Heat-resisting cryoprotectant for classical swine fever live vaccines and preparation method and application thereof

A live swine fever vaccine, heat-resistant freeze-drying technology, applied in the field of veterinary biological products manufacturing, can solve problems such as side reactions, achieve the effects of increasing stability, efficiently protecting antigen activity, and reducing the risk of inactivation

Active Publication Date: 2016-10-12
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention aims at the problem that the existing heat-resistant freeze-drying protective agent used for swine fever live vaccines has side effects caused by exogenous protein and gelatin, and provides a non-exogenous protein and gelatin-resistant lyoprotectan

Method used

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  • Heat-resisting cryoprotectant for classical swine fever live vaccines and preparation method and application thereof
  • Heat-resisting cryoprotectant for classical swine fever live vaccines and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1 heat-resistant lyoprotectant 1

[0022] The heat-resistant lyoprotectant 1 is composed of the following components in mass percentage: 0.5% inulin (polymerization degree (DP) is 9), 5% dextran (Dextran-70), polyvinylpyrrolidone (PVP K30 ) 3%, lactose 5%, sucrose 2%, polyethylene glycol (PEG 200) 1%, histidine 0.3%, cysteine ​​1.5%, DMEM medium (Gibco company) 3%, the rest is water for injection .

[0023] The specific preparation steps of the heat-resistant lyoprotectant 1 are as follows:

[0024] (1) Weigh inulin (DP is 9), dextran (Dextran-70), polyvinylpyrrolidone (PVP K30), lactose, sucrose, polyethylene glycol (PEG200) and dissolve in a small amount of water for injection, and steam Sterilize for 30 minutes, control the temperature at 110°C during the sterilization process, and set aside;

[0025] (2) Weigh histidine, cysteine ​​and DMEM culture medium and dissolve in a small amount of water for injection, and use a filter membrane with a pore size ...

Embodiment 2

[0029] Embodiment 2 heat-resistant lyoprotectant 2

[0030] The heat-resistant lyoprotectant 2 is composed of the following components in mass percentage: inulin (DP 5) 2%, dextran (Dextran-70) 10%, polyvinylpyrrolidone (PVP K90) 2%, Lactose 1%, sucrose 0.5%, polyethylene glycol (PEG400) 2%, histidine 0.01%, cysteine ​​3%, DMEM medium 0.5%, and the rest is water for injection.

[0031] The specific preparation steps of the heat-resistant lyoprotectant 2 are as follows:

[0032] (1) Weigh inulin (DP 5), dextran (Dextran-70), polyvinylpyrrolidone (PVP K90), lactose, sucrose, polyethylene glycol (PEG400) and dissolve them in a small amount of water for injection. Sterilize for 30 minutes, control the temperature at 110°C during the sterilization process, and set aside;

[0033] (2) Weigh histidine, cysteine ​​and DMEM and dissolve them in a small amount of water for injection, filter and sterilize them with a filter membrane with a pore size of 0.22 μm for later use;

[0034] ...

Embodiment 3

[0037] Embodiment 3 swine fever novel heat-resistant lyoprotectant 3

[0038]Novel heat-resistant lyoprotectant 3 for swine fever, consisting of the following components in mass percentage: inulin (DP 2) 0.2%, dextran (Dextran-40) 1%, polyvinylpyrrolidone (PVP K30) 5%, lactose 10%, sucrose 5%, polyethylene glycol (PEG 250) 0.1%, histidine 0.5%, cysteine ​​0.1%, DMEM medium 1.5%, and the rest is water for injection.

[0039] The specific preparation steps of the heat-resistant lyoprotectant 3 are as follows:

[0040] (1) Weigh inulin, dextran (Dextran-40), polyvinylpyrrolidone (PVP K30), lactose, sucrose, and polyethylene glycol, dissolve them in a small amount of water for injection, and sterilize them by autoclaving for 30 minutes. During the sterilization process, the temperature is controlled at 110°C for standby;

[0041] (2) Weigh histidine, cysteine ​​and DMEM and dissolve them in a small amount of water for injection, filter and sterilize them with a filter membrane w...

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Abstract

The invention provides heat-resisting cryoprotectant for classical swine fever live vaccines and a preparation method and application thereof, and belongs to the technical field of veterinary biological product manufacturing. The cryoprotectant is prepared from, by mass, 0.2-2.5% of synanthrin, 0.5-12% of glucan, 1-6% of polyvinylpyrrolidone, 0.5-12% of lactose, 0.2-6% of saccharose, 0.05-2.5% of polyethylene glycol, 0.005-0.6% of histidine, 0.05-4% of cysteine and 0.2-4% of a DMEM culture medium, and the rest is water for injection. The invention further provides a preparation method and application of the heat-resisting cryoprotectant. The heat-resisting cryoprotectant does not contain foreign protein or gelatin, stress reactions and side effects on animals are avoided, the safety of the vaccines is greatly improved, the antigen activity can be efficiently protected in the freeze-drying and storing process, and the stability of the vaccines is improved.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological product manufacturing, and in particular relates to a heat-resistant freeze-drying protectant for swine fever live vaccine, a preparation method and application thereof. Background technique [0002] Classical swine fever (CSF), commonly known as "rotten intestinal fever", is also known as hog cholera (hog cholera), swine fever or classical swine fever in some parts of Europe and the United States. Classical Swine Fever Virus (CSFV) is an acute, febrile and highly contagious infectious disease, clinically characterized by acute onset, persistent high fever, generalized spotting and splenic infarction. The high mortality rate of swine fever will cause huge safety hazards and economic losses to the pig industry. [0003] In 1908, Hutyra, Hungary prepared CSFV hyperimmune serum, which can produce strong protection. However, due to the high price of hyperimmune serum, it is difficult to...

Claims

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Application Information

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IPC IPC(8): A61K47/36A61K47/32A61K47/26A61K47/34A61K47/22A61K47/20A61K39/187A61P31/14
CPCA61K39/12A61K47/10A61K47/183A61K47/22A61K47/26A61K47/32A61K47/36A61K2039/525A61K2039/552C12N2770/24034
Inventor 吕芳赵艳红卢宇彭苗苗邓碧华张金秋候继波
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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