High-quality phage preservation protective agent and preparation method and application thereof

A phage, high-quality technology, applied in the field of biotechnology and microbial products, can solve the problems of restricting the development of phage application, short storage time of phage, and easy contamination of phage, and achieve convenient application, good adaptability, high survival rate and activity Effect

Inactive Publication Date: 2016-09-21
SUZHOU IRIVET BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The protective agents in the prior art often have poor preservation effect, the storage time of phage is short, the survival rate is low, phage is easily contaminated, and must be preserved under low temperature conditions, which seriously restricts the development of phage application

Method used

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  • High-quality phage preservation protective agent and preparation method and application thereof
  • High-quality phage preservation protective agent and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] A high-quality phage preservation and protection agent, by weight component, includes the following components:

[0048] Skimmed milk powder 5 parts

[0049] Albumin powder 3 parts

[0050] Yeast powder 0.5 part

[0051] Neutral white clay 0.02 parts

[0052] Charcoal powder 0.01 parts

[0053] Propolis 0.02 parts

[0054] 1 part lactose

[0055] Maltose 0.8 parts

[0056] Sodium sulfate 0.2 parts

[0057] Calcium chloride 0.5 parts

[0058] Magnesium chloride 0.3 parts

[0059] Dipotassium hydrogen phosphate 0.02 part

[0060] Sodium dihydrogen phosphate 0.04 parts

[0061] 20% glycerin 1 part

[0062] Span-80 0.01 parts

[0063] 100 parts of deionized water;

[0064] In addition, the above-mentioned high-quality phage preservation and protection agent also includes 0.1 parts of vitamins by weight components, and the vitamins include the following components by weight components:

[0065] Vitamin C 1 part

[0066] Vitamin E 3 parts

[0067] Vitamin A 0....

Embodiment 2

[0077] A high-quality phage preservation and protection agent, by weight component, includes the following components:

[0078] Skimmed milk powder 12 parts

[0079] Albumin powder 5 parts

[0080] Yeast powder 1.2 parts

[0081] Neutral white clay 0.1 parts

[0082] Charcoal powder 0.03 parts

[0083] Propolis 0.05 parts

[0084] Lactose 1.8 parts

[0085] Maltose 2 parts

[0086] Sodium sulfate 0.3 parts

[0087] Calcium chloride 1.5 parts

[0088] Magnesium chloride 0.8 parts

[0089] Dipotassium hydrogen phosphate 0.3 parts

[0090] Sodium dihydrogen phosphate 0.15 parts

[0091] 40% glycerin 3 parts

[0092] Span-80 0.08 parts

[0093] 100 parts of purified water;

[0094] In addition, the above-mentioned high-quality phage preservation and protection agent also includes 0.5 parts of vitamins by weight components, and the vitamins include the following components by weight components:

[0095] Vitamin C 3 parts

[0096] Vitamin E 8 servings

[0097] Vitami...

Embodiment 3

[0107] A high-quality phage preservation and protection agent, by weight component, includes the following components:

[0108] Skimmed milk powder 8 parts

[0109] Albumin powder 4 parts

[0110] Yeast powder 1 part

[0111] Neutral white clay 0.06 parts

[0112] Charcoal powder 0.02 parts

[0113] Propolis 0.03 parts

[0114] Lactose 1.5 parts

[0115] 1 part maltose

[0116] Sodium sulfate 0.25 parts

[0117] Calcium chloride 0.9 parts

[0118] Magnesium chloride 0.6 parts

[0119] Dipotassium hydrogen phosphate 0.2 part

[0120] Sodium dihydrogen phosphate 0.1 parts

[0121] 30% glycerin 2 parts

[0122] Span-80 0.016 parts

[0123] 100 parts of purified water;

[0124] In addition, the above-mentioned high-quality phage preservation and protection agent also includes 0.3 parts of vitamins by weight components, and the vitamins include the following components by weight components:

[0125] Vitamin C 2 parts

[0126] Vitamin E 6 servings

[0127] Vitamin A ...

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PUM

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Abstract

The invention provides a high-quality phage preservation protective agent and preparation and application methods thereof. According to the high-quality phage preservation protective agent, the components are reasonable, skimmed milk powder, albumin powder, yeast powder, neutral clay and the like form a framework of the whole preservation protective agent, the preservation protective agent contains various saccharides and salts, the components are reasonable, the raw materials are wide in source, easy to obtain and low in cost, the preservation protective agent can be used for preservation of various phages, and the adaptability is good. According to the preparation method of high-quality phage preservation protective agent, the method is reasonable, the process is simple and easy to operate, the efficiency is high, the application cost is low, and operation is simple and easy to achieve. The high-quality phage preservation protective agent has an extremely high application prospect, use is convenient, and application is convenient and rapid.

Description

technical field [0001] The invention belongs to the field of biotechnology and microbial products, and in particular relates to a high-quality phage preservation and protection agent, a preparation method and an application method of the high-quality phage preservation and protection agent. Background technique [0002] Phage is a general term for a class of viruses that can infect microorganisms such as bacteria, actinomycetes, and spirochetes. No toxicity to animals and plants. With the development of molecular biology technology, phage has been widely used in many fields such as disease diagnosis and water treatment. Phages have also become important molecular and genetic research tools due to their ability to insert genes into host DNA. Using phages, many ingenious experiments can be designed. [0003] However, in the above-mentioned processes, the phages need to be preserved, and during the preservation process of the phages, it is often necessary to add a preservati...

Claims

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Application Information

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IPC IPC(8): C12N7/00
CPCC12N7/00C12N2795/00011
Inventor 许维素宋增福陈彪
Owner SUZHOU IRIVET BIOTECH
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