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A kind of sio2-dna nanometer material and its preparation method and application

A technology of nanomaterials and nanospheres, applied in the field of nanofluorescent probes, can solve the problems of aggregation fluorescence quenching, affecting the detection process, reducing the application range of ordinary fluorescent probes, etc., and achieving good biocompatibility

Inactive Publication Date: 2018-03-02
QUFU NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Fluorescence detection technology has many advantages such as high sensitivity, low noise and wide dynamic range. However, the previous fluorescent probes often have some shortcomings. Fluorescence intensity changes nonlinearly, or aggregation fluorescence quenching occurs during aggregation, which affects the detection process. The existence of these shortcomings greatly reduces the application range of ordinary fluorescent probes. The discovery of aggregation-induced emission (AIE) phenomenon is a solution This question provides a way

Method used

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  • A kind of sio2-dna nanometer material and its preparation method and application
  • A kind of sio2-dna nanometer material and its preparation method and application
  • A kind of sio2-dna nanometer material and its preparation method and application

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Embodiment 1

[0030] Add 4mL TEOS to the mixed system of 3.3mL ammonia water and 47mL absolute ethanol, stir at room temperature for 24 hours, then add 300mL APTES, stir vigorously for at least 10 hours, centrifuge and wash with ethanol and deionized water several times to obtain the aminated 80nm SiO 2 nanospheres, figure 2 is its transmission electron microscope image.

Embodiment 2

[0032] The first step of AIE molecular synthesis: synthesis of tetraphenylethylene derivatives (TPE) 1, in an inclined three-necked flask, add p-methoxybenzophenone (1.06g, 5.0mmol), 1.34 times the equivalent of TiCl 3 / AlCl 3 (5.81g, 6.7mmol), 25 times the equivalent of zinc powder (8.01g, 122.0mmol), 100ml THF (easy to dry and remove water), heat and reflux for 20h. The reaction mixture was cooled to room temperature and filtered. The filtrate was evaporated to dryness, and the crude product was separated and purified by column chromatography (eluent: n-hexane / dichloromethane at a ratio of 3:1), with a yield of 91%.

[0033] The second step of AIE molecular synthesis: synthesis of tetraphenylethylene derivative (TPE) 2, add 1.40 g (3.56 mmol) product 1 and 20 ml methylene chloride to a 100 ml inclined three-necked flask, then place the flask in an acetone-liquid nitrogen In the container, the temperature inside the container can reach -78°C. A mixture of 3.59 g (14.3 mmol...

Embodiment 3

[0035]Example 3: First, add 9uL ss-DNA to 20uL 0.4mg / mL aminated silica sphere solution and disperse in the solution system at a concentration of 130nM. After incubating at 37°C for 30 minutes, add buffer solution and centrifuge at 12000rpm After 20 minutes, the dispersed ss-DNA was removed from the obtained supernatant, and then buffer solution was added to re-disperse for further use. Then add a series of concentrations of PSA (0-180ng / mL) and incubate at 25°C for 30 minutes, then add AIE molecules (final concentration of 5.5ug / mL), and finally add buffer solution to make the total volume reach 100uL, at 25°C Incubate for 15 minutes and put into a fluorometer for detection.

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Abstract

The invention belongs to the field of nano fluorescent probes and in particular relates to a SiO2-DNA nano material as well as a preparation method and application thereof. A DNA single chain in the SiO2-DNA nano material covers the surface of an ammoniated SiO2 nano-sphere; the grain diameter of the ammoniated SiO2 nano-sphere is 60nm to 200nm and the sequence of the DNA is 5'-ATGCTATAATATTAAT-3'. The SiO2-DNA nano material has the advantages of good biocompatibility, green and pollution-free properties, high luminous intensity, uneasiness of having a fluorescence quenching phenomenon and the like, so that the SiO2-DNA nano material can be applied to a biological system and can be used for determining a fluorescent signal.

Description

technical field [0001] The invention belongs to the field of nano fluorescent probes, in particular to a SiO 2 –DNA nanomaterials and their preparation methods and applications. Background technique [0002] Fluorescence detection technology has many advantages such as high sensitivity, low noise and wide dynamic range. However, the previous fluorescent probes often have some shortcomings. Fluorescence intensity changes nonlinearly, or aggregation fluorescence quenching occurs during aggregation, which affects the detection process. The existence of these shortcomings greatly reduces the application range of ordinary fluorescent probes. The discovery of aggregation-induced emission (AIE) phenomenon is a solution This question offers one way. Firstly, molecules with AIE performance will not undergo fluorescence quenching in the aggregation state; secondly, the fluorescence spectra of most AIE molecules will not shift with the change of their concentration; thirdly, AIE fluo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/06B82Y30/00B82Y20/00G01N21/64
CPCB82Y20/00B82Y30/00C09K11/06G01N21/6402
Inventor 渠凤丽张小宾赵岩裴海盟杜蕙同
Owner QUFU NORMAL UNIV
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