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Extraction method for blueberry anthocyanin

A blueberry anthocyanin and extraction method technology, which is applied in the field of blueberry anthocyanin extraction, can solve the problems of low extraction rate, high extraction operation difficulty and high extraction cost, and achieves the effects of simple and convenient operation, low cost and high purity

Active Publication Date: 2016-09-21
淄博普蓝农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the extraction methods of anthocyanins include solvent method, carbon dioxide supercritical extraction method, ultrasonic method, biological compound enzyme method, etc. These methods generally have the disadvantages of low extraction rate, difficult extraction operation, and high extraction cost.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The extraction method of described blueberry anthocyanin, the steps are as follows:

[0028] (1) Crushing: crush 500g of blueberry fresh fruit into blueberry pulp;

[0029] (2) Preparation of biological compound enzyme solution: first, add 1 g of citric acid to 100 g of water, then adjust the pH to 4.5 with sodium hydroxide, add 100 IU of acid cellulase, 100 IU of acid sucrase and 100 IU of tannase, and mix well , to obtain biological compound enzyme solution;

[0030] (3) Biological compound enzyme enzymatic hydrolysis: add the biological compound enzyme solution into the blueberry pulp for enzymolysis, the enzymatic hydrolysis temperature is 35°C, and the enzymatic hydrolysis time is 11 hours;

[0031] (4) Ultrasonic-assisted extraction: the enzymatically hydrolyzed substance obtained in step (3) was ultrasonically ultrasonicated for 15 minutes at 200W;

[0032] (5) Centrifuge to collect the supernatant: centrifuge the material obtained in step (4), remove the dregs...

Embodiment 2

[0036] The extraction method of described blueberry anthocyanin, the steps are as follows:

[0037] (1) Crushing: crush 500g of blueberry fresh fruit into blueberry pulp;

[0038] (2) Preparation of biological compound enzyme solution: first, add 1 g of citric acid to 100 g of water, then adjust the pH to 4.0 with sodium hydroxide, add 100 IU of acid cellulase, 100 IU of acid sucrase and 100 IU of tannase, and mix well , to obtain biological compound enzyme solution;

[0039] (3) Biological compound enzyme enzymatic hydrolysis: add the biological compound enzyme solution into the blueberry pulp for enzymolysis, the enzymatic hydrolysis temperature is 30°C, and the enzymatic hydrolysis time is 10 hours;

[0040] (4) Ultrasonic-assisted extraction: the enzymolyzed substance obtained in step (3) was ultrasonically ultrasonicated for 10 minutes at 200W;

[0041] (5) centrifuge to collect the supernatant: centrifuge the material obtained in step (4), remove the dregs therein, and...

Embodiment 3

[0045] The extraction method of described blueberry anthocyanin, the steps are as follows:

[0046] (1) Crushing: crush 500g of blueberry fresh fruit into blueberry pulp;

[0047] (2) Preparation of biological compound enzyme solution: first, add 1 g of citric acid to 100 g of water, then adjust the pH to 4.2 with sodium hydroxide, add 100 IU of acid cellulase, 100 IU of acid sucrase and 100 IU of tannase, and mix well , to obtain biological compound enzyme solution;

[0048] (3) Biological compound enzyme enzymatic hydrolysis: add the biological compound enzyme solution into the blueberry pulp for enzymolysis, the enzymatic hydrolysis temperature is 40°C, and the enzymatic hydrolysis time is 12 hours;

[0049] (4) Ultrasonic-assisted extraction: the enzymatically hydrolyzed substance obtained in step (3) was ultrasonically ultrasonicated for 20 minutes at 200W;

[0050] (5) centrifuge to collect the supernatant: centrifuge the material obtained in step (4), remove the dregs...

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PUM

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Abstract

The invention belongs to the technical field of extraction of natural products and in particular relates to an extraction method for blueberry anthocyanin. The extraction method comprises the following steps: firstly, crushing blueberries into blueberry pulp; then, adding a biological complex enzyme for enzymolysis; carrying out ultrasound-assisted extraction, centrifuging and collecting supernatant, carrying out ultrafiltration concentration and spray drying to obtain the anthocyanin. The biological complex enzyme is a compound of acidic cellulase, acidic sucrase and tannase. The blueberry anthocyanin extracted by adopting biological complex enzyme enzymolysis and ultrasound-assisted extraction manners is high in purity and low in cost, and the operation is simple and convenient; an extraction process is green and environmentally friendly; an anthocyanin product has no residues of toxic chemical reagents and the obtained anthocyanin is safer and more reliable; the extraction efficiency of the anthocyanin reaches 90 percent or more.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction, and in particular relates to a method for extracting blueberry anthocyanins. Background technique [0002] Anthocyanins are a class of water-soluble pigments that widely exist in plants and belong to flavonoid compounds. Anthocyanins, like other natural pigments, are non-toxic and have no side effects, have high safety performance, natural coloring and tone, and have health care functions. Anthocyanins are pure natural anti-aging nutritional supplements, and their antioxidant properties are 50 times higher than vitamin E and 200 times higher than vitamin C. [0003] Blueberry anthocyanins are the most effective antioxidant substances found in nature, which can be detected in the blood 20 minutes after taking it. Blueberry anthocyanins can not only prevent the early generation of wrinkles, but also maintain normal cell connections, stabilize blood vessels, enhance blood circul...

Claims

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Application Information

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IPC IPC(8): C07D311/62C09B61/00
CPCC07D311/62C09B61/00
Inventor 吴林徐振彪屈新宇
Owner 淄博普蓝农业科技有限公司
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