / L of 6-BA (6-benzyladenine), 0.1-0.3 mg / L of NAA (naphthylacetic acid), 7-10 g / L of agar and 30-50 g / L of cane sugar are added, the materials are cultured for 12-17 days under light at pH of 5.80-5.84, and cluster buds are obtained. A stem serves as the explant, axillary bud germinating is induced through hormone, buds are subjected to enrichment culture, a large number of vegetative propagation seedlings can be obtained, and the propagation coefficient of populus wutunensis tissue culture seedlings can be improved by ten times.

Description

technical field [0001] The invention belongs to the technical field of plant micropropagation production, and relates to a method for micropropagation of Populus wutun. Background technique [0002] my country is one of the countries with large saline-alkali land in the world. Nearly 1 / 5 of the cultivated land has been salinized. With the rapid development of the national economy and society, the contradiction between population growth and the decrease of cultivated land has become increasingly prominent. Various types of saline-alkali land have become important land reserve resources. It is an effective way to improve ecological and economic benefits by cultivating new salt-tolerant plant varieties, improving land utilization and reducing the impact of saline-alkali disasters. In recent years, a number of poplar hybrids that are fast-growing, drought-resistant, salt-alkali-resistant, and pest-resistant have been introduced at home and abroad. [0003] Wutun poplar is a nat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 邹吉祥金华李新林李琳琳王蕾吴萌王路路
Owner DALIAN NATIONALITIES UNIVERSITY
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