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Method for separating phenylalanine, tyrosine and tryptophan racemates

A technology of tryptophan racemate and phenylalanine, applied in the field of separation of D, to achieve high separation effect, simple operation, cheap and easy to obtain the effect of sample and reagent dosage

Inactive Publication Date: 2016-10-12
SHENYANG INSTITUTE OF CHEMICAL TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report at home and abroad on the separation of D, L-amino acid racemates by capillary electrophoresis with dual chiral selectors

Method used

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  • Method for separating phenylalanine, tyrosine and tryptophan racemates
  • Method for separating phenylalanine, tyrosine and tryptophan racemates
  • Method for separating phenylalanine, tyrosine and tryptophan racemates

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Embodiment

[0024] 1. Accurately weigh 10 mg each of phenylalanine, tyrosine, and tryptophan racemate, put them in 10 mL brown volumetric flasks, dissolve with methanol, adjust the volume to the mark, and shake well. A stock solution with a concentration of 1 mg / mL was obtained for each sample, and -20 oC Store in the refrigerator. Precisely measure 1 mL of each sample stock solution into 10 mL brown volumetric flasks, dilute to the mark with methanol, and shake well to obtain 100 μg / mL test solution, which is filtered through a 0.45 μm microporous membrane for use.

[0025] 2. Prepare 5 mmol / L NaH 2 PO 4 Containing 8 mmol / L β-cyclodextrin, 10 mmol / L copper acetate and 13 mmol / L-histidine as running buffer, with H 3 PO 4 and NaOH to adjust the pH to 5.0, filtered through a 0.45 μm microporous membrane, and ultrasonically degassed for later use. The capillary column was washed successively with 0.1 mol / L NaOH, twice distilled water and the above-mentioned running buffer for 10 minute...

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Abstract

The invention relates to a method for separating phenylalanine, tyrosine and tryptophan racemates, in particular to a method for separating D,L-amino acid racemates. Cu and L-histidine are used as chiral selectors, beta-cyclodextrin is used as a separating medium, disodium hydrogen phosphate is used as a buffering solution, a ligand exchange method is used so that a ternary complex can be formed through left-handed and right-handed rotation of Cu, L-histidine and a pantozol drug, under the action of the separating medium beta-cyclodextrin, enantiomers are separated through a capillary electrophoresis apparatus, the D,L-amino acid racemates are separated through a ligand exchange capillary electrophoresis method, and Cu, L-histidine and beta-cyclodextrin are used as the chiral selectors, wherein beta-cyclodextrin is used as the separating medium. The method is easy to operate, the chiral selectors are low in price and easy to obtain, dosage of a sample and reagents is small, and the method can be used for in vivo analysis of amino acids and detection of product optical purity.

Description

technical field [0001] The invention relates to a method for separating D, L-amino acid racemates, in particular to a method for separating phenylalanine, tyrosine and tryptophan racemates. Background technique [0002] Capillary electrophoresis is also called high-efficiency capillary electrophoresis because of its advantages of high efficiency, rapidity and simplicity. It is a new electrophoresis technology that is driven by a high-voltage DC electric field and separates charged particles in a capillary according to their distribution coefficients. It has multiple separation modes, high separation column efficiency, fast analysis speed, and easy operation. , less consumption of samples and reagents, less environmental pollution, etc., so it is favored in chiral separation research. [0003] There are many kinds of chiral selectors in this method. Common selectors include cyclodextrin, chiral crown ether, macrocyclic glycopeptide antibiotics, linear polysaccharides, protei...

Claims

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Application Information

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IPC IPC(8): B01D57/02
CPCB01D57/02
Inventor 关瑾邵会丽阎峰石爽王思林
Owner SHENYANG INSTITUTE OF CHEMICAL TECHNOLOGY
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