Chinese angelica acidic polysaccharide component, and preparation method and application thereof

A technology of acidic polysaccharides and extraction methods, applied in the direction of organic active ingredients, medical preparations containing active ingredients, drug combinations, etc., can solve problems such as activity differences, and achieve the goal of improving tolerance, significant anti-radiation effect, and enhancing immunity The effect of action

Inactive Publication Date: 2016-10-05
FOURTH MILITARY MEDICAL UNIVERSITY
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Problems solved by technology

[0011] According to the above, it can be seen that there are many polysaccharide components of Angelica sinensis isolated at pres

Method used

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  • Chinese angelica acidic polysaccharide component, and preparation method and application thereof
  • Chinese angelica acidic polysaccharide component, and preparation method and application thereof
  • Chinese angelica acidic polysaccharide component, and preparation method and application thereof

Examples

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[0050] Example 1

[0051] Weigh 1kg of angelica root and pulverize it into coarse powder; reflux and extract with 4000mL of absolute ethanol for 2 hours (degreased), filter out the ethanol extract, and treat the angelica root residue (hereinafter also referred to as medicinal residue) according to the above method again , The ethanol extract was filtered out, the residue was evaporated to dry the ethanol, and after drying at 40°C, it was refluxed with 6000mL 1.0mol / L NaOH for 3 times, 2h each time. The water extracts were combined and concentrated to 1000 mL under reduced pressure on a 60°C rotary evaporator. After precooling at 4°C for 4 hours, 2 times volume of absolute ethanol was added. After standing at 4°C for 12 hours, the precipitate was centrifuged at 4000g for 15 minutes. The precipitate was dissolved in 400 mL of water, freeze-thaw repeated 5 times, centrifuged, and the precipitate was discarded; the Sevag method (ie V Polysaccharide solution : V Chloroform : V N-but...

Example Embodiment

[0052] Example 2

[0053] Weigh 1kg of Angelica root and pulverize it into coarse powder; use 8000mL of 95% ethanol for reflux extraction for 2 hours, filter out the ethanol extract, evaporate the ethanol from the medicine residue, and dry at 50℃, then use 8000mL of 1.2mol / L NaOH for reflux extraction twice , 3h each time. The water extracts were combined and concentrated to 800 mL under reduced pressure on a rotary evaporator at 60°C. After precooling at 4°C for 6 hours, 2 times volume of absolute ethanol was added. After standing at 4°C for 12 hours, centrifuged at 3000g for 15 minutes to collect the precipitate. The precipitate was dissolved in 300 mL of water, freeze-thaw repeatedly 10 times, centrifuged, and the precipitate was discarded. The aqueous solution is dialyzed against distilled water for 48 hours with a dialysis membrane with a molecular weight cut-off of 8000Da to remove small molecular impurities. The solution in the dialysis bag is collected and concentrated t...

Example Embodiment

[0054] Example 3

[0055] Weigh 1kg of Angelica root and pulverize it into coarse powder; wet with 2000mL 95% ethanol; percolate with 8000ml 95% ethanol, evaporate the dregs of ethanol, dry at 50℃, and extract twice with 8000mL 1.0mol / L KOH reflux , 3h each time. The water extracts were combined and concentrated to 800 mL under reduced pressure on a rotary evaporator at 60°C. After precooling at 4°C for 6 hours, 3 times the volume of absolute ethanol was added. After standing at 4°C for 12 hours, the precipitate was centrifuged at 3000g for 10 minutes. The precipitate was dissolved in 300 mL of water, freeze-thaw was repeated 5 times, centrifuged, and the precipitate was discarded. Add calcium hydroxide alkaline solution to adjust pH 11, place overnight at 4°C, and filter with suction. Adjust the filtrate to pH 5 with sulfuric acid solution, place overnight at 4°C, and then filter with suction. Add alkaline solution of KOH to the filtrate to adjust pH 7. The aqueous solution use...

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Abstract

The invention relates to a Chinese angelica acidic polysaccharide component, a preparation method thereof, and an application thereof in the preparation of drugs and health products for preventing cancers, enhancing immunity and preventing radiation damages, and belongs to the field of traditional Chinese medicine modernization. The method comprises the following steps: degreasing Chinese angelica roots, carrying out refluxing extraction with alkaline water, precipitating polysaccharides with alcohol, removing proteins, carrying out dialysis, carrying out anion exchange column DEAE serial column grading, eluting a part size exclusion chromatograph with 0.5mol/L of NaCl, further purifying, carrying out reduced pressure concentration on the above obtained 50kD or above component, and freeze-drying the obtained concentrate to obtain the Chinese angelica acidic polysaccharide component. The Chinese angelica acidic polysaccharide component has substantial cancer prevention, immunity enhancement and radiation damage prevention effects.

Description

technical field [0001] The invention relates to an angelica acidic polysaccharide component, an extraction method, and its application in the preparation of anti-cancer, immune-enhancing, and anti-radiation damage drugs, belonging to the field of traditional Chinese medicine modernization. Background technique [0002] Angelica sinensis is the dried root of Angelica sinensis (Oliv.) Diels, a famous and commonly used traditional Chinese medicine. Scholars at home and abroad have analyzed and studied the chemical components of Angelica Sinensis using modern extraction, separation and content determination methods, and found that the main active components of Angelica Sinensis are polysaccharides and volatile oils. In the past two decades, due to the development of molecular and cell biology, people have gradually realized that polysaccharides and their complexes have extremely important biological functions. Studies have found that Angelica polysaccharides have antithrombotic...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P35/00A61P35/02A61P37/04A61P39/00
Inventor 曹蔚李小强李卫燕王四旺周暄宣张雅
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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