Novel process for co-producing wheat polysaccharide and bioactive peptide through alcohol fermentation waste liquid
A technology for alcohol fermentation and fermentation waste liquid, which is applied in the directions of fermentation, microorganism-based methods, biochemical equipment and methods, etc., can solve problems such as co-production of wheat polysaccharides that have not yet been found, and achieve good environmental protection significance, economic value, and good application prospects. , the production process is simple and easy to implement
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Embodiment 1
[0024] A new process for co-producing wheat polysaccharides and active peptides using alcoholic fermentation waste liquid, comprising the following steps:
[0025] 1) Pretreatment of fermentation waste liquid: After filtering the molasses alcohol waste liquid through a coarse grid, filter it with a 0.25 μm ultrafiltration membrane, add 0.1% chitosan to the filtrate, and then vigorously stir it. After standing for 20 hours, the fermentation waste After ultrasonic treatment of the liquid for 5 minutes, filter to obtain the pretreated molasses alcohol waste liquid;
[0026] 2) One-stage fermentation of fermentation waste liquid: add 0.5% potato flour, 1% yeast extract, and 0.005% magnesium sulfate to the pretreated molasses alcohol waste liquid, adjust the pH after mixing to 6.2, and sterilize at 121°C After 20 minutes, the mixed solution was cooled to room temperature, and 5% Bacillus circulans seed solution was added to the mixed solution. The fermentation temperature was contr...
Embodiment 2
[0032] A new process for co-producing wheat polysaccharides and active peptides using alcoholic fermentation waste liquid, comprising the following steps:
[0033] 1) Pretreatment of fermentation waste liquid: After filtering the molasses alcohol waste liquid through a coarse grid, use a 0.25 μm ultrafiltration membrane to filter, add 0.1% chitosan to the filtrate, and then vigorously stir it, and after standing for 22 hours, the fermentation waste After ultrasonic treatment of the liquid for 5 minutes, filter to obtain the pretreated molasses alcohol waste liquid;
[0034] 2) One-stage fermentation of fermentation waste liquid: add 1.5% potato flour, 1.5% yeast extract, and 0.005% magnesium sulfate to the pretreated molasses alcohol waste liquid, adjust the pH after mixing to 6.8, and sterilize at 121°C After 20 minutes, the mixed solution was cooled to room temperature, and 5%-8% Bacillus circulans seed solution was added to the mixed solution. The fermentation temperature w...
Embodiment 3
[0040] A new process for co-producing wheat polysaccharides and active peptides using alcoholic fermentation waste liquid, comprising the following steps:
[0041] 1) Pretreatment of fermentation waste liquid: After filtering the molasses alcohol waste liquid through a coarse grid, filter it with a 0.25 μm ultrafiltration membrane, add 0.1% chitosan to the filtrate, and then vigorously stir it. After standing for 24 hours, the fermentation waste After ultrasonic treatment of the liquid for 5 minutes, filter to obtain the pretreated molasses alcohol waste liquid;
[0042] 2) One-stage fermentation of fermentation waste liquid: add 0.5%-2% potato flour, 2% yeast extract, and 0.005% magnesium sulfate to the pretreated molasses alcohol waste liquid, adjust the pH after mixing to 7.5, 121°C Sterilize under high temperature for 20 minutes, cool the mixed solution to room temperature, add 8% Bacillus circulans seed solution to the mixed solution, control the fermentation temperature ...
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